TY - JOUR
T1 - Tools for photomotor response assay standardization in ecotoxicological studies
T2 - Example of exposure to gentamicin in the freshwater planaria Schmidtea mediterranea
AU - Mathiron, Anthony G.E.
AU - Rejo, Lucia
AU - Chapeau, Florian
AU - Malgouyres, Jean Michel
AU - Silvestre, Frédéric
AU - Vignet, Caroline
N1 - Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/9
Y1 - 2023/9
N2 - Photomotor response assay (PMR) is very useful in an ecotoxicological context because it allows evaluation of behavioral response to potential toxic compounds. However, a lack of procedure standardization makes results comparison difficult between labs and organisms. Here, we aimed to propose five different tools to standardize the PMR procedure so that it may be applied to all model species, regarding: (1) the minimum total sample size, (2) the acclimation period, (3) the number and duration of light and dark phases alternation, (4) the measured behavior, and (5) the statistical analysis. As an example of procedure application, we analyzed the effect of an exposure to the antibiotic gentamicin on the locomotion behavior during PMR in an invertebrate species: the asexual freshwater planaria Schmidtea mediterranea. We encourage future studies using PMR to follow these five tools to improve data analysis and results comparability.
AB - Photomotor response assay (PMR) is very useful in an ecotoxicological context because it allows evaluation of behavioral response to potential toxic compounds. However, a lack of procedure standardization makes results comparison difficult between labs and organisms. Here, we aimed to propose five different tools to standardize the PMR procedure so that it may be applied to all model species, regarding: (1) the minimum total sample size, (2) the acclimation period, (3) the number and duration of light and dark phases alternation, (4) the measured behavior, and (5) the statistical analysis. As an example of procedure application, we analyzed the effect of an exposure to the antibiotic gentamicin on the locomotion behavior during PMR in an invertebrate species: the asexual freshwater planaria Schmidtea mediterranea. We encourage future studies using PMR to follow these five tools to improve data analysis and results comparability.
KW - Behavior
KW - Invertebrate
KW - Linear mixed models
KW - Locomotion
KW - Pharmaceuticals
KW - R software
UR - http://www.scopus.com/inward/record.url?scp=85170717186&partnerID=8YFLogxK
U2 - 10.1016/j.etap.2023.104242
DO - 10.1016/j.etap.2023.104242
M3 - Article
C2 - 37573897
AN - SCOPUS:85170717186
SN - 1382-6689
VL - 102
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
M1 - 104242
ER -