Validation of the boronate sensor ContPY1 as a specific probe for fluorescent detection of hydrogen peroxide in plants

Quentin Ledoux; Pascal Veys; Pierre Van Cutsem; Sergio Mauro; Fabio Lucaccioni; Istvan Marko

doi:10.4161/psb.26827

Validation of the boronate sensor ContPY1 as a specific probe for fluorescent detection of hydrogen peroxide in plants

Quentin Ledoux, Pascal Veys, Pierre Van Cutsem, Sergio Mauro, Fabio Lucaccioni, Istvan Marko

Unite de recherche en biologie cellulaire vegetale

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

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Résumé

Studying the implication of hydrogen peroxide in biological processes in plants remains a challenge due to the current shortcomings of H₂O₂-responsive probes. The use of ContPY1, a new fluorescent probe, which is highly selective and sensitive for H₂O₂, was investigated. To validate the use of ContPY1 on plants, we have generated protocols employing cells suspensions and leaves, and measured specifically H₂O₂ production by plants using spectrofluorometry and microscopy.

langue originale	Anglais
Numéro d'article	e26827
journal	Plant Signaling and Behavior
Volume	8
Numéro de publication	11
Les DOIs	https://doi.org/10.4161/psb.26827
Etat de la publication	Publié - 3 déc. 2013

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10.4161/psb.26827

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title = "Validation of the boronate sensor ContPY1 as a specific probe for fluorescent detection of hydrogen peroxide in plants",

abstract = "Studying the implication of hydrogen peroxide in biological processes in plants remains a challenge due to the current shortcomings of H2O2-responsive probes. The use of ContPY1, a new fluorescent probe, which is highly selective and sensitive for H2O2, was investigated. To validate the use of ContPY1 on plants, we have generated protocols employing cells suspensions and leaves, and measured specifically H2O2 production by plants using spectrofluorometry and microscopy.",

keywords = "Arabidopsis, Cells suspension, ContPY1, Fluorescence, Hydrogen peroxide, Microscopy, Protoplasts",

author = "Quentin Ledoux and Pascal Veys and {Van Cutsem}, Pierre and Sergio Mauro and Fabio Lucaccioni and Istvan Marko",

year = "2013",

month = dec,

day = "3",

doi = "10.4161/psb.26827",

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journal = "Plant Signaling and Behavior",

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T1 - Validation of the boronate sensor ContPY1 as a specific probe for fluorescent detection of hydrogen peroxide in plants

AU - Ledoux, Quentin

AU - Veys, Pascal

AU - Van Cutsem, Pierre

AU - Mauro, Sergio

AU - Lucaccioni, Fabio

AU - Marko, Istvan

PY - 2013/12/3

Y1 - 2013/12/3

N2 - Studying the implication of hydrogen peroxide in biological processes in plants remains a challenge due to the current shortcomings of H2O2-responsive probes. The use of ContPY1, a new fluorescent probe, which is highly selective and sensitive for H2O2, was investigated. To validate the use of ContPY1 on plants, we have generated protocols employing cells suspensions and leaves, and measured specifically H2O2 production by plants using spectrofluorometry and microscopy.

AB - Studying the implication of hydrogen peroxide in biological processes in plants remains a challenge due to the current shortcomings of H2O2-responsive probes. The use of ContPY1, a new fluorescent probe, which is highly selective and sensitive for H2O2, was investigated. To validate the use of ContPY1 on plants, we have generated protocols employing cells suspensions and leaves, and measured specifically H2O2 production by plants using spectrofluorometry and microscopy.

KW - Arabidopsis

KW - Cells suspension

KW - ContPY1

KW - Fluorescence

KW - Hydrogen peroxide

KW - Microscopy

KW - Protoplasts

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U2 - 10.4161/psb.26827

DO - 10.4161/psb.26827

M3 - Article

AN - SCOPUS:84890925783

SN - 1559-2316

VL - 8

JO - Plant Signaling and Behavior

JF - Plant Signaling and Behavior

IS - 11

M1 - e26827

ER -

Validation of the boronate sensor ContPY1 as a specific probe for fluorescent detection of hydrogen peroxide in plants

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