The in vitro antitumor activity of vitamins C and K3 against ovarian carcinoma

Vivian E. Von Gruenigen; James M. Jamison; Jacques Gilloteaux; Heather E. Lorimer; Marcia Summers; Robert R. Pollard; Carley A. Gwin; Jack L Summers

The in vitro antitumor activity of vitamins C and K₃ against ovarian carcinoma

Vivian E. Von Gruenigen, James M. Jamison, Jacques Gilloteaux, Heather E. Lorimer, Marcia Summers, Robert R. Pollard, Carley A. Gwin, Jack L Summers

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

Résumé

Background: The objective was to evaluate the cytotoxic effect and mechanism of action of vitamins C (VC) and K₃ (VK₃) on ovarian carcinoma. Materials and Methods: Cytotoxicity assays were performed on ovarian cancer cell lines with VC, VK₃ or a VC/VK₃ combination. FIC index was employed to evaluate synergism. Flow cytometry was accomplished at 90% cytotoxic doses. Light, transmission electron microscopy and DNA isolation were performed. Results: Antitumor activity was exhibited by both VC, VK₃ and VC/VK₃ demonstrated synergistic activity. VC/VK₃ may induce a G₁, block in the cell cycle. Combined vitamin treatment resulted in cells that maintain apparently intact nuclei while extruding pieces of organelle-free cytoplasm. Degradation of chromosomal DNA was observed. Conclusion: Cell death (autoschizis) displayed characteristics of both apoptosis and necrosis. The cytotoxic effects observed may enable vitamins C and K₃ to play an adjuvant role in the treatment of ovarian cancer.

langue originale	Anglais
Pages (de - à)	3279-3287
Nombre de pages	9
journal	Anticancer Research
Volume	23
Numéro de publication	4
Etat de la publication	Publié - juil. 2003
Modification externe	Oui

SDG des Nations Unies

Ce résultat contribue à ou aux Objectifs de développement durable suivants

Autres fichiers et liens

Link to publication in Scopus

Contient cette citation

@article{da2ffab4bca8430f80f365250fcd2e59,

title = "The in vitro antitumor activity of vitamins C and K3 against ovarian carcinoma",

abstract = "Background: The objective was to evaluate the cytotoxic effect and mechanism of action of vitamins C (VC) and K3 (VK3) on ovarian carcinoma. Materials and Methods: Cytotoxicity assays were performed on ovarian cancer cell lines with VC, VK3 or a VC/VK3 combination. FIC index was employed to evaluate synergism. Flow cytometry was accomplished at 90% cytotoxic doses. Light, transmission electron microscopy and DNA isolation were performed. Results: Antitumor activity was exhibited by both VC, VK3 and VC/VK3 demonstrated synergistic activity. VC/VK3 may induce a G1, block in the cell cycle. Combined vitamin treatment resulted in cells that maintain apparently intact nuclei while extruding pieces of organelle-free cytoplasm. Degradation of chromosomal DNA was observed. Conclusion: Cell death (autoschizis) displayed characteristics of both apoptosis and necrosis. The cytotoxic effects observed may enable vitamins C and K3 to play an adjuvant role in the treatment of ovarian cancer.",

keywords = "Autoschizis, Ovarian cancer, Vitamin C, Vitamin K",

author = "{Von Gruenigen}, {Vivian E.} and Jamison, {James M.} and Jacques Gilloteaux and Lorimer, {Heather E.} and Marcia Summers and Pollard, {Robert R.} and Gwin, {Carley A.} and Summers, {Jack L}",

year = "2003",

month = jul,

language = "English",

volume = "23",

pages = "3279--3287",

journal = "Anticancer Research",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

number = "4",

}

TY - JOUR

T1 - The in vitro antitumor activity of vitamins C and K3 against ovarian carcinoma

AU - Von Gruenigen, Vivian E.

AU - Jamison, James M.

AU - Gilloteaux, Jacques

AU - Lorimer, Heather E.

AU - Summers, Marcia

AU - Pollard, Robert R.

AU - Gwin, Carley A.

AU - Summers, Jack L

PY - 2003/7

Y1 - 2003/7

N2 - Background: The objective was to evaluate the cytotoxic effect and mechanism of action of vitamins C (VC) and K3 (VK3) on ovarian carcinoma. Materials and Methods: Cytotoxicity assays were performed on ovarian cancer cell lines with VC, VK3 or a VC/VK3 combination. FIC index was employed to evaluate synergism. Flow cytometry was accomplished at 90% cytotoxic doses. Light, transmission electron microscopy and DNA isolation were performed. Results: Antitumor activity was exhibited by both VC, VK3 and VC/VK3 demonstrated synergistic activity. VC/VK3 may induce a G1, block in the cell cycle. Combined vitamin treatment resulted in cells that maintain apparently intact nuclei while extruding pieces of organelle-free cytoplasm. Degradation of chromosomal DNA was observed. Conclusion: Cell death (autoschizis) displayed characteristics of both apoptosis and necrosis. The cytotoxic effects observed may enable vitamins C and K3 to play an adjuvant role in the treatment of ovarian cancer.

AB - Background: The objective was to evaluate the cytotoxic effect and mechanism of action of vitamins C (VC) and K3 (VK3) on ovarian carcinoma. Materials and Methods: Cytotoxicity assays were performed on ovarian cancer cell lines with VC, VK3 or a VC/VK3 combination. FIC index was employed to evaluate synergism. Flow cytometry was accomplished at 90% cytotoxic doses. Light, transmission electron microscopy and DNA isolation were performed. Results: Antitumor activity was exhibited by both VC, VK3 and VC/VK3 demonstrated synergistic activity. VC/VK3 may induce a G1, block in the cell cycle. Combined vitamin treatment resulted in cells that maintain apparently intact nuclei while extruding pieces of organelle-free cytoplasm. Degradation of chromosomal DNA was observed. Conclusion: Cell death (autoschizis) displayed characteristics of both apoptosis and necrosis. The cytotoxic effects observed may enable vitamins C and K3 to play an adjuvant role in the treatment of ovarian cancer.

KW - Autoschizis

KW - Ovarian cancer

KW - Vitamin C

KW - Vitamin K

UR - http://www.scopus.com/inward/record.url?scp=0041528197&partnerID=8YFLogxK

M3 - Article

C2 - 12926064

AN - SCOPUS:0041528197

SN - 0250-7005

VL - 23

SP - 3279

EP - 3287

JO - Anticancer Research

JF - Anticancer Research

IS - 4

ER -