The role of thioredoxin reductase in gold nanoparticle radiosensitization effects

Sébastien Penninckx; Anne-Catherine Heuskin; Carine Michiels; Stéphane Lucas

doi:10.2217/nnm-2018-0171

The role of thioredoxin reductase in gold nanoparticle radiosensitization effects

Sébastien Penninckx, Anne-Catherine Heuskin, Carine Michiels, Stéphane Lucas

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

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Résumé

AIM: To identify new mechanisms responsible for the radiosensitization effect of gold nanoparticles (GNPs).

MATERIALS & METHODS: A549 lung carcinoma cells were incubated with 10-nm GNPs during 6 or 24 h before to be exposed to 25 keV/μm protons or 225 kV x-rays.

RESULTS: GNP incubation led to a time-dependent mitochondria membrane depolarization, oxidative stress and to x-ray and proton radiosensitization. Moreover, a marked inhibition of thioredoxin reductase was observed. Irradiation of cells invalidated for thioredoxin reductase evidenced a radiosensitization effect, suggesting that this enzyme is a potential GNP target.

CONCLUSION: We suggest that GNPs play a radiosensitizer role by weakening detoxification systems. Altogether, these results open up promising novel strategies for the development of nanotechnologies associated to radiotherapy.

langue originale	Anglais
Pages (de - à)	2917-2937
Nombre de pages	21
journal	Nanomedicine
Volume	13
Numéro de publication	22
Les DOIs	https://doi.org/10.2217/nnm-2018-0171
Etat de la publication	Publié - 1 nov. 2018

SDG des Nations Unies

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Advances in the Mechanistic Understanding of Iron Oxide Nanoparticles' Radiosensitizing Properties
Ternad, I., Penninckx, S., Lecomte, V., Vangijzegem, T., Conrard, L., Lucas, S., Heuskin, A-C., Michiels, C., Muller, R. N., Stanicki, D. & Laurent, S., 2 janv. 2023, Dans: Nanomaterials. 13, 1, 201.
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PROTHER-WAL: Proton Therapy Research in Wallonia
MICHIELS, C., Lucas, S., Heuskin, A., Golli, E., Coos, R., Cini, N., Penninckx, S., Cardinal, M. & Henrotin, S.
1/01/19 → 31/07/26
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Charles Nicaise (!!Manager) & Francesca Cecchet (!!Manager)
Plateforme technologique Morphologie, imagerie
Equipement/installations: Equipement
Morphologie - Imagerie
Charles Nicaise (!!Manager) & Francesca Cecchet (!!Manager)
Plateforme technologique Morphologie, imagerie
Equipement/installations: Plateforme technolgique
Synthèse, Irradiation et Analyse de Matériaux (SIAM) (2016 - ...)
Pierre Louette (!!Manager), Julien Colaux (!!Manager), Alexandre Felten (!!Manager) & Jorge Humberto Mejia Mendoza (!!Manager)
Plateforme technologique Synthese, irradiation et analyse des materiaux
Equipement/installations: Plateforme technolgique

Contient cette citation

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title = "The role of thioredoxin reductase in gold nanoparticle radiosensitization effects",

abstract = "AIM: To identify new mechanisms responsible for the radiosensitization effect of gold nanoparticles (GNPs).MATERIALS & METHODS: A549 lung carcinoma cells were incubated with 10-nm GNPs during 6 or 24 h before to be exposed to 25 keV/μm protons or 225 kV x-rays.RESULTS: GNP incubation led to a time-dependent mitochondria membrane depolarization, oxidative stress and to x-ray and proton radiosensitization. Moreover, a marked inhibition of thioredoxin reductase was observed. Irradiation of cells invalidated for thioredoxin reductase evidenced a radiosensitization effect, suggesting that this enzyme is a potential GNP target.CONCLUSION: We suggest that GNPs play a radiosensitizer role by weakening detoxification systems. Altogether, these results open up promising novel strategies for the development of nanotechnologies associated to radiotherapy.",

keywords = "biological mechanism, gold nanoparticles, proton irradiation, radiosensitization, thioredoxin reductase, Protons, A549 Cells, Radiation-Sensitizing Agents/metabolism, Humans, Metal Nanoparticles/chemistry, Gold/chemistry, Oxidative Stress/drug effects, Particle Size, X-Rays, Surface Properties, DNA Damage/drug effects, Cell Proliferation/drug effects, Radiotherapy/methods, Thioredoxin-Disulfide Reductase/metabolism",

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year = "2018",

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doi = "10.2217/nnm-2018-0171",

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AU - Penninckx, Sébastien

AU - Heuskin, Anne-Catherine

AU - Michiels, Carine

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N2 - AIM: To identify new mechanisms responsible for the radiosensitization effect of gold nanoparticles (GNPs).MATERIALS & METHODS: A549 lung carcinoma cells were incubated with 10-nm GNPs during 6 or 24 h before to be exposed to 25 keV/μm protons or 225 kV x-rays.RESULTS: GNP incubation led to a time-dependent mitochondria membrane depolarization, oxidative stress and to x-ray and proton radiosensitization. Moreover, a marked inhibition of thioredoxin reductase was observed. Irradiation of cells invalidated for thioredoxin reductase evidenced a radiosensitization effect, suggesting that this enzyme is a potential GNP target.CONCLUSION: We suggest that GNPs play a radiosensitizer role by weakening detoxification systems. Altogether, these results open up promising novel strategies for the development of nanotechnologies associated to radiotherapy.

AB - AIM: To identify new mechanisms responsible for the radiosensitization effect of gold nanoparticles (GNPs).MATERIALS & METHODS: A549 lung carcinoma cells were incubated with 10-nm GNPs during 6 or 24 h before to be exposed to 25 keV/μm protons or 225 kV x-rays.RESULTS: GNP incubation led to a time-dependent mitochondria membrane depolarization, oxidative stress and to x-ray and proton radiosensitization. Moreover, a marked inhibition of thioredoxin reductase was observed. Irradiation of cells invalidated for thioredoxin reductase evidenced a radiosensitization effect, suggesting that this enzyme is a potential GNP target.CONCLUSION: We suggest that GNPs play a radiosensitizer role by weakening detoxification systems. Altogether, these results open up promising novel strategies for the development of nanotechnologies associated to radiotherapy.

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The role of thioredoxin reductase in gold nanoparticle radiosensitization effects

Résumé

SDG des Nations Unies

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Résultat de recherche

Advances in the Mechanistic Understanding of Iron Oxide Nanoparticles' Radiosensitizing Properties

Projets

PROTHER-WAL: Proton Therapy Research in Wallonia

Équipement

FACS

Morphologie - Imagerie

Synthèse, Irradiation et Analyse de Matériaux (SIAM) (2016 - ...)

Contient cette citation