TMEM45A Is Dispensable for Epidermal Morphogenesis, Keratinization and Barrier Formation

Aurélie Hayez, Edith Roegiers, Jérémy Malaisse, Benoît Balau, Christiane Sterpin, Younes Achouri, Catherine Lambert De Rouvroit, Yves Poumay, Carine Michiels, Olivier De Backer

Résultats de recherche: Contribution à un journal/une revueArticle

31 Downloads (Pure)

Résumé

TMEM45A gene encodes an initially uncharacterized predicted transmembrane protein. We previously showed that this gene is highly expressed in keratinocytes where its expression correlates with keratinization, suggesting a role in normal epidermal physiology. To test this hypothesis, we generated TMEM45A knockout mice and found that these mice develop without any evident phenotype. The morphology of the epidermis assessed by histology and by labelling differentiation markers in immunofluorescence was not altered. Toluidine blue permeability assay showed that the epidermal barrier develops normally during embryonic development. We also showed that depletion of TMEM45A in human keratinocytes does not alter their potential to form in vitro 3D-reconstructed epidermis. Indeed, epidermis with normal morphogenesis were generated from TMEM45A-silenced keratinocytes. Their expression of differentiation markers quantified by RT-qPCR and evidenced by immunofluorescence labelling as well as their barrier function estimated by Lucifer yellow permeability were similar to the control epidermis. In summary, TMEM45A gene expression is dispensable for epidermal morphogenesis, keratinization and barrier formation. If this protein plays a role in the epidermis, its experimental depletion can possibly be compensated by other proteins in the two experimental models analyzed in this study.

langue originaleAnglais
Pages (de - à)e0147069
journalPLoS ONE
Volume11
Numéro de publication1
Les DOIs
étatPublié - 2016

Empreinte digitale

keratinization
epidermis (animal)
Morphogenesis
Epidermis
morphogenesis
Differentiation Antigens
keratinocytes
Labeling
Keratinocytes
Genes
Tolonium Chloride
Proteins
Histology
Physiology
fluorescent antibody technique
Fluorescent Antibody Technique
Permeability
permeability
Gene expression
Assays

Citer ceci

Hayez, Aurélie ; Roegiers, Edith ; Malaisse, Jérémy ; Balau, Benoît ; Sterpin, Christiane ; Achouri, Younes ; Lambert De Rouvroit, Catherine ; Poumay, Yves ; Michiels, Carine ; De Backer, Olivier. / TMEM45A Is Dispensable for Epidermal Morphogenesis, Keratinization and Barrier Formation. Dans: PLoS ONE. 2016 ; Vol 11, Numéro 1. p. e0147069.
@article{02c209d4da344e40a0db33637362a8fc,
title = "TMEM45A Is Dispensable for Epidermal Morphogenesis, Keratinization and Barrier Formation",
abstract = "TMEM45A gene encodes an initially uncharacterized predicted transmembrane protein. We previously showed that this gene is highly expressed in keratinocytes where its expression correlates with keratinization, suggesting a role in normal epidermal physiology. To test this hypothesis, we generated TMEM45A knockout mice and found that these mice develop without any evident phenotype. The morphology of the epidermis assessed by histology and by labelling differentiation markers in immunofluorescence was not altered. Toluidine blue permeability assay showed that the epidermal barrier develops normally during embryonic development. We also showed that depletion of TMEM45A in human keratinocytes does not alter their potential to form in vitro 3D-reconstructed epidermis. Indeed, epidermis with normal morphogenesis were generated from TMEM45A-silenced keratinocytes. Their expression of differentiation markers quantified by RT-qPCR and evidenced by immunofluorescence labelling as well as their barrier function estimated by Lucifer yellow permeability were similar to the control epidermis. In summary, TMEM45A gene expression is dispensable for epidermal morphogenesis, keratinization and barrier formation. If this protein plays a role in the epidermis, its experimental depletion can possibly be compensated by other proteins in the two experimental models analyzed in this study.",
author = "Aur{\'e}lie Hayez and Edith Roegiers and J{\'e}r{\'e}my Malaisse and Beno{\^i}t Balau and Christiane Sterpin and Younes Achouri and {Lambert De Rouvroit}, Catherine and Yves Poumay and Carine Michiels and {De Backer}, Olivier",
year = "2016",
doi = "10.1371/journal.pone.0147069",
language = "English",
volume = "11",
pages = "e0147069",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "1",

