Single nucleotide polymorphism genotyping and distribution of Coxiella burnetii strains from field samples in Belgium

Fabiana Dal Pozzo, Bénédicte Renaville, Ludovic Martinelle, Robert Renaville, Christine Thys, François Smeets, Nathalie Kirschvink, Fabien Grégoire, Laurent Delooz, Guy Czaplicki, Claude Saegerman

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

The genotypic characterization of Coxiella burnetii provides useful information about the strains circulating at the farm, region, or country level and may be used to identify the source of infection for animals and humans. The aim of the present study was to investigate the strains of C. burnetii circulating in caprine and bovine Belgian farms using a single nucleotide polymorphism (SNP) technique. Direct genotyping was applied to different samples (bulk tank milk, individual milk, vaginal swab, fetal product, and air sample). Besides the well-known SNP genotypes, unreported ones were found in bovine and caprine samples, increasing the variability of the strains found in the two species in Belgium. Moreover, multiple genotypes were detected contemporarily in caprine farms at different years of sampling and by using different samples. Interestingly, certain SNP genotypes were detected in both bovine and caprine samples, raising the question of interspecies transmission of the pathogen.

langueAnglais
Pages81-86
Nombre de pages6
journalApplied and Environmental Microbiology
Volume82
Numéro1
Les DOIs
étatPublié - 2016

Empreinte digitale

Coxiella burnetii
Belgium
genotyping
single nucleotide polymorphism
Single Nucleotide Polymorphism
polymorphism
genotype
Genotype
farm
milk
Milk
goats
Infectious Disease Transmission
sampling
farms
cattle
pathogen
Air
milk tanks
animal

Citer ceci

Pozzo, Fabiana Dal ; Renaville, Bénédicte ; Martinelle, Ludovic ; Renaville, Robert ; Thys, Christine ; Smeets, François ; Kirschvink, Nathalie ; Grégoire, Fabien ; Delooz, Laurent ; Czaplicki, Guy ; Saegerman, Claude. / Single nucleotide polymorphism genotyping and distribution of Coxiella burnetii strains from field samples in Belgium. Dans: Applied and Environmental Microbiology. 2016 ; Vol 82, Numéro 1. p. 81-86
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abstract = "The genotypic characterization of Coxiella burnetii provides useful information about the strains circulating at the farm, region, or country level and may be used to identify the source of infection for animals and humans. The aim of the present study was to investigate the strains of C. burnetii circulating in caprine and bovine Belgian farms using a single nucleotide polymorphism (SNP) technique. Direct genotyping was applied to different samples (bulk tank milk, individual milk, vaginal swab, fetal product, and air sample). Besides the well-known SNP genotypes, unreported ones were found in bovine and caprine samples, increasing the variability of the strains found in the two species in Belgium. Moreover, multiple genotypes were detected contemporarily in caprine farms at different years of sampling and by using different samples. Interestingly, certain SNP genotypes were detected in both bovine and caprine samples, raising the question of interspecies transmission of the pathogen.",
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Pozzo, FD, Renaville, B, Martinelle, L, Renaville, R, Thys, C, Smeets, F, Kirschvink, N, Grégoire, F, Delooz, L, Czaplicki, G & Saegerman, C 2016, 'Single nucleotide polymorphism genotyping and distribution of Coxiella burnetii strains from field samples in Belgium' Applied and Environmental Microbiology, VOL. 82, Numéro 1, p. 81-86. DOI: 10.1128/AEM.02799-15

Single nucleotide polymorphism genotyping and distribution of Coxiella burnetii strains from field samples in Belgium. / Pozzo, Fabiana Dal; Renaville, Bénédicte; Martinelle, Ludovic; Renaville, Robert; Thys, Christine; Smeets, François; Kirschvink, Nathalie; Grégoire, Fabien; Delooz, Laurent; Czaplicki, Guy; Saegerman, Claude.

Dans: Applied and Environmental Microbiology, Vol 82, Numéro 1, 2016, p. 81-86.

Résultats de recherche: Contribution à un journal/une revueArticle

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AU - Martinelle,Ludovic

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AU - Thys,Christine

AU - Smeets,François

AU - Kirschvink,Nathalie

AU - Grégoire,Fabien

AU - Delooz,Laurent

AU - Czaplicki,Guy

AU - Saegerman,Claude

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Pozzo FD, Renaville B, Martinelle L, Renaville R, Thys C, Smeets F et al. Single nucleotide polymorphism genotyping and distribution of Coxiella burnetii strains from field samples in Belgium. Applied and Environmental Microbiology. 2016;82(1):81-86. Disponible �, DOI: 10.1128/AEM.02799-15