Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe

Research output: Contribution to journalArticle

Abstract

The distribution of modified histones within the fission yeast Schizosaccharomyces pombe genome is ultimately dependent upon the transcriptional activity and in turn influences the ability of the polymerases to bind and progress through the chromatin template. The Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) method currently provides the highest resolution, accuracy, and reproducibility to characterize histones modifications within a defined region of the genome. The following protocol details the method applied to S. pombe.

Original languageEnglish
Pages (from-to)25-34
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume1721
DOIs
Publication statusPublished - 2018
Externally publishedYes

Fingerprint

Schizosaccharomyces
Chromatin Immunoprecipitation
Ubiquitination
Acetylation
Methylation
Polymerase Chain Reaction
Histone Code
Genome
Histones
Chromatin

Keywords

  • Journal Article
  • Modifications
  • Polymerase
  • Chromatin
  • Fission yeast
  • Histone

Cite this

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title = "Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe",
abstract = "The distribution of modified histones within the fission yeast Schizosaccharomyces pombe genome is ultimately dependent upon the transcriptional activity and in turn influences the ability of the polymerases to bind and progress through the chromatin template. The Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) method currently provides the highest resolution, accuracy, and reproducibility to characterize histones modifications within a defined region of the genome. The following protocol details the method applied to S. pombe.",
keywords = "Journal Article, Modifications, Polymerase, Chromatin, Fission yeast, Histone",
author = "Valerie Migeot and Damien Hermand",
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journal = "Methods in molecular biology (Clifton, N.J.)",
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AU - Hermand, Damien

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AB - The distribution of modified histones within the fission yeast Schizosaccharomyces pombe genome is ultimately dependent upon the transcriptional activity and in turn influences the ability of the polymerases to bind and progress through the chromatin template. The Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) method currently provides the highest resolution, accuracy, and reproducibility to characterize histones modifications within a defined region of the genome. The following protocol details the method applied to S. pombe.

KW - Journal Article

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KW - Polymerase

KW - Chromatin

KW - Fission yeast

KW - Histone

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