Assignment of acetyl groups to O-2 and/or O-3 of pectic oligogalacturonides using negative electrospray ionization ion trap mass spectrometry

Bernard Quéméner; Juan Carlos Cabrera Pino; Marie-Christine Ralet; Estelle Bonnin; Jean-François Thibault

doi:10.1002/jms.478

Assignment of acetyl groups to O-2 and/or O-3 of pectic oligogalacturonides using negative electrospray ionization ion trap mass spectrometry

Bernard Quéméner, Juan Carlos Cabrera Pino, Marie-Christine Ralet, Estelle Bonnin, Jean-François Thibault

Unit of Research in Plant Cellular and Molecular Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Partially acetylated and methylated oligogalacturonides produced by enzymatic hydrolysis of sugar beet pectin were analysed by negative electrospray ionization ion trap mass spectrometry (ESI-ITMS). The (18)O labelling of the oligomer reducing end allowed the precise assignment of the fragments resulting from glycosidic bond and cross-ring cleavages. The collisional-induced dissociation of the C(i) and Z(j) fragment ions through sequential MS(n) experiments always displayed (0, 2)A-type cross-ring cleavage ions which were related to C(2)H(4)O(2) losses. These (0, 2)A ions appeared to be highly diagnostic ions allowing the precise location of the acetyl groups to the O-2 and/or O-3 of the acetylated galacturonic acid residues.

Original language	English
Pages (from-to)	641-8
Number of pages	8
Journal	Journal of mass spectrometry : JMS
Volume	38
Issue number	6
DOIs	https://doi.org/10.1002/jms.478
Publication status	Published - Jun 2003

Keywords

Spectrometry, Mass, Electrospray Ionization
Acetylation
Pectins
Oligosaccharides
Beta vulgaris
Molecular Sequence Data
Carbohydrate Sequence

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10.1002/jms.478

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title = "Assignment of acetyl groups to O-2 and/or O-3 of pectic oligogalacturonides using negative electrospray ionization ion trap mass spectrometry",

abstract = "Partially acetylated and methylated oligogalacturonides produced by enzymatic hydrolysis of sugar beet pectin were analysed by negative electrospray ionization ion trap mass spectrometry (ESI-ITMS). The (18)O labelling of the oligomer reducing end allowed the precise assignment of the fragments resulting from glycosidic bond and cross-ring cleavages. The collisional-induced dissociation of the C(i) and Z(j) fragment ions through sequential MS(n) experiments always displayed (0, 2)A-type cross-ring cleavage ions which were related to C(2)H(4)O(2) losses. These (0, 2)A ions appeared to be highly diagnostic ions allowing the precise location of the acetyl groups to the O-2 and/or O-3 of the acetylated galacturonic acid residues.",

keywords = "Spectrometry, Mass, Electrospray Ionization, Acetylation, Pectins, Oligosaccharides, Beta vulgaris, Molecular Sequence Data, Carbohydrate Sequence",

author = "Bernard Qu{\'e}m{\'e}ner and {Cabrera Pino}, {Juan Carlos} and Marie-Christine Ralet and Estelle Bonnin and Jean-Fran{\c c}ois Thibault",

year = "2003",

month = jun,

doi = "10.1002/jms.478",

language = "English",

volume = "38",

pages = "641--8",

journal = "Journal of mass spectrometry : JMS",

issn = "1076-5174",

publisher = "John Wiley and Sons Ltd",

number = "6",

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TY - JOUR

T1 - Assignment of acetyl groups to O-2 and/or O-3 of pectic oligogalacturonides using negative electrospray ionization ion trap mass spectrometry

AU - Quéméner, Bernard

AU - Cabrera Pino, Juan Carlos

AU - Ralet, Marie-Christine

AU - Bonnin, Estelle

AU - Thibault, Jean-François

PY - 2003/6

Y1 - 2003/6

N2 - Partially acetylated and methylated oligogalacturonides produced by enzymatic hydrolysis of sugar beet pectin were analysed by negative electrospray ionization ion trap mass spectrometry (ESI-ITMS). The (18)O labelling of the oligomer reducing end allowed the precise assignment of the fragments resulting from glycosidic bond and cross-ring cleavages. The collisional-induced dissociation of the C(i) and Z(j) fragment ions through sequential MS(n) experiments always displayed (0, 2)A-type cross-ring cleavage ions which were related to C(2)H(4)O(2) losses. These (0, 2)A ions appeared to be highly diagnostic ions allowing the precise location of the acetyl groups to the O-2 and/or O-3 of the acetylated galacturonic acid residues.

AB - Partially acetylated and methylated oligogalacturonides produced by enzymatic hydrolysis of sugar beet pectin were analysed by negative electrospray ionization ion trap mass spectrometry (ESI-ITMS). The (18)O labelling of the oligomer reducing end allowed the precise assignment of the fragments resulting from glycosidic bond and cross-ring cleavages. The collisional-induced dissociation of the C(i) and Z(j) fragment ions through sequential MS(n) experiments always displayed (0, 2)A-type cross-ring cleavage ions which were related to C(2)H(4)O(2) losses. These (0, 2)A ions appeared to be highly diagnostic ions allowing the precise location of the acetyl groups to the O-2 and/or O-3 of the acetylated galacturonic acid residues.

KW - Spectrometry, Mass, Electrospray Ionization

KW - Acetylation

KW - Pectins

KW - Oligosaccharides

KW - Beta vulgaris

KW - Molecular Sequence Data

KW - Carbohydrate Sequence

U2 - 10.1002/jms.478

DO - 10.1002/jms.478

M3 - Article

C2 - 12827633

SN - 1076-5174

VL - 38

SP - 641

EP - 648

JO - Journal of mass spectrometry : JMS

JF - Journal of mass spectrometry : JMS

IS - 6

ER -

Assignment of acetyl groups to O-2 and/or O-3 of pectic oligogalacturonides using negative electrospray ionization ion trap mass spectrometry

Abstract

Keywords

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