Fermentation of raffinose by lactose-fermenting strains of Yersinia enterocolitica and by sucrose-fermenting strains of Escherichia coli

G Cornelis, R K Luke, M H Richmond

    Research output: Contribution to journalArticle

    Abstract

    Introduction of plasmids carrying the lacY gene (lactose permease gene) into Yersinia enterocolitica results in cells being able to ferment both lactose and raffinose. Transfer of such plasmids into Escherichia coli C600 (lacY) confers ability to ferment lactose but not raffinose. Derivatives of C600 that ferment both lactose and sucrose (Lac+ Scr+ strains) are able to ferment raffinose, but do not grow well on raffinose minimal medium. Fermentation of raffinose by Lac+ strains of Y. enterocolitica, and by Lac+ Scr+ strains of E. coli, is explained in terms of transport of raffinose via the lac permease and subsequent breakdown catalyzed by invertase.
    Original languageEnglish
    Pages (from-to)180-3
    Number of pages4
    JournalJournal of clinical microbiology
    Volume7
    Issue number2
    Publication statusPublished - 1978

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