TY - JOUR
T1 - Quality control data of physiological and immunological biomarkers measured in serum and plasma
AU - Jansen, Eugène
AU - Beekhof, Piet
AU - Cremers, Johannes
AU - Weinberger, Birgit
AU - Fiegl, Simone
AU - Toussaint, Olivier
AU - Bernhard, Jürgen
AU - Gonos, Efstathios
AU - Capri, Miriam
AU - Franceschi, Claudio
AU - Sikora, Ewa
AU - Moreno-Villanueva, María
AU - Breusing, Nicolle
AU - Grune, Tilman
AU - Bürkle, Alexander
AU - Dollé, Martijn E T
N1 - Copyright © 2015. Published by Elsevier Ireland Ltd.
PY - 2015/1/30
Y1 - 2015/1/30
N2 - In two work packages of the MARK-AGE project, 37 immunological and physiological biomarkers were measured in 3637 serum, plasma or blood samples in five batches during a period of 4 years.The quality of the serum and plasma samples was very good as judged by the low number of biomarker measurements (only 0.2%) that were rejected because of a high hemolysis, icteria or lipemia of the samples.Using quality control samples, day-to-day and batch variations were determined. The mean inter-assay variation of the five batches were all below 8%, with an average inter-assay coefficient of variation of all biomarkers of 4.0%. Also the precision of the measurements was very good, because all measurements were between 90% and 115% of the defined target values.A possible mix-up of samples was determined by comparison of the extreme testosterone levels of men and women. It was concluded that 3% of the sample identification could be mixed-up.Considering the complex procedure from collection to analysis, including preparation, handling, shipment and storage, of the samples in the MARK-AGE project, both the quality of the samples and the quality of the measurements are very good.
AB - In two work packages of the MARK-AGE project, 37 immunological and physiological biomarkers were measured in 3637 serum, plasma or blood samples in five batches during a period of 4 years.The quality of the serum and plasma samples was very good as judged by the low number of biomarker measurements (only 0.2%) that were rejected because of a high hemolysis, icteria or lipemia of the samples.Using quality control samples, day-to-day and batch variations were determined. The mean inter-assay variation of the five batches were all below 8%, with an average inter-assay coefficient of variation of all biomarkers of 4.0%. Also the precision of the measurements was very good, because all measurements were between 90% and 115% of the defined target values.A possible mix-up of samples was determined by comparison of the extreme testosterone levels of men and women. It was concluded that 3% of the sample identification could be mixed-up.Considering the complex procedure from collection to analysis, including preparation, handling, shipment and storage, of the samples in the MARK-AGE project, both the quality of the samples and the quality of the measurements are very good.
KW - Biomarkers
KW - Hemolysis
KW - Quality control
UR - http://www.scopus.com/inward/record.url?scp=84947040058&partnerID=8YFLogxK
U2 - 10.1016/j.mad.2015.06.004
DO - 10.1016/j.mad.2015.06.004
M3 - Article
C2 - 26166476
SN - 0047-6374
VL - 151
SP - 54
EP - 59
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
ER -
