Unseen riverine risk: Spatio-temporal shifts of microplastic pollution and its bioavailability in freshwater fish within the Ikopa River urban system

Growing concern over microplastic pollution, driven by their widespread accumulation in the environment, stresses the need for comprehensive assessments. This study investigates the spatial and temporal distribution of microplastics in the Ikopa River (Antananarivo – Madagascar), which flows through a densely populated area, and examines their correlation with contamination levels in local fish species. By analyzing upstream and downstream stations across wet and dry seasons, only a notable increase in microplastic concentration downstream during the wet season was observed, ranging from 138.6 ± 9.0 to 222.0 ± 24.5 particles m⁻³, with polyethylene-co-vinyl acetate being the predominant polymer at 62.3 ± 5.13% of the total sampled polymers. This distribution underlines the impact of urban activities on pollution levels. Fish species, gambusia and Nile tilapia, were assessed for microplastic occurrence in gills and gastrointestinal tracts. Higher contamination rates were found in gambusia, enlightening the influence of feeding behaviour and fish habitat on microplastics contamination. Ingestion of microplastics directly from the water column was evident in both species, with the detection of high-density plastics such as polytetrafluoroethylene and polyvinyl chloride suggesting likely sediment contamination. This research highlights the widespread contamination of aquatic environments and its direct impact on local wildlife, pointing to a clear requirement for effective pollution management strategies.