Translational control of cell division by elongator

Fanelie Bauer, Akihisa Matsuyama, Julie Candiracci, Marc Dieu, Judith Scheliga, Dieter A Wolf, Minoru Yoshida, Damien Hermand

Résultats de recherche: Contribution à un journal/une revueArticle

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Résumé

Elongator is required for the synthesis of the mcm(5)s(2) modification found on tRNAs recognizing AA-ending codons. In order to obtain a global picture of the role of Elongator in translation, we used reverse protein arrays to screen the fission yeast proteome for translation defects. Unexpectedly, this revealed that Elongator inactivation mainly affected three specific functional groups including proteins implicated in cell division. The absence of Elongator results in a delay in mitosis onset and cytokinesis defects. We demonstrate that the kinase Cdr2, which is a central regulator of mitosis and cytokinesis, is under translational control by Elongator due to the Lysine codon usage bias of the cdr2 coding sequence. These findings uncover a mechanism by which the codon usage, coupled to tRNA modifications, fundamentally contributes to gene expression and cellular functions.
langue originaleAnglais
Pages (de - à)424-33
Nombre de pages10
journalCell Reports
Volume1
Numéro de publication5
Les DOIs
étatPublié - 2012

Empreinte digitale

Transfer RNA
Codon
Cell Division
Cytokinesis
Cells
Mitosis
Defects
Proteome
Gene expression
Yeast
Functional groups
Lysine
Protein Array Analysis
Proteins
Phosphotransferases
Schizosaccharomyces
Gene Expression

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Bauer, F., Matsuyama, A., Candiracci, J., Dieu, M., Scheliga, J., Wolf, D. A., ... Hermand, D. (2012). Translational control of cell division by elongator. Cell Reports, 1(5), 424-33. https://doi.org/10.1016/j.celrep.2012.04.001
Bauer, Fanelie ; Matsuyama, Akihisa ; Candiracci, Julie ; Dieu, Marc ; Scheliga, Judith ; Wolf, Dieter A ; Yoshida, Minoru ; Hermand, Damien. / Translational control of cell division by elongator. Dans: Cell Reports. 2012 ; Vol 1, Numéro 5. p. 424-33.
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abstract = "Elongator is required for the synthesis of the mcm(5)s(2) modification found on tRNAs recognizing AA-ending codons. In order to obtain a global picture of the role of Elongator in translation, we used reverse protein arrays to screen the fission yeast proteome for translation defects. Unexpectedly, this revealed that Elongator inactivation mainly affected three specific functional groups including proteins implicated in cell division. The absence of Elongator results in a delay in mitosis onset and cytokinesis defects. We demonstrate that the kinase Cdr2, which is a central regulator of mitosis and cytokinesis, is under translational control by Elongator due to the Lysine codon usage bias of the cdr2 coding sequence. These findings uncover a mechanism by which the codon usage, coupled to tRNA modifications, fundamentally contributes to gene expression and cellular functions.",
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Bauer, F, Matsuyama, A, Candiracci, J, Dieu, M, Scheliga, J, Wolf, DA, Yoshida, M & Hermand, D 2012, 'Translational control of cell division by elongator', Cell Reports, VOL. 1, Numéro 5, p. 424-33. https://doi.org/10.1016/j.celrep.2012.04.001

Translational control of cell division by elongator. / Bauer, Fanelie; Matsuyama, Akihisa; Candiracci, Julie; Dieu, Marc; Scheliga, Judith; Wolf, Dieter A; Yoshida, Minoru; Hermand, Damien.

Dans: Cell Reports, Vol 1, Numéro 5, 2012, p. 424-33.

Résultats de recherche: Contribution à un journal/une revueArticle

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T1 - Translational control of cell division by elongator

AU - Bauer, Fanelie

AU - Matsuyama, Akihisa

AU - Candiracci, Julie

AU - Dieu, Marc

AU - Scheliga, Judith

AU - Wolf, Dieter A

AU - Yoshida, Minoru

AU - Hermand, Damien

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AB - Elongator is required for the synthesis of the mcm(5)s(2) modification found on tRNAs recognizing AA-ending codons. In order to obtain a global picture of the role of Elongator in translation, we used reverse protein arrays to screen the fission yeast proteome for translation defects. Unexpectedly, this revealed that Elongator inactivation mainly affected three specific functional groups including proteins implicated in cell division. The absence of Elongator results in a delay in mitosis onset and cytokinesis defects. We demonstrate that the kinase Cdr2, which is a central regulator of mitosis and cytokinesis, is under translational control by Elongator due to the Lysine codon usage bias of the cdr2 coding sequence. These findings uncover a mechanism by which the codon usage, coupled to tRNA modifications, fundamentally contributes to gene expression and cellular functions.

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Bauer F, Matsuyama A, Candiracci J, Dieu M, Scheliga J, Wolf DA et al. Translational control of cell division by elongator. Cell Reports. 2012;1(5):424-33. https://doi.org/10.1016/j.celrep.2012.04.001