Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe

Valerie Migeot; Damien Hermand

doi:10.1007/978-1-4939-7546-4_3

Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe

Valerie Migeot, Damien Hermand

URPHYM

Research output: Contribution to journal › Article › peer-review

Abstract

The distribution of modified histones within the fission yeast Schizosaccharomyces pombe genome is ultimately dependent upon the transcriptional activity and in turn influences the ability of the polymerases to bind and progress through the chromatin template. The Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) method currently provides the highest resolution, accuracy, and reproducibility to characterize histones modifications within a defined region of the genome. The following protocol details the method applied to S. pombe.

Original language	English
Pages (from-to)	25-34
Number of pages	10
Journal	Methods in Molecular Biology
Volume	1721
DOIs	https://doi.org/10.1007/978-1-4939-7546-4_3
Publication status	Published - 2018

Keywords

Journal Article
Modifications
Polymerase
Chromatin
Fission yeast
Histone

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10.1007/978-1-4939-7546-4_3

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title = "Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe",

abstract = "The distribution of modified histones within the fission yeast Schizosaccharomyces pombe genome is ultimately dependent upon the transcriptional activity and in turn influences the ability of the polymerases to bind and progress through the chromatin template. The Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) method currently provides the highest resolution, accuracy, and reproducibility to characterize histones modifications within a defined region of the genome. The following protocol details the method applied to S. pombe.",

keywords = "Journal Article, Modifications, Polymerase, Chromatin, Fission yeast, Histone",

author = "Valerie Migeot and Damien Hermand",

year = "2018",

doi = "10.1007/978-1-4939-7546-4_3",

language = "English",

volume = "1721",

pages = "25--34",

journal = "Methods in Molecular Biology",

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AU - Migeot, Valerie

AU - Hermand, Damien

PY - 2018

Y1 - 2018

N2 - The distribution of modified histones within the fission yeast Schizosaccharomyces pombe genome is ultimately dependent upon the transcriptional activity and in turn influences the ability of the polymerases to bind and progress through the chromatin template. The Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) method currently provides the highest resolution, accuracy, and reproducibility to characterize histones modifications within a defined region of the genome. The following protocol details the method applied to S. pombe.

AB - The distribution of modified histones within the fission yeast Schizosaccharomyces pombe genome is ultimately dependent upon the transcriptional activity and in turn influences the ability of the polymerases to bind and progress through the chromatin template. The Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) method currently provides the highest resolution, accuracy, and reproducibility to characterize histones modifications within a defined region of the genome. The following protocol details the method applied to S. pombe.

KW - Journal Article

KW - Modifications

KW - Polymerase

KW - Chromatin

KW - Fission yeast

KW - Histone

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U2 - 10.1007/978-1-4939-7546-4_3

DO - 10.1007/978-1-4939-7546-4_3

M3 - Article

C2 - 29423844

SN - 1064-3745

VL - 1721

SP - 25

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe

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Keywords

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