}

TMEM45A Is Dispensable for Epidermal Morphogenesis, Keratinization and Barrier Formation. / Hayez, Aurélie; Roegiers, Edith; Malaisse, Jérémy; Balau, Benoît; Sterpin, Christiane; Achouri, Younes; Lambert De Rouvroit, Catherine; Poumay, Yves; Michiels, Carine; De Backer, Olivier.

Dans: PLoS ONE, Vol 11, Numéro 1, 2016, p. e0147069.

Résultats de recherche: Contribution à un journal/une revueArticle

TY - JOUR

T1 - TMEM45A Is Dispensable for Epidermal Morphogenesis, Keratinization and Barrier Formation

AU - Hayez, Aurélie

AU - Roegiers, Edith

AU - Malaisse, Jérémy

AU - Balau, Benoît

AU - Sterpin, Christiane

AU - Achouri, Younes

AU - Lambert De Rouvroit, Catherine

AU - Poumay, Yves

AU - Michiels, Carine

AU - De Backer, Olivier

PY - 2016

Y1 - 2016

N2 - TMEM45A gene encodes an initially uncharacterized predicted transmembrane protein. We previously showed that this gene is highly expressed in keratinocytes where its expression correlates with keratinization, suggesting a role in normal epidermal physiology. To test this hypothesis, we generated TMEM45A knockout mice and found that these mice develop without any evident phenotype. The morphology of the epidermis assessed by histology and by labelling differentiation markers in immunofluorescence was not altered. Toluidine blue permeability assay showed that the epidermal barrier develops normally during embryonic development. We also showed that depletion of TMEM45A in human keratinocytes does not alter their potential to form in vitro 3D-reconstructed epidermis. Indeed, epidermis with normal morphogenesis were generated from TMEM45A-silenced keratinocytes. Their expression of differentiation markers quantified by RT-qPCR and evidenced by immunofluorescence labelling as well as their barrier function estimated by Lucifer yellow permeability were similar to the control epidermis. In summary, TMEM45A gene expression is dispensable for epidermal morphogenesis, keratinization and barrier formation. If this protein plays a role in the epidermis, its experimental depletion can possibly be compensated by other proteins in the two experimental models analyzed in this study.

AB - TMEM45A gene encodes an initially uncharacterized predicted transmembrane protein. We previously showed that this gene is highly expressed in keratinocytes where its expression correlates with keratinization, suggesting a role in normal epidermal physiology. To test this hypothesis, we generated TMEM45A knockout mice and found that these mice develop without any evident phenotype. The morphology of the epidermis assessed by histology and by labelling differentiation markers in immunofluorescence was not altered. Toluidine blue permeability assay showed that the epidermal barrier develops normally during embryonic development. We also showed that depletion of TMEM45A in human keratinocytes does not alter their potential to form in vitro 3D-reconstructed epidermis. Indeed, epidermis with normal morphogenesis were generated from TMEM45A-silenced keratinocytes. Their expression of differentiation markers quantified by RT-qPCR and evidenced by immunofluorescence labelling as well as their barrier function estimated by Lucifer yellow permeability were similar to the control epidermis. In summary, TMEM45A gene expression is dispensable for epidermal morphogenesis, keratinization and barrier formation. If this protein plays a role in the epidermis, its experimental depletion can possibly be compensated by other proteins in the two experimental models analyzed in this study.

U2 - 10.1371/journal.pone.0147069

DO - 10.1371/journal.pone.0147069

M3 - Article

C2 - 26785122

VL - 11

SP - e0147069

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 1

ER -