Unfolding IGDQ Peptides for Engineering Motogenic Interfaces

Federica De Leo, Riccardo Marega, Valentina Corvaglia, Rodolfo Tondo, Matteo Lo Cicero, Simone Silvestrini, Davide Bonifazi

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    Résumé

    Extracellular matrix (ECM)-mimicking surfaces are pivotal tools in understanding adherent cell physiopathology. In this sense, we have recently reported on a discrete set of ECM-mimicking SAMs, among which only those exposing IGDQ peptide-alkanethiols sustain the adhesion of MDA-MB-231 cells by triggering FAK phosphorylation and peculiarly induce the migration of individual cancer cells on the subcentimeter scale. Starting from the experimentally observed relationship among the SAM composition, organization, and biological response, a systematic computational characterization aided in pinpointing the atomistic details through which specific composition and organization achieve the desired biological responsiveness. Specifically, the solvent, number and type of peptides, and presence or absence of surface fillers were accurately considered, creating representative model SAMs simulated by means of classical molecular dynamics (MD) with a view toward unravelling the experimental evidence, revealing how the conformational and structural features of these substrates dictate the specific motogenic responses. Through complementary experimental and computational investigations, it clearly emerges that there exists a distinct and precise mutual interaction among IGDQ-peptides, the surface fillers, and Au, which controls the structural properties of the ECM-mimicking SAMs and thus their motogenic potential.

    langue originaleAnglais
    Pages (de - à)7512-7528
    Nombre de pages17
    journalLangmuir
    Volume33
    Numéro de publication30
    Les DOIs
    Etat de la publicationPublié - 1 août 2017

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