Salinity stress, enhancing basal and induced immune responses in striped catfish Pangasianodon hypophthalmus (Sauvage)

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

In the Mekong Delta, striped catfish are faced with chronic salinity stress related to saltwater intrusion induced by global climatic changes. In this study, striped catfish juveniles were submitted to a prolonged salinity stress (up to 10 ppt) over three weeks followed by infection with a virulent bacterial strain, Edwardsiella ictaluri. Osmoregulatory parameters were investigated. In addition, a label free quantitative proteomics workflow was performed on kidneys. The workflow consisted of an initial global profiling of relative peptide abundances (by LC/MS, peak area quantification based on extracted ion currents), followed by identification (by MS/MS). The aim of the study was to highlight specific functional pathways modified during realistic salinity stress, particularly those involved in immunity. In kidney proteome, 2483 proteins were identified, of which 400 proteins were differentially expressed between the freshwater and the saline water conditions. Several pathways and functional categories were highlighted, mostly related to energy metabolism, protein metabolism, actin cytoskeleton, signaling, immunity, and detoxification. In particular, the responsiveness of proteins involved in small GTPases and Mitogen Activated Protein Kinase p38 signaling, phagolysosome maturation, and T-cells regulation is discussed. Statement of significance In the Mekong River Delta (Vietnam), striped catfish production is threatened by extensive sea water intrusion exacerbated by sea level rise. In fish, the effect of chronic exposure to salinity stress on immune capacities and response to disease has been poorly investigated. This study aims to highlight the main molecular changes occurring in the kidney during acclimation to salinity stress, particularly those involved in the immune defences of fish.

langueAnglais
Pages12-24
Nombre de pages13
journalJournal of Proteomics
Volume167
Les DOIs
étatPublié - 7 sept. 2017

Empreinte digitale

Catfishes
Salinity
Fish
Workflow
Proteins
Kidney
Edwardsiella ictaluri
Immunity
Fishes
Detoxification
Monomeric GTP-Binding Proteins
T-cells
Sea level
p38 Mitogen-Activated Protein Kinases
Proteome
Phagosomes
Metabolism
Vietnam
Acclimatization
Seawater

mots-clés

    Citer ceci

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    title = "Salinity stress, enhancing basal and induced immune responses in striped catfish Pangasianodon hypophthalmus (Sauvage)",
    abstract = "In the Mekong Delta, striped catfish are faced with chronic salinity stress related to saltwater intrusion induced by global climatic changes. In this study, striped catfish juveniles were submitted to a prolonged salinity stress (up to 10 ppt) over three weeks followed by infection with a virulent bacterial strain, Edwardsiella ictaluri. Osmoregulatory parameters were investigated. In addition, a label free quantitative proteomics workflow was performed on kidneys. The workflow consisted of an initial global profiling of relative peptide abundances (by LC/MS, peak area quantification based on extracted ion currents), followed by identification (by MS/MS). The aim of the study was to highlight specific functional pathways modified during realistic salinity stress, particularly those involved in immunity. In kidney proteome, 2483 proteins were identified, of which 400 proteins were differentially expressed between the freshwater and the saline water conditions. Several pathways and functional categories were highlighted, mostly related to energy metabolism, protein metabolism, actin cytoskeleton, signaling, immunity, and detoxification. In particular, the responsiveness of proteins involved in small GTPases and Mitogen Activated Protein Kinase p38 signaling, phagolysosome maturation, and T-cells regulation is discussed. Statement of significance In the Mekong River Delta (Vietnam), striped catfish production is threatened by extensive sea water intrusion exacerbated by sea level rise. In fish, the effect of chronic exposure to salinity stress on immune capacities and response to disease has been poorly investigated. This study aims to highlight the main molecular changes occurring in the kidney during acclimation to salinity stress, particularly those involved in the immune defences of fish.",
    keywords = "Catfish, Immunity, Infectious disease, Label-free proteomics, Osmoregulation",
    author = "M{\'e}lodie Schmitz and Tamar Ziv and Arie Admon and S{\'e}bastien Baekelandt and Mandiki, {Syaghalirwa N.M.} and Ma{\"e}lenn L'Hoir and Patrick Kestemont",
    year = "2017",
    month = "9",
    day = "7",
    doi = "10.1016/j.jprot.2017.08.005",
    language = "English",
    volume = "167",
    pages = "12--24",
    journal = "Journal of Proteomics",
    issn = "1874-3919",
    publisher = "Elsevier",

    }

    TY - JOUR

    T1 - Salinity stress, enhancing basal and induced immune responses in striped catfish Pangasianodon hypophthalmus (Sauvage)

    AU - Schmitz,Mélodie

    AU - Ziv,Tamar

    AU - Admon,Arie

    AU - Baekelandt,Sébastien

    AU - Mandiki,Syaghalirwa N.M.

