Validation of the calibrated thrombin generation test (cTGT) as the reference assay to evaluate the procoagulant activity of nanomaterials

J. Laloy; S. Robert; C. Marbehant; F. Mullier; Jorge Humberto Mejia Mendoza; J.-P. Piret; S. Lucas; B. Chatelain; J.-M. Dogné; O. Toussaint; B. Masereel; S. Rolin

doi:10.3109/17435390.2011.569096

Validation of the calibrated thrombin generation test (cTGT) as the reference assay to evaluate the procoagulant activity of nanomaterials

J. Laloy, S. Robert, C. Marbehant, F. Mullier, Jorge Humberto Mejia Mendoza, J.-P. Piret, S. Lucas, B. Chatelain, J.-M. Dogné, O. Toussaint, B. Masereel, S. Rolin

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Résumé

We validated a preclinical toxicological screening assay and provided guidelines to evaluate the potential impact of nanoparticles (NPs) on blood coagulation. Five NPs with various physicochemical properties were studied using several existing methods of clotting times and thrombin generation assays in human normal pool plasma. In both recalcification clotting time (RCT) and calibrated thrombin generation test (cTGT), the NPs exhibited procoagulant activity (SiO2 ≥ SiC ≥ TiC > CuO > CB) but cTGT was more sensitive and relevant than RCT. Thus, the cTGT appears as a reference assay to investigate the nanoparticle (NP) procoagulant activity in human plasma. It should be used as the reference toxicity test for evaluating the effects of nanomaterials on coagulation cascade. In addition, we also showed that the use of the Pluronic F-108 dispersant and/or the sonication for the NP suspension preparation may mask their procoagulant activity and thus should be avoided.

langue originale	Anglais
Pages (de - à)	213-232
Nombre de pages	20
journal	Nanotoxicology
Volume	6
Numéro de publication	2
Les DOIs	https://doi.org/10.3109/17435390.2011.569096
Etat de la publication	Publié - 1 mars 2012

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Laloy, J., Robert, S., Marbehant, C., Mullier, F., Mejia Mendoza, J. H., Piret, J.-P., Lucas, S., Chatelain, B., Dogné, J.-M., Toussaint, O., Masereel, B., & Rolin, S. (2012). Validation of the calibrated thrombin generation test (cTGT) as the reference assay to evaluate the procoagulant activity of nanomaterials. Nanotoxicology, 6(2), 213-232. https://doi.org/10.3109/17435390.2011.569096

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title = "Validation of the calibrated thrombin generation test (cTGT) as the reference assay to evaluate the procoagulant activity of nanomaterials",

abstract = "We validated a preclinical toxicological screening assay and provided guidelines to evaluate the potential impact of nanoparticles (NPs) on blood coagulation. Five NPs with various physicochemical properties were studied using several existing methods of clotting times and thrombin generation assays in human normal pool plasma. In both recalcification clotting time (RCT) and calibrated thrombin generation test (cTGT), the NPs exhibited procoagulant activity (SiO2 ≥ SiC ≥ TiC > CuO > CB) but cTGT was more sensitive and relevant than RCT. Thus, the cTGT appears as a reference assay to investigate the nanoparticle (NP) procoagulant activity in human plasma. It should be used as the reference toxicity test for evaluating the effects of nanomaterials on coagulation cascade. In addition, we also showed that the use of the Pluronic F-108 dispersant and/or the sonication for the NP suspension preparation may mask their procoagulant activity and thus should be avoided.",

author = "J. Laloy and S. Robert and C. Marbehant and F. Mullier and {Mejia Mendoza}, {Jorge Humberto} and J.-P. Piret and S. Lucas and B. Chatelain and J.-M. Dogn{\'e} and O. Toussaint and B. Masereel and S. Rolin",

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Laloy, J, Robert, S, Marbehant, C, Mullier, F , Mejia Mendoza, JH, Piret, J-P, Lucas, S , Chatelain, B , Dogné, J-M , Toussaint, O , Masereel, B & Rolin, S 2012, 'Validation of the calibrated thrombin generation test (cTGT) as the reference assay to evaluate the procoagulant activity of nanomaterials', Nanotoxicology, VOL. 6, Numéro 2, p. 213-232. https://doi.org/10.3109/17435390.2011.569096

TY - JOUR

T1 - Validation of the calibrated thrombin generation test (cTGT) as the reference assay to evaluate the procoagulant activity of nanomaterials

AU - Laloy, J.

AU - Robert, S.

AU - Marbehant, C.

AU - Mullier, F.

AU - Mejia Mendoza, Jorge Humberto

AU - Piret, J.-P.

AU - Lucas, S.

AU - Chatelain, B.

AU - Dogné, J.-M.

AU - Toussaint, O.

AU - Masereel, B.

AU - Rolin, S.

N1 - MEDLINE® is the source for the MeSH terms of this document.

PY - 2012/3/1

Y1 - 2012/3/1

N2 - We validated a preclinical toxicological screening assay and provided guidelines to evaluate the potential impact of nanoparticles (NPs) on blood coagulation. Five NPs with various physicochemical properties were studied using several existing methods of clotting times and thrombin generation assays in human normal pool plasma. In both recalcification clotting time (RCT) and calibrated thrombin generation test (cTGT), the NPs exhibited procoagulant activity (SiO2 ≥ SiC ≥ TiC > CuO > CB) but cTGT was more sensitive and relevant than RCT. Thus, the cTGT appears as a reference assay to investigate the nanoparticle (NP) procoagulant activity in human plasma. It should be used as the reference toxicity test for evaluating the effects of nanomaterials on coagulation cascade. In addition, we also showed that the use of the Pluronic F-108 dispersant and/or the sonication for the NP suspension preparation may mask their procoagulant activity and thus should be avoided.

AB - We validated a preclinical toxicological screening assay and provided guidelines to evaluate the potential impact of nanoparticles (NPs) on blood coagulation. Five NPs with various physicochemical properties were studied using several existing methods of clotting times and thrombin generation assays in human normal pool plasma. In both recalcification clotting time (RCT) and calibrated thrombin generation test (cTGT), the NPs exhibited procoagulant activity (SiO2 ≥ SiC ≥ TiC > CuO > CB) but cTGT was more sensitive and relevant than RCT. Thus, the cTGT appears as a reference assay to investigate the nanoparticle (NP) procoagulant activity in human plasma. It should be used as the reference toxicity test for evaluating the effects of nanomaterials on coagulation cascade. In addition, we also showed that the use of the Pluronic F-108 dispersant and/or the sonication for the NP suspension preparation may mask their procoagulant activity and thus should be avoided.

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Validation of the calibrated thrombin generation test (cTGT) as the reference assay to evaluate the procoagulant activity of nanomaterials

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