Résumé
Specificity of the Tn4430 target immunity signal was examined by fusing the transposase TnpA to the LacI repressor of Escherichia coli. The resulting chimeric proteins failed to impose immunity to DNA targets carrying copies of the lacO operator, though they were proficient in lacO binding in vivo and remained responsive to wild-type immunity conferred by the Tn4430 inverted repeat end. Intriguingly, the presence of lacO repeats within the target was found to strongly influence target site selection by Tn4430, but in a LacI-independent manner.
langue originale | Anglais |
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Pages (de - à) | 4233-8 |
Nombre de pages | 6 |
journal | Journal of Bacteriology |
Volume | 192 |
Numéro de publication | 16 |
Les DOIs | |
Etat de la publication | Publié - 2010 |
Modification externe | Oui |