Positive selection of recombinant plasmids based on the EcoK restriction activity of Escherichia coli K-12

O De Backer, P Chomez, E De Plaen

Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs

Résumé

We have constructed a pTZ19R-derived vector which allows efficient positive selection of recombinant plasmids. The system uses the EcoK restriction activity of Escherichia coli K-12 to select against non-recombinant plasmids. The vector contains an EcoK site which, if deleted or disrupted by ligating a DNA fragment, yields recombinant plasmids that are no longer susceptible to EcoK restriction when transformed into a restriction-proficient E. coli host.
langue originaleAnglais
Pages (de - à)197-8
Nombre de pages2
journalGene
Volume150
Numéro de publication1
Etat de la publicationPublié - 1994

Empreinte digitale

Examiner les sujets de recherche de « Positive selection of recombinant plasmids based on the EcoK restriction activity of Escherichia coli K-12 ». Ensemble, ils forment une empreinte digitale unique.

Contient cette citation