Scarless excision of an insertion sequence restores capsule production and virulence in Acinetobacter baumannii

Clémence Whiteway; Adam Valcek; Chantal Philippe; Mojca Strazisar; Tim De Pooter; Ivan Mateus; Anke Breine; Charles Van der Henst

doi:10.1038/s41396-021-01179-3

Scarless excision of an insertion sequence restores capsule production and virulence in Acinetobacter baumannii

Clémence Whiteway
, Adam Valcek
, Chantal Philippe
, Mojca Strazisar
, Tim De Pooter
, Ivan Mateus
, Anke Breine
, Charles Van der Henst

Unite de recherche en biologie des micro-organismes

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

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Résumé

We identify a new mechanism mediating capsule production and virulence in the WHO and CDC priority ESKAPE pathogen Acinetobacter baumannii. Non-capsulated and avirulent bacteria can revert into a capsulated and virulent state upon scarless excision of an ISAba13 insertion sequence under stress conditions. Reversion events fully restore capsule production and in vivo virulence. This increases our knowledge about A. baumannii genome dynamics, and the regulation of capsule production, virulence and resistance.

langue originale	Anglais
Pages (de - à)	1473-1477
Nombre de pages	5
journal	ISME Journal
Volume	16
Numéro de publication	5
Les DOIs	https://doi.org/10.1038/s41396-021-01179-3
Etat de la publication	Publié - mai 2022

Financement

This project was supported by the Flanders Institute for Biotechnology (VIB) and has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 748032 (CV). AB is recipient of a PhD fellowship Strategic Basic Research of the Research Foundation – Flanders (FWO, File number: 77258).

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10.1038/s41396-021-01179-3

s41396-021-01179-3Version finale publiée, 1,19 MBLicense: CC BY

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title = "Scarless excision of an insertion sequence restores capsule production and virulence in Acinetobacter baumannii",

abstract = "We identify a new mechanism mediating capsule production and virulence in the WHO and CDC priority ESKAPE pathogen Acinetobacter baumannii. Non-capsulated and avirulent bacteria can revert into a capsulated and virulent state upon scarless excision of an ISAba13 insertion sequence under stress conditions. Reversion events fully restore capsule production and in vivo virulence. This increases our knowledge about A. baumannii genome dynamics, and the regulation of capsule production, virulence and resistance.",

author = "Cl{\'e}mence Whiteway and Adam Valcek and Chantal Philippe and Mojca Strazisar and \{De Pooter\}, Tim and Ivan Mateus and Anke Breine and \{Van der Henst\}, Charles",

note = "Funding Information: This project was supported by the Flanders Institute for Biotechnology (VIB) and has received funding from the European Union{\textquoteright}s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 748032 (CV). AB is recipient of a PhD fellowship Strategic Basic Research of the Research Foundation – Flanders (FWO, File number: 77258). Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = may,

doi = "10.1038/s41396-021-01179-3",

language = "English",

volume = "16",

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TY - JOUR

T1 - Scarless excision of an insertion sequence restores capsule production and virulence in Acinetobacter baumannii

AU - Whiteway, Clémence

AU - Valcek, Adam

AU - Philippe, Chantal

AU - Strazisar, Mojca

AU - De Pooter, Tim

AU - Mateus, Ivan

AU - Breine, Anke

AU - Van der Henst, Charles

N1 - Funding Information: This project was supported by the Flanders Institute for Biotechnology (VIB) and has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 748032 (CV). AB is recipient of a PhD fellowship Strategic Basic Research of the Research Foundation – Flanders (FWO, File number: 77258). Publisher Copyright: © 2022, The Author(s).

PY - 2022/5

Y1 - 2022/5

N2 - We identify a new mechanism mediating capsule production and virulence in the WHO and CDC priority ESKAPE pathogen Acinetobacter baumannii. Non-capsulated and avirulent bacteria can revert into a capsulated and virulent state upon scarless excision of an ISAba13 insertion sequence under stress conditions. Reversion events fully restore capsule production and in vivo virulence. This increases our knowledge about A. baumannii genome dynamics, and the regulation of capsule production, virulence and resistance.

AB - We identify a new mechanism mediating capsule production and virulence in the WHO and CDC priority ESKAPE pathogen Acinetobacter baumannii. Non-capsulated and avirulent bacteria can revert into a capsulated and virulent state upon scarless excision of an ISAba13 insertion sequence under stress conditions. Reversion events fully restore capsule production and in vivo virulence. This increases our knowledge about A. baumannii genome dynamics, and the regulation of capsule production, virulence and resistance.

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DO - 10.1038/s41396-021-01179-3

M3 - Article

C2 - 34949784

AN - SCOPUS:85121601325

SN - 1751-7362

VL - 16

SP - 1473

EP - 1477

JO - ISME Journal

JF - ISME Journal

IS - 5

ER -

Scarless excision of an insertion sequence restores capsule production and virulence in Acinetobacter baumannii

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