Résumé
We have constructed a pTZ19R-derived vector which allows efficient positive selection of recombinant plasmids. The system uses the EcoK restriction activity of Escherichia coli K-12 to select against non-recombinant plasmids. The vector contains an EcoK site which, if deleted or disrupted by ligating a DNA fragment, yields recombinant plasmids that are no longer susceptible to EcoK restriction when transformed into a restriction-proficient E. coli host.
langue originale | Anglais |
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Pages (de - à) | 197-8 |
Nombre de pages | 2 |
journal | Gene |
Volume | 150 |
Numéro de publication | 1 |
Etat de la publication | Publié - 1994 |