Reconstruction of normal and pathological human epidermis on polycarbonate filter

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Résumé

This chapter provides methods suitable for the culture of primary human keratinocytes in serum-free culture conditions, starting from very small skin biopsies. It also explains procedures required for reconstruction of a stratified epidermis on polycarbonate filter, starting from keratinocytes cultured in serum-free conditions. Tissues reconstructed according to this method have been proven suitable for characterization of epidermal morphogenesis and for in vitro studies of the epidermal barrier. Utilization of the same method for successful isolation of keratinocytes from a patient suffering from Darier's disease and the reconstruction of a pathological epidermis which displays the same histological features as in vivo are also presented.

langue originaleAnglais
titreEpidermal cells 3rd Edition
Sous-titreMethods in Molecular Biology
rédacteurs en chefKursad Turksen
Lieu de publicationClifton, N.J.
EditeurHumana Press
Pages191-201
Nombre de pages11
Volume1195
ISBN (imprimé)9781493912230
Les DOIs
étatPublié - 2014

Série de publications

NomMethods in Molecular Biology
Volume1195
ISSN (imprimé)10643745

    Empreinte digitale

Contient cette citation

De Vuyst, E., Charlier, C., Giltaire, S., De Glas, V., De Rouvroit, C. L., & Poumay, Y. (2014). Reconstruction of normal and pathological human epidermis on polycarbonate filter. Dans K. Turksen (Ed.), Epidermal cells 3rd Edition: Methods in Molecular Biology (Vol 1195, p. 191-201). (Methods in Molecular Biology; Vol 1195). Clifton, N.J.: Humana Press. https://doi.org/10.1007/7651-2013-40