Proteomics as a way to identify extra-radicular fungal proteins from Glomus intraradices - RiT-DNA carrot root mycorrhizas

E. Dumas-Gaudot, B. Valot, G. Bestel-Corre, G. Recorbet, M. St-Arnaud, B. Fontaine, M. Dieu, Martine Raes, R.S. Saravanan, S. Gianinazzi

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Résumé

To identify fungal proteins involved in the arbuscular mycorrhizal symbiosis, root-inducing transferred-DNA transformed roots of carrot (Daucus carota L.) were in vitro inoculated with Glomus intraradices. Proteins extracted from the extra-radical fungus were analysed by two-dimensional gel electrophoresis. A fungal reference map displaying 438 spots was set up. Four proteins, among the 14 selected for tandem mass spectrometry analysis, were identified including a NmrA-like protein, an oxido-reductase, a heat-shock protein and an ATP synthase β mitochondrial precursor. The possible fungal origin of a MYK15-like protein found in mycorrhizal roots was further discussed. This is the first report of arbuscular mycorrhizal fungal protein identifications by using a proteomic approach.
langue originaleAnglais
Pages (de - à)401-411
Nombre de pages11
journalFEMS Microbiology Ecology
Volume48
Numéro de publication3
Les DOIs
Etat de la publicationPublié - 1 juin 2004

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