RpoE1, an extracytoplasmic function sigma factor, is a repressor of the flagellar system in Brucella melitensis

Jonathan Ferooz, Julien Lemaire, Marie Delory, Xavier De Bolle, Jean-Jacques Letesson

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

The genome of Brucella melitensis contains genes coding for the sigma factors RpoD, RpoN, RpoH1, RpoH2, RpoE1 and RpoE2. Previously published data show that B. melitensis is flagellated and that an rpoE1 mutant overexpresses the flagellar protein FlgE. In this study, we demonstrate that mutation of rpoE1 causes an overexpression of the flagellar genes fliF, flgE, fliC, flaF and flbT, correlating with the production of a longer filament and thereby demonstrating that RpoE1 acts as a flagellar repressor. Moreover, mutation of rpoE1 increases the promoter activity of the flagellar master regulator ftcR, suggesting that RpoE1 acts upstream of ftcR. Together, these data show that RpoE1 represses the flagellar synthesis and filament length in B. melitensis.
langue originaleAnglais
Pages (de - à)1263-8
Nombre de pages6
journalMicrobiology
Volume157
Numéro de publication5
Les DOIs
étatPublié - 1 mai 2011

Empreinte digitale

Brucella melitensis
Sigma Factor
Mutation
Genes
Genome
Proteins

Citer ceci

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abstract = "The genome of Brucella melitensis contains genes coding for the sigma factors RpoD, RpoN, RpoH1, RpoH2, RpoE1 and RpoE2. Previously published data show that B. melitensis is flagellated and that an rpoE1 mutant overexpresses the flagellar protein FlgE. In this study, we demonstrate that mutation of rpoE1 causes an overexpression of the flagellar genes fliF, flgE, fliC, flaF and flbT, correlating with the production of a longer filament and thereby demonstrating that RpoE1 acts as a flagellar repressor. Moreover, mutation of rpoE1 increases the promoter activity of the flagellar master regulator ftcR, suggesting that RpoE1 acts upstream of ftcR. Together, these data show that RpoE1 represses the flagellar synthesis and filament length in B. melitensis.",
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RpoE1, an extracytoplasmic function sigma factor, is a repressor of the flagellar system in Brucella melitensis. / Ferooz, Jonathan; Lemaire, Julien; Delory, Marie; De Bolle, Xavier; Letesson, Jean-Jacques.

Dans: Microbiology, Vol 157, Numéro 5, 01.05.2011, p. 1263-8.

Résultats de recherche: Contribution à un journal/une revueArticle

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AB - The genome of Brucella melitensis contains genes coding for the sigma factors RpoD, RpoN, RpoH1, RpoH2, RpoE1 and RpoE2. Previously published data show that B. melitensis is flagellated and that an rpoE1 mutant overexpresses the flagellar protein FlgE. In this study, we demonstrate that mutation of rpoE1 causes an overexpression of the flagellar genes fliF, flgE, fliC, flaF and flbT, correlating with the production of a longer filament and thereby demonstrating that RpoE1 acts as a flagellar repressor. Moreover, mutation of rpoE1 increases the promoter activity of the flagellar master regulator ftcR, suggesting that RpoE1 acts upstream of ftcR. Together, these data show that RpoE1 represses the flagellar synthesis and filament length in B. melitensis.

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