Effect of leukocyte- and platelet-rich fibrin (L-PRF) on bone regeneration

A study in rabbits

Michel Knapen, Damien Gheldof, Pierre Drion, Pierre Layrolle, Eric Rompen, France Lambert

Research output: Contribution to journalArticle

Abstract

Background: The positive effect of leukocyte- and platelet-rich fibrin (L-PRF) on osteogenesis has been widely described in vitro. However, clinical and preclinical studies are very little and controversial in demonstrating a significant beneficial effect of L-PRF in bone regeneration. Purpose: The goal of the present study was to compare the potential effect of L-PRF in a standardized model. Materials and Methods: A total of 72 hemispheres were implanted on the calvaria of 18 rabbits and filled with three different space fillers: L-PRF, bovine hydroxyapatite (BHA), BHA+L-PRF, and an empty hemisphere was used as control. Six rabbits were sacrificed at three distinct time points: 1 week, 5 weeks, and 12 weeks. Histological and histomorphometrical analyses were carried out. Results: At the early phase of bone regeneration (1 week), from a descriptive analysis, a higher proportion of connective tissue colonized the regeneration chamber in the two groups containing BHA particles. Nevertheless, no statistical differences were found within the four groups in terms of bone quantity and quality at each timepoint (p=.3623). Conclusions: According to the present study, L-PRF does not seem to provide any additional effect on the kinetics, quality, and quantity of bone in the present model of guided bone regeneration.

Original languageEnglish
JournalClinical Implant Dentistry and Related Research
DOIs
Publication statusAccepted/In press - 9 Sep 2013

Fingerprint

Bone Regeneration
Fibrin
Leukocytes
Blood Platelets
Rabbits
Durapatite
Bone and Bones
Osteogenesis
Skull
Connective Tissue
Regeneration

Keywords

  • Bone regeneration
  • Histomorphometry
  • L-PRF
  • Osteoconduction

Cite this

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title = "Effect of leukocyte- and platelet-rich fibrin (L-PRF) on bone regeneration: A study in rabbits",
abstract = "Background: The positive effect of leukocyte- and platelet-rich fibrin (L-PRF) on osteogenesis has been widely described in vitro. However, clinical and preclinical studies are very little and controversial in demonstrating a significant beneficial effect of L-PRF in bone regeneration. Purpose: The goal of the present study was to compare the potential effect of L-PRF in a standardized model. Materials and Methods: A total of 72 hemispheres were implanted on the calvaria of 18 rabbits and filled with three different space fillers: L-PRF, bovine hydroxyapatite (BHA), BHA+L-PRF, and an empty hemisphere was used as control. Six rabbits were sacrificed at three distinct time points: 1 week, 5 weeks, and 12 weeks. Histological and histomorphometrical analyses were carried out. Results: At the early phase of bone regeneration (1 week), from a descriptive analysis, a higher proportion of connective tissue colonized the regeneration chamber in the two groups containing BHA particles. Nevertheless, no statistical differences were found within the four groups in terms of bone quantity and quality at each timepoint (p=.3623). Conclusions: According to the present study, L-PRF does not seem to provide any additional effect on the kinetics, quality, and quantity of bone in the present model of guided bone regeneration.",
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Effect of leukocyte- and platelet-rich fibrin (L-PRF) on bone regeneration : A study in rabbits. / Knapen, Michel; Gheldof, Damien; Drion, Pierre; Layrolle, Pierre; Rompen, Eric; Lambert, France.

In: Clinical Implant Dentistry and Related Research, 09.09.2013.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of leukocyte- and platelet-rich fibrin (L-PRF) on bone regeneration

T2 - A study in rabbits

AU - Knapen, Michel

AU - Gheldof, Damien

AU - Drion, Pierre

AU - Layrolle, Pierre

AU - Rompen, Eric

AU - Lambert, France

PY - 2013/9/9

Y1 - 2013/9/9

N2 - Background: The positive effect of leukocyte- and platelet-rich fibrin (L-PRF) on osteogenesis has been widely described in vitro. However, clinical and preclinical studies are very little and controversial in demonstrating a significant beneficial effect of L-PRF in bone regeneration. Purpose: The goal of the present study was to compare the potential effect of L-PRF in a standardized model. Materials and Methods: A total of 72 hemispheres were implanted on the calvaria of 18 rabbits and filled with three different space fillers: L-PRF, bovine hydroxyapatite (BHA), BHA+L-PRF, and an empty hemisphere was used as control. Six rabbits were sacrificed at three distinct time points: 1 week, 5 weeks, and 12 weeks. Histological and histomorphometrical analyses were carried out. Results: At the early phase of bone regeneration (1 week), from a descriptive analysis, a higher proportion of connective tissue colonized the regeneration chamber in the two groups containing BHA particles. Nevertheless, no statistical differences were found within the four groups in terms of bone quantity and quality at each timepoint (p=.3623). Conclusions: According to the present study, L-PRF does not seem to provide any additional effect on the kinetics, quality, and quantity of bone in the present model of guided bone regeneration.

AB - Background: The positive effect of leukocyte- and platelet-rich fibrin (L-PRF) on osteogenesis has been widely described in vitro. However, clinical and preclinical studies are very little and controversial in demonstrating a significant beneficial effect of L-PRF in bone regeneration. Purpose: The goal of the present study was to compare the potential effect of L-PRF in a standardized model. Materials and Methods: A total of 72 hemispheres were implanted on the calvaria of 18 rabbits and filled with three different space fillers: L-PRF, bovine hydroxyapatite (BHA), BHA+L-PRF, and an empty hemisphere was used as control. Six rabbits were sacrificed at three distinct time points: 1 week, 5 weeks, and 12 weeks. Histological and histomorphometrical analyses were carried out. Results: At the early phase of bone regeneration (1 week), from a descriptive analysis, a higher proportion of connective tissue colonized the regeneration chamber in the two groups containing BHA particles. Nevertheless, no statistical differences were found within the four groups in terms of bone quantity and quality at each timepoint (p=.3623). Conclusions: According to the present study, L-PRF does not seem to provide any additional effect on the kinetics, quality, and quantity of bone in the present model of guided bone regeneration.

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