Assignment of acetyl groups to O-2 and/or O-3 of pectic oligogalacturonides using negative electrospray ionization ion trap mass spectrometry

Bernard Quéméner, Juan Carlos Cabrera Pino, Marie-Christine Ralet, Estelle Bonnin, Jean-François Thibault

Research output: Contribution to journalArticlepeer-review

Abstract

Partially acetylated and methylated oligogalacturonides produced by enzymatic hydrolysis of sugar beet pectin were analysed by negative electrospray ionization ion trap mass spectrometry (ESI-ITMS). The (18)O labelling of the oligomer reducing end allowed the precise assignment of the fragments resulting from glycosidic bond and cross-ring cleavages. The collisional-induced dissociation of the C(i) and Z(j) fragment ions through sequential MS(n) experiments always displayed (0, 2)A-type cross-ring cleavage ions which were related to C(2)H(4)O(2) losses. These (0, 2)A ions appeared to be highly diagnostic ions allowing the precise location of the acetyl groups to the O-2 and/or O-3 of the acetylated galacturonic acid residues.
Original languageEnglish
Pages (from-to)641-8
Number of pages8
JournalJournal of mass spectrometry : JMS
Volume38
Issue number6
DOIs
Publication statusPublished - Jun 2003

Keywords

  • Spectrometry, Mass, Electrospray Ionization
  • Acetylation
  • Pectins
  • Oligosaccharides
  • Beta vulgaris
  • Molecular Sequence Data
  • Carbohydrate Sequence

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