Target immunity of the Tn3-family transposon Tn4430 requires specific interactions between the transposase and the terminal inverted repeats of the transposon

Emilien Nicolas, Michaël Lambin, Bernard Hallet

Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs

Résumé

Specificity of the Tn4430 target immunity signal was examined by fusing the transposase TnpA to the LacI repressor of Escherichia coli. The resulting chimeric proteins failed to impose immunity to DNA targets carrying copies of the lacO operator, though they were proficient in lacO binding in vivo and remained responsive to wild-type immunity conferred by the Tn4430 inverted repeat end. Intriguingly, the presence of lacO repeats within the target was found to strongly influence target site selection by Tn4430, but in a LacI-independent manner.

langue originaleAnglais
Pages (de - à)4233-8
Nombre de pages6
journalJournal of Bacteriology
Volume192
Numéro de publication16
Les DOIs
Etat de la publicationPublié - 2010
Modification externeOui

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