Unfolding IGDQ Peptides for Engineering Motogenic Interfaces

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

Extracellular matrix (ECM)-mimicking surfaces are pivotal tools in understanding adherent cell physiopathology. In this sense, we have recently reported on a discrete set of ECM-mimicking SAMs, among which only those exposing IGDQ peptide-alkanethiols sustain the adhesion of MDA-MB-231 cells by triggering FAK phosphorylation and peculiarly induce the migration of individual cancer cells on the subcentimeter scale. Starting from the experimentally observed relationship among the SAM composition, organization, and biological response, a systematic computational characterization aided in pinpointing the atomistic details through which specific composition and organization achieve the desired biological responsiveness. Specifically, the solvent, number and type of peptides, and presence or absence of surface fillers were accurately considered, creating representative model SAMs simulated by means of classical molecular dynamics (MD) with a view toward unravelling the experimental evidence, revealing how the conformational and structural features of these substrates dictate the specific motogenic responses. Through complementary experimental and computational investigations, it clearly emerges that there exists a distinct and precise mutual interaction among IGDQ-peptides, the surface fillers, and Au, which controls the structural properties of the ECM-mimicking SAMs and thus their motogenic potential.

langue originaleAnglais
Pages (de - à)7512-7528
Nombre de pages17
journalLangmuir
Volume33
Numéro de publication30
Les DOIs
étatPublié - 1 août 2017

Empreinte digitale

Peptides
peptides
engineering
fillers
Fillers
matrices
multiple docking adapters
phosphorylation
Phosphorylation
cells
Chemical analysis
Molecular dynamics
Structural properties
adhesion
Adhesion
cancer
Cells
molecular dynamics
Substrates
interactions

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title = "Unfolding IGDQ Peptides for Engineering Motogenic Interfaces",
abstract = "Extracellular matrix (ECM)-mimicking surfaces are pivotal tools in understanding adherent cell physiopathology. In this sense, we have recently reported on a discrete set of ECM-mimicking SAMs, among which only those exposing IGDQ peptide-alkanethiols sustain the adhesion of MDA-MB-231 cells by triggering FAK phosphorylation and peculiarly induce the migration of individual cancer cells on the subcentimeter scale. Starting from the experimentally observed relationship among the SAM composition, organization, and biological response, a systematic computational characterization aided in pinpointing the atomistic details through which specific composition and organization achieve the desired biological responsiveness. Specifically, the solvent, number and type of peptides, and presence or absence of surface fillers were accurately considered, creating representative model SAMs simulated by means of classical molecular dynamics (MD) with a view toward unravelling the experimental evidence, revealing how the conformational and structural features of these substrates dictate the specific motogenic responses. Through complementary experimental and computational investigations, it clearly emerges that there exists a distinct and precise mutual interaction among IGDQ-peptides, the surface fillers, and Au, which controls the structural properties of the ECM-mimicking SAMs and thus their motogenic potential.",
author = "{De Leo}, Federica and Riccardo Marega and Valentina Corvaglia and Rodolfo Tondo and {Lo Cicero}, Matteo and Simone Silvestrini and Davide Bonifazi",
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Unfolding IGDQ Peptides for Engineering Motogenic Interfaces. / De Leo, Federica; Marega, Riccardo; Corvaglia, Valentina; Tondo, Rodolfo; Lo Cicero, Matteo; Silvestrini, Simone; Bonifazi, Davide.

Dans: Langmuir, Vol 33, Numéro 30, 01.08.2017, p. 7512-7528.

Résultats de recherche: Contribution à un journal/une revueArticle

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