Tagging of the BmaC adhesin in Bruce/la abortus

  • Alexandre Bonnet

Student thesis: Master typesMaster en sciences biomédicales

Résumé

Bacteria from the Brucella genus are intracellular pathogens responsible for brucellosis a worldwide anthropozoonotic disease. It was previously shown that, to infect HeLa cells, Brucella must adhere to the surface of the host cell. So far, three adhesins of Brucella have been identified (BmaC, BtaE, BtaF) and shown to bind to extracellular matrix components. During this work, we have decided to concentrate on BmaC, a monomeric autotransporter that binds to fibronectin .
lt has recently been shown that the infectiosity of Brucella varies along its bacterial cell cycle where the newly generated daughter bacteria (the "newborns") mostly infect host cells. Our hypothesis is that the newborns could preferentially express adhesins as compared to others bacterial cell types. To test this , we tagged BmaC with a mye epitope by allelic replacement in Brucella abortus and we tried to detect the presence of the protein on the bacterial surface by performing immunofluorescence against the myc-tag . Until now, the myc-BmaC fusion is undetectable, probably because of its very low production level
la date de réponse2014
langue originaleAnglais
L'institution diplômante
  • Universite de Namur
SuperviseurXavier De Bolle (Promoteur)

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