Cell death by autoschizis in TRAMP prostate carcinoma cells as a result of treatment by ascorbate: Menadione combination

Jacques Gilloteaux; James M. Jamison; Deborah R. Neal

doi:10.1080/01913120590951239

Cell death by autoschizis in TRAMP prostate carcinoma cells as a result of treatment by ascorbate: Menadione combination

Jacques Gilloteaux, James M. Jamison, Deborah R. Neal

URPHYM

Research output: Contribution to journal › Article › peer-review

Abstract

A prostate carcinoma cell line derived from the transgenic murine prostate cancer model (TRAMP) was treated with ascorbate (VC) alone, menadione (VK ₃) alone, or a combination of ascorbate:menadione (VC + VK ₃) for 1, 2, and 4h. Cytotoxic cell alterations examined by light and electron microscopy were treatment-dependent with VC + VK₃ > VC > VK₃. Induced by oxidative stress, these alterations included cytokeletal changes conducive to cytoplasmic blebbing, self-excisions, and progressive nuclear alterations. While the excised parts contained ribosomes, they were devoid of nuclear fragments or other organelles. The organelle-free self-excisions caused an extreme reduction in cell size as well as chromatolysis and karyolysis that were consistent with cell death by autoschizis, but not with apoptosis.

Original language	English
Pages (from-to)	221-235
Number of pages	15
Journal	Ultrastructural Pathology
Volume	29
Issue number	3-4
DOIs	https://doi.org/10.1080/01913120590951239
Publication status	Published - May 2005

Keywords

Ascorbate
Autoschizis
Cell death
Microscopy
Nuclease
Prostate carcinoma cells
Ribonucleoproteins
TRAMP

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1080/01913120590951239

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title = "Cell death by autoschizis in TRAMP prostate carcinoma cells as a result of treatment by ascorbate: Menadione combination",

abstract = "A prostate carcinoma cell line derived from the transgenic murine prostate cancer model (TRAMP) was treated with ascorbate (VC) alone, menadione (VK 3) alone, or a combination of ascorbate:menadione (VC + VK 3) for 1, 2, and 4h. Cytotoxic cell alterations examined by light and electron microscopy were treatment-dependent with VC + VK3 > VC > VK3. Induced by oxidative stress, these alterations included cytokeletal changes conducive to cytoplasmic blebbing, self-excisions, and progressive nuclear alterations. While the excised parts contained ribosomes, they were devoid of nuclear fragments or other organelles. The organelle-free self-excisions caused an extreme reduction in cell size as well as chromatolysis and karyolysis that were consistent with cell death by autoschizis, but not with apoptosis.",

keywords = "Ascorbate, Autoschizis, Cell death, Microscopy, Nuclease, Prostate carcinoma cells, Ribonucleoproteins, TRAMP",

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year = "2005",

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doi = "10.1080/01913120590951239",

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T1 - Cell death by autoschizis in TRAMP prostate carcinoma cells as a result of treatment by ascorbate

T2 - Menadione combination

AU - Gilloteaux, Jacques

AU - Jamison, James M.

AU - Neal, Deborah R.

PY - 2005/5

Y1 - 2005/5

N2 - A prostate carcinoma cell line derived from the transgenic murine prostate cancer model (TRAMP) was treated with ascorbate (VC) alone, menadione (VK 3) alone, or a combination of ascorbate:menadione (VC + VK 3) for 1, 2, and 4h. Cytotoxic cell alterations examined by light and electron microscopy were treatment-dependent with VC + VK3 > VC > VK3. Induced by oxidative stress, these alterations included cytokeletal changes conducive to cytoplasmic blebbing, self-excisions, and progressive nuclear alterations. While the excised parts contained ribosomes, they were devoid of nuclear fragments or other organelles. The organelle-free self-excisions caused an extreme reduction in cell size as well as chromatolysis and karyolysis that were consistent with cell death by autoschizis, but not with apoptosis.

AB - A prostate carcinoma cell line derived from the transgenic murine prostate cancer model (TRAMP) was treated with ascorbate (VC) alone, menadione (VK 3) alone, or a combination of ascorbate:menadione (VC + VK 3) for 1, 2, and 4h. Cytotoxic cell alterations examined by light and electron microscopy were treatment-dependent with VC + VK3 > VC > VK3. Induced by oxidative stress, these alterations included cytokeletal changes conducive to cytoplasmic blebbing, self-excisions, and progressive nuclear alterations. While the excised parts contained ribosomes, they were devoid of nuclear fragments or other organelles. The organelle-free self-excisions caused an extreme reduction in cell size as well as chromatolysis and karyolysis that were consistent with cell death by autoschizis, but not with apoptosis.

KW - Ascorbate

KW - Autoschizis

KW - Cell death

KW - Microscopy

KW - Nuclease

KW - Prostate carcinoma cells

KW - Ribonucleoproteins

KW - TRAMP

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ER -

Cell death by autoschizis in TRAMP prostate carcinoma cells as a result of treatment by ascorbate: Menadione combination

Abstract

Keywords

UN SDGs

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