Résumé
AIM: To identify new mechanisms responsible for the radiosensitization effect of gold nanoparticles (GNPs).
MATERIALS & METHODS: A549 lung carcinoma cells were incubated with 10-nm GNPs during 6 or 24 h before to be exposed to 25 keV/μm protons or 225 kV x-rays.
RESULTS: GNP incubation led to a time-dependent mitochondria membrane depolarization, oxidative stress and to x-ray and proton radiosensitization. Moreover, a marked inhibition of thioredoxin reductase was observed. Irradiation of cells invalidated for thioredoxin reductase evidenced a radiosensitization effect, suggesting that this enzyme is a potential GNP target.
CONCLUSION: We suggest that GNPs play a radiosensitizer role by weakening detoxification systems. Altogether, these results open up promising novel strategies for the development of nanotechnologies associated to radiotherapy.
langue originale | Anglais |
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Pages (de - à) | 2917-2937 |
Nombre de pages | 21 |
journal | Nanomedicine |
Volume | 13 |
Numéro de publication | 22 |
Date de mise en ligne précoce | 2018 |
Les DOIs | |
Etat de la publication | Publié - 1 nov. 2018 |
Modification externe | Oui |
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Équipement
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