    AU - L'Hoir,Maëlenn

    AU - Kestemont,Patrick

    PY - 2017/9/7

    Y1 - 2017/9/7

    N2 - In the Mekong Delta, striped catfish are faced with chronic salinity stress related to saltwater intrusion induced by global climatic changes. In this study, striped catfish juveniles were submitted to a prolonged salinity stress (up to 10 ppt) over three weeks followed by infection with a virulent bacterial strain, Edwardsiella ictaluri. Osmoregulatory parameters were investigated. In addition, a label free quantitative proteomics workflow was performed on kidneys. The workflow consisted of an initial global profiling of relative peptide abundances (by LC/MS, peak area quantification based on extracted ion currents), followed by identification (by MS/MS). The aim of the study was to highlight specific functional pathways modified during realistic salinity stress, particularly those involved in immunity. In kidney proteome, 2483 proteins were identified, of which 400 proteins were differentially expressed between the freshwater and the saline water conditions. Several pathways and functional categories were highlighted, mostly related to energy metabolism, protein metabolism, actin cytoskeleton, signaling, immunity, and detoxification. In particular, the responsiveness of proteins involved in small GTPases and Mitogen Activated Protein Kinase p38 signaling, phagolysosome maturation, and T-cells regulation is discussed. Statement of significance In the Mekong River Delta (Vietnam), striped catfish production is threatened by extensive sea water intrusion exacerbated by sea level rise. In fish, the effect of chronic exposure to salinity stress on immune capacities and response to disease has been poorly investigated. This study aims to highlight the main molecular changes occurring in the kidney during acclimation to salinity stress, particularly those involved in the immune defences of fish.

    AB - In the Mekong Delta, striped catfish are faced with chronic salinity stress related to saltwater intrusion induced by global climatic changes. In this study, striped catfish juveniles were submitted to a prolonged salinity stress (up to 10 ppt) over three weeks followed by infection with a virulent bacterial strain, Edwardsiella ictaluri. Osmoregulatory parameters were investigated. In addition, a label free quantitative proteomics workflow was performed on kidneys. The workflow consisted of an initial global profiling of relative peptide abundances (by LC/MS, peak area quantification based on extracted ion currents), followed by identification (by MS/MS). The aim of the study was to highlight specific functional pathways modified during realistic salinity stress, particularly those involved in immunity. In kidney proteome, 2483 proteins were identified, of which 400 proteins were differentially expressed between the freshwater and the saline water conditions. Several pathways and functional categories were highlighted, mostly related to energy metabolism, protein metabolism, actin cytoskeleton, signaling, immunity, and detoxification. In particular, the responsiveness of proteins involved in small GTPases and Mitogen Activated Protein Kinase p38 signaling, phagolysosome maturation, and T-cells regulation is discussed. Statement of significance In the Mekong River Delta (Vietnam), striped catfish production is threatened by extensive sea water intrusion exacerbated by sea level rise. In fish, the effect of chronic exposure to salinity stress on immune capacities and response to disease has been poorly investigated. This study aims to highlight the main molecular changes occurring in the kidney during acclimation to salinity stress, particularly those involved in the immune defences of fish.

    KW - Catfish

    KW - Immunity

    KW - Infectious disease

    KW - Label-free proteomics

    KW - Osmoregulation

    UR - http://www.scopus.com/inward/record.url?scp=85027857360&partnerID=8YFLogxK

    U2 - 10.1016/j.jprot.2017.08.005

    DO - 10.1016/j.jprot.2017.08.005

    M3 - Article

    VL - 167

    SP - 12

    EP - 24

    JO - Journal of Proteomics

    T2 - Journal of Proteomics

    JF - Journal of Proteomics

    SN - 1874-3919

    ER -