Translational profiling through biotinylation of tagged ribosomes in zebrafish

Michael P. Housley, Sven Reischauer, Marc Dieu, Martine Raes, Didier Y R Stainier, Benoit Vanhollebeke

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Heterogeneitywithin a population of cells of the sametype is a common theme in metazoan biology. Dissecting complex developmental and physiological processes crucially relies on our ability to probe the expression profile of these cell subpopulations. Current strategies rely on cell enrichment based on sequential or simultaneous use ofmultiple intersecting markers starting from a heterogeneous cell suspension. The extensive tissue manipulations required to generate single-cell suspensions, as well as the complexity of the required equipment, inherently complicate these approaches. Here, we propose an alternative methodology based on a genetically encoded system in the model organism Danio rerio (zebrafish). In transgenic fish, we take advantage of the combinatorial biotin transfer system, where polysome-associated mRNAs are selectively recovered from cells expressing both a tagged ribosomal subunit, Rpl10a, and the bacterial biotin ligase BirA. We have applied this technique to skeletal muscle development and identified new genes with interesting temporal expression patterns. Through this work we have thus developed additional tools for highly specific gene expression profiling.

langue originaleAnglais
Pages (de - à)3988-3993
Nombre de pages6
journalDevelopment (Cambridge)
Numéro de publication20
Les DOIs
Etat de la publicationPublié - 1 janv. 2014

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