TY - JOUR
T1 - Role of nuclear factor-κB in colon cancer cell apoptosis mediated by aminopyropheophorbide photosensitization
AU - Matroule, Jean-Yves
AU - Hellin, Anne Cécile
AU - Morlière, Patrice
AU - Fabiano, A. S.
AU - Santus, René
AU - Merville, Marie Paule
AU - Piette, Jacques
AU - Piette, Jacques
PY - 1999/10/1
Y1 - 1999/10/1
N2 - Aminopyropheophorbide (APP) is a second generation of photosensitizer for photodynamic therapy (PDT). We demonstrated that APP strongly absorbed red light and, after being taken up by colon cancer cells (HCT-116 cells), was localized in cytoplasmic and internal membranes but not in mitochondria. The APP-mediated photosensitization was cytotoxic for HCT-116 cells through an induction of apoptosis. Indeed, DNA fragmentation (DNA laddering and terminal deoxyuridine nick-end labeling) and chromatin condensation (4′,6-diamidine-2′-phenylindole staining) could be visualized soon after photosensitization. Because nuclear factor (NF)-κB is involved in the response to many photosensitizers, we also demonstrated its nuclear translocation in two waves: a rapid and transient one, followed by a slow and sustained phase. The NF-κB turned out to be involved in an antiapoptotic response to APP-mediated photosensitization because the HCT-116 cell line expressing the dominant negative mutant of inhibitor-κBα was more sensitive to apoptosis as measured by DNA fragmentation and caspase activation. These data unambiguously show that a membrane-located photosensitizer can lead to effective apoptosis, reinforcing the idea that PDT can be an effective means to eradicate colon cancer cells.
AB - Aminopyropheophorbide (APP) is a second generation of photosensitizer for photodynamic therapy (PDT). We demonstrated that APP strongly absorbed red light and, after being taken up by colon cancer cells (HCT-116 cells), was localized in cytoplasmic and internal membranes but not in mitochondria. The APP-mediated photosensitization was cytotoxic for HCT-116 cells through an induction of apoptosis. Indeed, DNA fragmentation (DNA laddering and terminal deoxyuridine nick-end labeling) and chromatin condensation (4′,6-diamidine-2′-phenylindole staining) could be visualized soon after photosensitization. Because nuclear factor (NF)-κB is involved in the response to many photosensitizers, we also demonstrated its nuclear translocation in two waves: a rapid and transient one, followed by a slow and sustained phase. The NF-κB turned out to be involved in an antiapoptotic response to APP-mediated photosensitization because the HCT-116 cell line expressing the dominant negative mutant of inhibitor-κBα was more sensitive to apoptosis as measured by DNA fragmentation and caspase activation. These data unambiguously show that a membrane-located photosensitizer can lead to effective apoptosis, reinforcing the idea that PDT can be an effective means to eradicate colon cancer cells.
UR - http://www.scopus.com/inward/record.url?scp=0033212122&partnerID=8YFLogxK
M3 - Article
C2 - 10546550
AN - SCOPUS:0033212122
SN - 0031-8655
VL - 70
SP - 540
EP - 548
JO - Photochemistry and Photobiology
JF - Photochemistry and Photobiology
IS - 4
ER -