TY - JOUR
T1 - Single nucleotide polymorphism genotyping and distribution of Coxiella burnetii strains from field samples in Belgium
AU - Pozzo, Fabiana Dal
AU - Renaville, Bénédicte
AU - Martinelle, Ludovic
AU - Renaville, Robert
AU - Thys, Christine
AU - Smeets, François
AU - Kirschvink, Nathalie
AU - Grégoire, Fabien
AU - Delooz, Laurent
AU - Czaplicki, Guy
AU - Saegerman, Claude
PY - 2016
Y1 - 2016
N2 - The genotypic characterization of Coxiella burnetii provides useful information about the strains circulating at the farm, region, or country level and may be used to identify the source of infection for animals and humans. The aim of the present study was to investigate the strains of C. burnetii circulating in caprine and bovine Belgian farms using a single nucleotide polymorphism (SNP) technique. Direct genotyping was applied to different samples (bulk tank milk, individual milk, vaginal swab, fetal product, and air sample). Besides the well-known SNP genotypes, unreported ones were found in bovine and caprine samples, increasing the variability of the strains found in the two species in Belgium. Moreover, multiple genotypes were detected contemporarily in caprine farms at different years of sampling and by using different samples. Interestingly, certain SNP genotypes were detected in both bovine and caprine samples, raising the question of interspecies transmission of the pathogen.
AB - The genotypic characterization of Coxiella burnetii provides useful information about the strains circulating at the farm, region, or country level and may be used to identify the source of infection for animals and humans. The aim of the present study was to investigate the strains of C. burnetii circulating in caprine and bovine Belgian farms using a single nucleotide polymorphism (SNP) technique. Direct genotyping was applied to different samples (bulk tank milk, individual milk, vaginal swab, fetal product, and air sample). Besides the well-known SNP genotypes, unreported ones were found in bovine and caprine samples, increasing the variability of the strains found in the two species in Belgium. Moreover, multiple genotypes were detected contemporarily in caprine farms at different years of sampling and by using different samples. Interestingly, certain SNP genotypes were detected in both bovine and caprine samples, raising the question of interspecies transmission of the pathogen.
UR - http://www.scopus.com/inward/record.url?scp=84953898037&partnerID=8YFLogxK
U2 - 10.1128/AEM.02799-15
DO - 10.1128/AEM.02799-15
M3 - Article
AN - SCOPUS:84953898037
SN - 0099-2240
VL - 82
SP - 81
EP - 86
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 1
ER -