Résumé
Infrared/visible sum-frequency generation (SFG) spectroscopy is
used to study the recognition of a protein (avidin) by a derived
vitamin (biocytin) adsorbed on a calcium fluoride substrate. The
specificity of the process is tested by replacing avidin with bovine
serum albumin or presaturated avidin. The SFG spectroscopy
shows drastic modifications in the CH and NH spectral ranges
only upon exposure of the biocytin film to avidin. The comparison
of the SFG data with Fourier transform infrared reflection absorption
spectra (FT-IRRAS) in the same spectral ranges illustrates
the advantages of nonlinear spectroscopy for studying and detecting
recognition between biomolecules.
langue originale | Anglais |
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Pages (de - à) | 1719-1725 |
Nombre de pages | 7 |
journal | ChemPhysChem |
Volume | 5 |
Numéro de publication | 11 |
Etat de la publication | Publié - 2004 |