TY - JOUR
T1 - Proteasome and antioxidant responses in Cottus gobio during a combined exposure to heat stress and cadmium
AU - Dorts, Jennifer
AU - Bauwin, Aurélie
AU - Kestemont, Patrick
AU - Jolly, Sabrina
AU - Sanchez, Wilfried
AU - Silvestre, Frédéric
PY - 2012/3/1
Y1 - 2012/3/1
N2 - Temperature and trace metals are common environmental stressors, and their importance is increasing due to global climate change and anthropogenic pollution. Oxidative damage and antioxidant properties have been studied in liver and gills of the European bullhead (Cottus gobio) subjected to cadmium (CdCl 2 at nominal concentrations of 0.01 and 1 mg/L) for 4 days at either 15 °C or 21°C. First, exposure to 1 mg Cd/L induced a high mortality rate (67%) in fish held at 21°C. Regarding the antioxidant enzymes, exposure to 0.01 mg Cd/L significantly increased the activity of superoxide dismutase (SOD) and decreased the activity of glutathione reductase (GR) in liver, independently of heat stress. In gills, exposure to 21°C resulted in a significantly increased activity of glutathione peroxidase (GPx), whereas the activity of glutathione S-transferase (GST) was significantly reduced as compared to fish exposed to 15°C. Furthermore, regardless of Cd stress, exposure to elevated temperature resulted in a significant decrease of lipid peroxidation (LPO) level in liver and in a significant increase in the activity of chymotrypsin-like 20S proteasome in both studied tissues of C. gobio. Overall, the present results indicated that elevated temperature and cadmium exposure independently influenced the antioxidant defense system in bullhead with clear tissue-specific and stress-specific antioxidant responses. Further, elevated temperature affected the hepatic lipid peroxidation and the activity of 20S proteasome in both tissues.
AB - Temperature and trace metals are common environmental stressors, and their importance is increasing due to global climate change and anthropogenic pollution. Oxidative damage and antioxidant properties have been studied in liver and gills of the European bullhead (Cottus gobio) subjected to cadmium (CdCl 2 at nominal concentrations of 0.01 and 1 mg/L) for 4 days at either 15 °C or 21°C. First, exposure to 1 mg Cd/L induced a high mortality rate (67%) in fish held at 21°C. Regarding the antioxidant enzymes, exposure to 0.01 mg Cd/L significantly increased the activity of superoxide dismutase (SOD) and decreased the activity of glutathione reductase (GR) in liver, independently of heat stress. In gills, exposure to 21°C resulted in a significantly increased activity of glutathione peroxidase (GPx), whereas the activity of glutathione S-transferase (GST) was significantly reduced as compared to fish exposed to 15°C. Furthermore, regardless of Cd stress, exposure to elevated temperature resulted in a significant decrease of lipid peroxidation (LPO) level in liver and in a significant increase in the activity of chymotrypsin-like 20S proteasome in both studied tissues of C. gobio. Overall, the present results indicated that elevated temperature and cadmium exposure independently influenced the antioxidant defense system in bullhead with clear tissue-specific and stress-specific antioxidant responses. Further, elevated temperature affected the hepatic lipid peroxidation and the activity of 20S proteasome in both tissues.
KW - Antioxidant enzymes
KW - Cadmium
KW - Fish
KW - Heat stress
KW - Oxidative stress
KW - Proteasomal activity
KW - Temperature
UR - http://www.scopus.com/inward/record.url?scp=84655164290&partnerID=8YFLogxK
U2 - 10.1016/j.cbpc.2011.10.003
DO - 10.1016/j.cbpc.2011.10.003
M3 - Article
C2 - 22033351
AN - SCOPUS:84655164290
SN - 1532-0456
VL - 155
SP - 318
EP - 324
JO - Comparative Biochemistry and Physiology. Part C: Toxicology & Pharmacology
JF - Comparative Biochemistry and Physiology. Part C: Toxicology & Pharmacology
IS - 2
ER -