Validation of the boronate sensor ContPY1 as a specific probe for fluorescent detection of hydrogen peroxide in plants

Quentin Ledoux, Pascal Veys, Pierre Van Cutsem, Sergio Mauro, Fabio Lucaccioni, Istvan Marko

    Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs

    4 Téléchargements (Pure)

    Résumé

    Studying the implication of hydrogen peroxide in biological processes in plants remains a challenge due to the current shortcomings of H2O2-responsive probes. The use of ContPY1, a new fluorescent probe, which is highly selective and sensitive for H2O2, was investigated. To validate the use of ContPY1 on plants, we have generated protocols employing cells suspensions and leaves, and measured specifically H2O2 production by plants using spectrofluorometry and microscopy.

    langue originaleAnglais
    Numéro d'articlee26827
    journalPlant Signaling and Behavior
    Volume8
    Numéro de publication11
    Les DOIs
    Etat de la publicationPublié - 3 déc. 2013

    Empreinte digitale

    Examiner les sujets de recherche de « Validation of the boronate sensor ContPY1 as a specific probe for fluorescent detection of hydrogen peroxide in plants ». Ensemble, ils forment une empreinte digitale unique.

    Contient cette citation