Reconstruction of normal and pathological human epidermis on polycarbonate filter

Evelyne De Vuyst; Céline Charlier; Séverine Giltaire; Valérie De Glas; Catherine Lambert De Rouvroit; Yves Poumay

doi:10.1007/7651-2013-40

Reconstruction of normal and pathological human epidermis on polycarbonate filter

Evelyne De Vuyst, Céline Charlier, Séverine Giltaire, Valérie De Glas, Catherine Lambert De Rouvroit, Yves Poumay

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Résumé

This chapter provides methods suitable for the culture of primary human keratinocytes in serum-free culture conditions, starting from very small skin biopsies. It also explains procedures required for reconstruction of a stratified epidermis on polycarbonate filter, starting from keratinocytes cultured in serum-free conditions. Tissues reconstructed according to this method have been proven suitable for characterization of epidermal morphogenesis and for in vitro studies of the epidermal barrier. Utilization of the same method for successful isolation of keratinocytes from a patient suffering from Darier's disease and the reconstruction of a pathological epidermis which displays the same histological features as in vivo are also presented.

langue originale	Anglais
titre	Epidermal cells 3rd Edition
Sous-titre	Methods in Molecular Biology
rédacteurs en chef	Kursad Turksen
Lieu de publication	Clifton, N.J.
Editeur	Humana Press
Pages	191-201
Nombre de pages	11
Volume	1195
ISBN (imprimé)	9781493912230
Les DOIs	https://doi.org/10.1007/7651-2013-40
Etat de la publication	Publié - 2014

Série de publications

Nom	Methods in Molecular Biology
Volume	1195
ISSN (imprimé)	10643745

SDG des Nations Unies

Ce résultat contribue à ou aux Objectifs de développement durable suivants

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10.1007/7651-2013-40

Epidermal Cell-Chapter De Vuyst et almanuscrit soumis, 457 KB

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Etude de la perturbation de la barrière d'épidermes reconstruits in vitro dans le but de mimer les caractéristiques épidermiques de la dermatite atopique.
DE VUYST, E. (Chercheur) & Poumay, Y. (Co-investigateur)
14/03/12 → 20/12/16
Projet: Projet de thèse

Plateforme d'histologie
Nicaise, C. (!!Manager), Poumay, Y. (!!Manager) & Bielarz, V. (!!Other)
Plateforme technologique Morphologie, imagerie
Equipement/installations: Equipement

Barrier alterations in a reconstructed epidermis as a model for atopic dermatitis
De Vuyst, E. (Auteur)Poumay, Y. (Promoteur), Lambert De Rouvroit, C. (Copromoteur), Michiels, C. (Président), Gillet, J.-P. (Jury), Brandner, J. (Jury), Salmon, M. (Jury) & Nikkels, A. F. (Jury), 20 déc. 2016
Student thesis: Doc types › Docteur en Sciences Biomédicales et Pharmaceutiques

Contient cette citation

De Vuyst, E., Charlier, C., Giltaire, S., De Glas, V., De Rouvroit, C. L., & Poumay, Y. (2014). Reconstruction of normal and pathological human epidermis on polycarbonate filter. Dans K. Turksen (Ed.), Epidermal cells 3rd Edition: Methods in Molecular Biology (Vol 1195, p. 191-201). (Methods in Molecular Biology; Vol 1195). Humana Press. https://doi.org/10.1007/7651-2013-40

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abstract = "This chapter provides methods suitable for the culture of primary human keratinocytes in serum-free culture conditions, starting from very small skin biopsies. It also explains procedures required for reconstruction of a stratified epidermis on polycarbonate filter, starting from keratinocytes cultured in serum-free conditions. Tissues reconstructed according to this method have been proven suitable for characterization of epidermal morphogenesis and for in vitro studies of the epidermal barrier. Utilization of the same method for successful isolation of keratinocytes from a patient suffering from Darier's disease and the reconstruction of a pathological epidermis which displays the same histological features as in vivo are also presented.",

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De Vuyst, E, Charlier, C, Giltaire, S, De Glas, V, De Rouvroit, CL & Poumay, Y 2014, Reconstruction of normal and pathological human epidermis on polycarbonate filter. Dans K Turksen (Ed.), Epidermal cells 3rd Edition: Methods in Molecular Biology. VOL. 1195, Methods in Molecular Biology, VOL. 1195, Humana Press, Clifton, N.J., p. 191-201. https://doi.org/10.1007/7651-2013-40

Reconstruction of normal and pathological human epidermis on polycarbonate filter. / De Vuyst, Evelyne; Charlier, Céline; Giltaire, Séverine et al.
Epidermal cells 3rd Edition: Methods in Molecular Biology. Ed. / Kursad Turksen. Vol 1195 Clifton, N.J.: Humana Press, 2014. p. 191-201 (Methods in Molecular Biology; Vol 1195).

Résultats de recherche: Contribution dans un livre/un catalogue/un rapport/dans les actes d'une conférence › Chapitre (revu par des pairs) › Revue par des pairs

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AU - Poumay, Yves

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AB - This chapter provides methods suitable for the culture of primary human keratinocytes in serum-free culture conditions, starting from very small skin biopsies. It also explains procedures required for reconstruction of a stratified epidermis on polycarbonate filter, starting from keratinocytes cultured in serum-free conditions. Tissues reconstructed according to this method have been proven suitable for characterization of epidermal morphogenesis and for in vitro studies of the epidermal barrier. Utilization of the same method for successful isolation of keratinocytes from a patient suffering from Darier's disease and the reconstruction of a pathological epidermis which displays the same histological features as in vivo are also presented.

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Reconstruction of normal and pathological human epidermis on polycarbonate filter

Résumé

Série de publications

SDG des Nations Unies

Accès au document

Autres fichiers et liens

Empreinte digitale

Projets

Etude de la perturbation de la barrière d'épidermes reconstruits in vitro dans le but de mimer les caractéristiques épidermiques de la dermatite atopique.

Équipement

Plateforme d'histologie

Thèses de l'étudiant

Barrier alterations in a reconstructed epidermis as a model for atopic dermatitis

Contient cette citation