Assignment of acetyl groups to O-2 and/or O-3 of pectic oligogalacturonides using negative electrospray ionization ion trap mass spectrometry

Bernard Quéméner, Juan Carlos Cabrera Pino, Marie-Christine Ralet, Estelle Bonnin, Jean-François Thibault

Research output: Contribution to journalArticle

Abstract

Partially acetylated and methylated oligogalacturonides produced by enzymatic hydrolysis of sugar beet pectin were analysed by negative electrospray ionization ion trap mass spectrometry (ESI-ITMS). The (18)O labelling of the oligomer reducing end allowed the precise assignment of the fragments resulting from glycosidic bond and cross-ring cleavages. The collisional-induced dissociation of the C(i) and Z(j) fragment ions through sequential MS(n) experiments always displayed (0, 2)A-type cross-ring cleavage ions which were related to C(2)H(4)O(2) losses. These (0, 2)A ions appeared to be highly diagnostic ions allowing the precise location of the acetyl groups to the O-2 and/or O-3 of the acetylated galacturonic acid residues.
Original languageEnglish
Pages (from-to)641-8
Number of pages8
JournalJournal of mass spectrometry : JMS
Volume38
Issue number6
DOIs
Publication statusPublished - Jun 2003

Fingerprint

Electrospray ionization
Mass spectrometry
Ions
Sugar beets
Enzymatic hydrolysis
Oligomers
Labeling
Experiments

Keywords

  • Spectrometry, Mass, Electrospray Ionization
  • Acetylation
  • Pectins
  • Oligosaccharides
  • Beta vulgaris
  • Molecular Sequence Data
  • Carbohydrate Sequence

Cite this

@article{cf1d009bb89a4782a47a22fad19588bc,
title = "Assignment of acetyl groups to O-2 and/or O-3 of pectic oligogalacturonides using negative electrospray ionization ion trap mass spectrometry",
abstract = "Partially acetylated and methylated oligogalacturonides produced by enzymatic hydrolysis of sugar beet pectin were analysed by negative electrospray ionization ion trap mass spectrometry (ESI-ITMS). The (18)O labelling of the oligomer reducing end allowed the precise assignment of the fragments resulting from glycosidic bond and cross-ring cleavages. The collisional-induced dissociation of the C(i) and Z(j) fragment ions through sequential MS(n) experiments always displayed (0, 2)A-type cross-ring cleavage ions which were related to C(2)H(4)O(2) losses. These (0, 2)A ions appeared to be highly diagnostic ions allowing the precise location of the acetyl groups to the O-2 and/or O-3 of the acetylated galacturonic acid residues.",
keywords = "Spectrometry, Mass, Electrospray Ionization, Acetylation, Pectins, Oligosaccharides, Beta vulgaris, Molecular Sequence Data, Carbohydrate Sequence",
author = "Bernard Qu{\'e}m{\'e}ner and {Cabrera Pino}, {Juan Carlos} and Marie-Christine Ralet and Estelle Bonnin and Jean-Fran{\cc}ois Thibault",
note = "Copyright 2003 John Wiley & Sons, Ltd.",
year = "2003",
month = "6",
doi = "10.1002/jms.478",
language = "English",
volume = "38",
pages = "641--8",
journal = "Journal of mass spectrometry : JMS",
issn = "1076-5174",
publisher = "John Wiley and Sons Ltd",
number = "6",

}

Assignment of acetyl groups to O-2 and/or O-3 of pectic oligogalacturonides using negative electrospray ionization ion trap mass spectrometry. / Quéméner, Bernard; Cabrera Pino, Juan Carlos; Ralet, Marie-Christine; Bonnin, Estelle; Thibault, Jean-François.

In: Journal of mass spectrometry : JMS, Vol. 38, No. 6, 06.2003, p. 641-8.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Assignment of acetyl groups to O-2 and/or O-3 of pectic oligogalacturonides using negative electrospray ionization ion trap mass spectrometry

AU - Quéméner, Bernard

AU - Cabrera Pino, Juan Carlos

AU - Ralet, Marie-Christine

AU - Bonnin, Estelle

AU - Thibault, Jean-François

N1 - Copyright 2003 John Wiley & Sons, Ltd.

PY - 2003/6

Y1 - 2003/6

N2 - Partially acetylated and methylated oligogalacturonides produced by enzymatic hydrolysis of sugar beet pectin were analysed by negative electrospray ionization ion trap mass spectrometry (ESI-ITMS). The (18)O labelling of the oligomer reducing end allowed the precise assignment of the fragments resulting from glycosidic bond and cross-ring cleavages. The collisional-induced dissociation of the C(i) and Z(j) fragment ions through sequential MS(n) experiments always displayed (0, 2)A-type cross-ring cleavage ions which were related to C(2)H(4)O(2) losses. These (0, 2)A ions appeared to be highly diagnostic ions allowing the precise location of the acetyl groups to the O-2 and/or O-3 of the acetylated galacturonic acid residues.

AB - Partially acetylated and methylated oligogalacturonides produced by enzymatic hydrolysis of sugar beet pectin were analysed by negative electrospray ionization ion trap mass spectrometry (ESI-ITMS). The (18)O labelling of the oligomer reducing end allowed the precise assignment of the fragments resulting from glycosidic bond and cross-ring cleavages. The collisional-induced dissociation of the C(i) and Z(j) fragment ions through sequential MS(n) experiments always displayed (0, 2)A-type cross-ring cleavage ions which were related to C(2)H(4)O(2) losses. These (0, 2)A ions appeared to be highly diagnostic ions allowing the precise location of the acetyl groups to the O-2 and/or O-3 of the acetylated galacturonic acid residues.

KW - Spectrometry, Mass, Electrospray Ionization

KW - Acetylation

KW - Pectins

KW - Oligosaccharides

KW - Beta vulgaris

KW - Molecular Sequence Data

KW - Carbohydrate Sequence

U2 - 10.1002/jms.478

DO - 10.1002/jms.478

M3 - Article

C2 - 12827633

VL - 38

SP - 641

EP - 648

JO - Journal of mass spectrometry : JMS

JF - Journal of mass spectrometry : JMS

SN - 1076-5174

IS - 6

ER -