Study of the rote of ABCB5 in melanoma-initiating cells: Generation oftwo mouse models that express ABCB5-IRESeGFP and spontaneously develop melanoma

Résumé

Melanoma is one of three most common skin cancers, the others being basal cell cancer and squamous cell cancer. Cutaneous melanoma stemmedfrom neoplastic melanocytes, which are known to express the ABCB5 transporter ABCB5 is, a member of the subfamily B of ABC transporter that was identified as a marker of melanoma initiating cells. However, these data remain highly controversial. In order to clarify that, we generated a mouse mode/ spontaneously developing melanoma and expressing ABCB5 coupled to the GFP. The long-term goal is to check if the paradigm of the Cancer Stem Cells is applicable in the case of melanoma and to validate whether ABCB5 is a marker of melanoma initiating cells. Mapping ABCB5 prote in expression in the animal is important to improve the current knowledge on the localization of the transporter. More important/y, this information will allow us to speculate on ils potential role in normal organisms as well as in pathological conditions. We have worked on the conditions ofGFP detection in ABCB5-IRESeGFP mice as well as of several staining including Fontana-Masson and Cresyl Violet to targe! melanin and neurons in substantia nigra, respective/y. Although we could not detect GFP in mouse brain sections, our data suggest that ABCB5 is expressed at the level of enterocytes.

la date de réponse	2014
langue originale	Anglais
L'institution diplômante	Universite de Namur