Virulence Factors in Klebsiella pneumoniae: Detection by Multiplex PCR and Correlation with Clinical Patterns

Résumé

Klebsiella pneumoniae is a Gram-negative enterobacter ubiquity in the environment and commensal of the intestinal and respiratory tract in human. This bacteria induces more often nosocomial infections as pneumonia, urinary or abdominal infections and septicemia. In the 1980’s, a new variant of Klebsiella pneumoniae called hypervirulent has emerged in South-East of Asia causing a syndrome of pyogenic liver abscess. Its principal characteristic is the capacity to give metastasis in young and healthy patients. This hypervirulence comes from some particular genes said virulence factors. Already widely studied, few clear associations between factors and clinical data have been established nowadays. Multiplex PCRs allow to detect some virulence genes have been developed from these described in the publications of Turton et al. (2010) and Tang et al. (2010). Strains collections of Klebsiella pneumoniae from different origins (Belgium, Republic Democratic of Congo, Cambodia and Burkina Faso) have been analyzed with these updated PCRs targeting 12 virulence factors. The clinical data relatives to each patient were collected and linked with the virulence profile of the corresponding strain. A depth analysis of these data allowed confirming potential association between type of infections and the presence of some virulence factors already cited in the literature but had not established new relationship.

la date de réponse	2016
langue originale	Anglais
L'institution diplômante	Universite de Namur
Superviseur	Pierre Bogaerts (Promoteur) & Xavier De Bolle (Copromoteur)