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Résumé
Results. Our results indicate that both B. abortus S2308 and B. melitensis 16M strains are able to invade and replicate in Atg5-deficient fibroblasts, suggesting that the canonical Atg5-dependent macroautophagic pathway is dispensable for Brucella replication. The number of viable bacteria was even slightly higher in Atg5-/- fibroblasts than in wild-type fibroblasts. This increase could be due to a more efficient uptake or to a better survival rate of bacteria before the beginning of the replication in Atg5-deficient cells as compared to wild-type cells. Moreover, our data show that the infection with B. abortus or with B. melitensis does not stimulate neither the conversion of LC3-I into LC3-II nor the membrane recruitment of LC3 onto the BCV.
Conclusion. Our study suggests that like Brucella abortus, Brucella melitensis does not subvert the canonical macroautophagy to reach its replicative niche or to stimulate its replication.
langue originale | Anglais |
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Numéro d'article | 14 |
journal | BMC microbiology |
Volume | 14 |
Numéro de publication | 1 |
Les DOIs | |
Etat de la publication | Publié - 2 sept. 2014 |
Empreinte digitale
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