Construction of 12 EST libraries and characterization of a 12,226 EST dataset for chicory (Cichorium intybus) root, leaves and nodules in the context of carbohydrate metabolism investigation

Nicolas Dauchot, Dominique Mingeot, Bénédicte Purnelle, Céline Muys, Bernard Watillon, Marc Boutry, Pierre Van Cutsem

Research output: Contribution to journalArticle

Abstract

The industrial chicory, Cichorium intybus, is a member of the Asteraceae family that accumulates fructan of the inulin type in its root. Inulin is a low calories sweetener, a texture agent and a health promoting ingredient due to its prebiotic properties. Average inulin chain length is a critical parameter that is genotype and temperature dependent. In the context of the study of carbohydrate metabolism and to get insight into the transcriptome of chicory root and to visualize temporal changes of gene expression during the growing season, we obtained and characterized 10 cDNA libraries from chicory roots regularly sampled in field during a growing season. A leaf and a nodule libraries were also obtained for comparison.
Original languageEnglish
Number of pages14
JournalBMC plant biology
Volume9
DOIs
Publication statusPublished - 2009

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Keywords

  • Polymorphism, Single Nucleotide
  • Root Nodules, Plant
  • Sequence Analysis, DNA
  • Genome, Plant
  • Gene Expression Profiling
  • Plant Leaves
  • Seasons
  • Chicory
  • Databases, Genetic
  • RNA, Plant
  • Cluster Analysis
  • Expressed Sequence Tags
  • Carbohydrate Metabolism
  • Gene Library

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