Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice

Jean-Michel Vandeweerd, Fanny Hontoir, Alexis De Knoop, Kathleen De Swert, Charles Nicaise

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

Phrenic motor neurons are cervical motor neurons originating from C3 to C6 levels in most mammalian species. Axonal projections converge into phrenic nerves innervating the respiratory diaphragm. In spinal cord slices, phrenic motor neurons cannot be identified from other motor neurons on morphological or biochemical criteria. We provide the description of procedures for visualizing phrenic motor neuron cell bodies in mice, following intrapleural injections of cholera toxin subunit beta (CTB) conjugated to a fluorophore. This fluorescent neuroanatomical tracer has the ability to be caught up at the diaphragm neuromuscular junction, be carried retrogradely along the phrenic axons and reach the phrenic cell bodies. Two methodological approaches of intrapleural CTB delivery are compared: transdiaphragmatic versus transthoracic injections. Both approaches are successful and result in similar number of CTB-labeled phrenic motor neurons. In conclusion, these techniques can be applied to visualize or quantify the phrenic motor neurons in various experimental studies such as those focused on the diaphragm-phrenic circuitry.

langue originaleAnglais
journalJournal of visualized experiments : JoVE
Numéro de publication132
Les DOIs
étatPublié - 22 févr. 2018

Empreinte digitale

Motor Neurons
Diaphragm
Neurons
Cholera Toxin
Diaphragms
Cells
Fluorophores
Phrenic Nerve
Injections
Neuromuscular Junction
Axons
Spinal Cord

Citer ceci

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title = "Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice",
abstract = "Phrenic motor neurons are cervical motor neurons originating from C3 to C6 levels in most mammalian species. Axonal projections converge into phrenic nerves innervating the respiratory diaphragm. In spinal cord slices, phrenic motor neurons cannot be identified from other motor neurons on morphological or biochemical criteria. We provide the description of procedures for visualizing phrenic motor neuron cell bodies in mice, following intrapleural injections of cholera toxin subunit beta (CTB) conjugated to a fluorophore. This fluorescent neuroanatomical tracer has the ability to be caught up at the diaphragm neuromuscular junction, be carried retrogradely along the phrenic axons and reach the phrenic cell bodies. Two methodological approaches of intrapleural CTB delivery are compared: transdiaphragmatic versus transthoracic injections. Both approaches are successful and result in similar number of CTB-labeled phrenic motor neurons. In conclusion, these techniques can be applied to visualize or quantify the phrenic motor neurons in various experimental studies such as those focused on the diaphragm-phrenic circuitry.",
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Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice. / Vandeweerd, Jean-Michel; Hontoir, Fanny; De Knoop, Alexis; De Swert, Kathleen; Nicaise, Charles.

Dans: Journal of visualized experiments : JoVE, Numéro 132, 22.02.2018.

Résultats de recherche: Contribution à un journal/une revueArticle

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