TY - JOUR
T1 - The purification of plasma membranes from WI-38 fibroblasts. Effects of ageing on their composition
AU - Raes, Martine
AU - Houbion, A.
AU - Remacle, J.
N1 - Medline is the source for the MeSH terms of this document.
PY - 1981/1/1
Y1 - 1981/1/1
N2 - A three-step method for the purification of plasma membranes from WI-38 fibroblasts was developed thus allowing the recovery of 36-44% of the plasma membrane. Except in the case of galactosyltransferase, the activity of the contaminating enzymes was very low. Morphological observations confirm the presence of a homogeneous population of vesicles. Preparations obtained from young and old cell cultures were compared for their enzymatic and protein contents. With ageing the activity of 5'-nucleotidase significantly increases whereas that of alkaline phosphodiesterase I decreases. Out of the 26 components detected after sodium dodecyl sulphate polyacrylamide gel electrophoresis, four decreased but only one increased. Cellular ageing seems to fulfil a specific and localized effect on the plasma membrane.
AB - A three-step method for the purification of plasma membranes from WI-38 fibroblasts was developed thus allowing the recovery of 36-44% of the plasma membrane. Except in the case of galactosyltransferase, the activity of the contaminating enzymes was very low. Morphological observations confirm the presence of a homogeneous population of vesicles. Preparations obtained from young and old cell cultures were compared for their enzymatic and protein contents. With ageing the activity of 5'-nucleotidase significantly increases whereas that of alkaline phosphodiesterase I decreases. Out of the 26 components detected after sodium dodecyl sulphate polyacrylamide gel electrophoresis, four decreased but only one increased. Cellular ageing seems to fulfil a specific and localized effect on the plasma membrane.
UR - http://www.scopus.com/inward/record.url?scp=0019519891&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(81)90448-X
DO - 10.1016/0005-2736(81)90448-X
M3 - Article
AN - SCOPUS:0019519891
SN - 0005-2736
VL - 642
SP - 313
EP - 324
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 2
ER -