Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe

Valerie Migeot, Damien Hermand

Research output: Contribution to journalArticle

Abstract

The distribution of modified histones within the fission yeast Schizosaccharomyces pombe genome is ultimately dependent upon the transcriptional activity and in turn influences the ability of the polymerases to bind and progress through the chromatin template. The Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) method currently provides the highest resolution, accuracy, and reproducibility to characterize histones modifications within a defined region of the genome. The following protocol details the method applied to S. pombe.

Original languageEnglish
Pages (from-to)25-34
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume1721
DOIs
Publication statusPublished - 2018

Keywords

  • Journal Article
  • Modifications
  • Polymerase
  • Chromatin
  • Fission yeast
  • Histone

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