Résumé

Thiolated peptides bearing the Ile-Gly-Asp (IGD) motif, a highly conserved sequence of fibronectin, are used for the preparation of anisotropic self-assembled monolayers (SAM gradients) to study the whole-population migratory behavior of metastatic breast cancer cells (MDA-MB-231 cells). Ile-Gly-Asp-Gln-(IGDQ)-exposing SAMs sustain the adhesion of MDA-MB-231 cells by triggering focal adhesion kinase phosphorylation, similarly to the analogous Gly-Arg-Gly-Asp-(GRGD)-terminating surfaces. However, the biological responses of different cell lines interfaced with the SAM gradients show that only those exposing the IGDQ sequence induce significant migration of MDA-MB-231 cells. In particular, the observed migratory behavior suggests the presence of cell subpopulations associated with a "stationary" or a "migratory" phenotype, the latter determining a considerable cell migration at the sub-cm length scale. These findings are of great importance as they suggest for the first time an active role of biological surfaces exposing the IGD motif in the multicomponent orchestration of cellular signaling involved in the metastatic progression.

langue originaleAnglais
Pages (de - à)321-329
Nombre de pages9
journalSmall
Volume12
Numéro de publication3
Les DOIs
étatPublié - 2016

Empreinte digitale

Peptides
Bearings (structural)
Adhesion
Cells
Cell signaling
Focal Adhesion Protein-Tyrosine Kinases
Phosphorylation
Self assembled monolayers
Fibronectins
Neoplasms
glycyl-arginyl-glycyl-aspartic acid
Conserved Sequence
Cell Movement
Breast Neoplasms
Phenotype
Cell Line
Population

Citer ceci

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title = "Unleashing Cancer Cells on Surfaces Exposing Motogenic IGDQ Peptides",
abstract = "Thiolated peptides bearing the Ile-Gly-Asp (IGD) motif, a highly conserved sequence of fibronectin, are used for the preparation of anisotropic self-assembled monolayers (SAM gradients) to study the whole-population migratory behavior of metastatic breast cancer cells (MDA-MB-231 cells). Ile-Gly-Asp-Gln-(IGDQ)-exposing SAMs sustain the adhesion of MDA-MB-231 cells by triggering focal adhesion kinase phosphorylation, similarly to the analogous Gly-Arg-Gly-Asp-(GRGD)-terminating surfaces. However, the biological responses of different cell lines interfaced with the SAM gradients show that only those exposing the IGDQ sequence induce significant migration of MDA-MB-231 cells. In particular, the observed migratory behavior suggests the presence of cell subpopulations associated with a {"}stationary{"} or a {"}migratory{"} phenotype, the latter determining a considerable cell migration at the sub-cm length scale. These findings are of great importance as they suggest for the first time an active role of biological surfaces exposing the IGD motif in the multicomponent orchestration of cellular signaling involved in the metastatic progression.",
keywords = "Cancer cells, Cell migration, Chemical gradients, IGD motifs, Peptide SAMs",
author = "Valentina Corvaglia and Riccardo Marega and {De Leo}, Federica and Carine Michiels and Davide Bonifazi",
note = "{\circledC} 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2016",
doi = "10.1002/smll.201501963",
language = "English",
volume = "12",
pages = "321--329",
journal = "Small",
issn = "1613-6810",
publisher = "Wiley-VCH Verlag",
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Unleashing Cancer Cells on Surfaces Exposing Motogenic IGDQ Peptides. / Corvaglia, Valentina; Marega, Riccardo; De Leo, Federica; Michiels, Carine; Bonifazi, Davide.

Dans: Small, Vol 12, Numéro 3, 2016, p. 321-329.

Résultats de recherche: Contribution à un journal/une revueArticle

TY - JOUR

T1 - Unleashing Cancer Cells on Surfaces Exposing Motogenic IGDQ Peptides

AU - Corvaglia, Valentina

AU - Marega, Riccardo

AU - De Leo, Federica

AU - Michiels, Carine

AU - Bonifazi, Davide

N1 - © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2016

Y1 - 2016

N2 - Thiolated peptides bearing the Ile-Gly-Asp (IGD) motif, a highly conserved sequence of fibronectin, are used for the preparation of anisotropic self-assembled monolayers (SAM gradients) to study the whole-population migratory behavior of metastatic breast cancer cells (MDA-MB-231 cells). Ile-Gly-Asp-Gln-(IGDQ)-exposing SAMs sustain the adhesion of MDA-MB-231 cells by triggering focal adhesion kinase phosphorylation, similarly to the analogous Gly-Arg-Gly-Asp-(GRGD)-terminating surfaces. However, the biological responses of different cell lines interfaced with the SAM gradients show that only those exposing the IGDQ sequence induce significant migration of MDA-MB-231 cells. In particular, the observed migratory behavior suggests the presence of cell subpopulations associated with a "stationary" or a "migratory" phenotype, the latter determining a considerable cell migration at the sub-cm length scale. These findings are of great importance as they suggest for the first time an active role of biological surfaces exposing the IGD motif in the multicomponent orchestration of cellular signaling involved in the metastatic progression.

AB - Thiolated peptides bearing the Ile-Gly-Asp (IGD) motif, a highly conserved sequence of fibronectin, are used for the preparation of anisotropic self-assembled monolayers (SAM gradients) to study the whole-population migratory behavior of metastatic breast cancer cells (MDA-MB-231 cells). Ile-Gly-Asp-Gln-(IGDQ)-exposing SAMs sustain the adhesion of MDA-MB-231 cells by triggering focal adhesion kinase phosphorylation, similarly to the analogous Gly-Arg-Gly-Asp-(GRGD)-terminating surfaces. However, the biological responses of different cell lines interfaced with the SAM gradients show that only those exposing the IGDQ sequence induce significant migration of MDA-MB-231 cells. In particular, the observed migratory behavior suggests the presence of cell subpopulations associated with a "stationary" or a "migratory" phenotype, the latter determining a considerable cell migration at the sub-cm length scale. These findings are of great importance as they suggest for the first time an active role of biological surfaces exposing the IGD motif in the multicomponent orchestration of cellular signaling involved in the metastatic progression.

KW - Cancer cells

KW - Cell migration

KW - Chemical gradients

KW - IGD motifs

KW - Peptide SAMs

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U2 - 10.1002/smll.201501963

DO - 10.1002/smll.201501963

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VL - 12

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