Platelet lipidome alterations in septic shock: a matched case-control study

Emma de Cartier d'Yves; Melanie Dechamps; Jérôme Ambroise; Anik Forest; Caroline Daneault; Alessandro Campion; Valentine Robaux; Julien De Poortere; Marie Octave; Audrey Ginion; Laurence Pirotton; Gabriele Muscia; Claudia Tersteeg; Damien Gruson; Marie-Astrid Van Dievoet; Jonathan Douxfils; Hélène Haguet; Laure Morimont; Marc Derive; Virginie Montiel; Luc Bertrand; Christine Des Rosiers; Sandrine Horman; Christophe Beauloye

doi:10.1016/j.rpth.2025.103332

Platelet lipidome alterations in septic shock: a matched case-control study

Emma de Cartier d'Yves
, Melanie Dechamps
, Jérôme Ambroise
, Anik Forest
, Caroline Daneault
, Alessandro Campion
, Valentine Robaux
, Julien De Poortere
, Marie Octave
, Audrey Ginion
, Laurence Pirotton
, Gabriele Muscia
, Claudia Tersteeg
, Damien Gruson
, Marie-Astrid Van Dievoet
, Jonathan Douxfils
, Hélène Haguet
, Laure Morimont
, Marc Derive
, Virginie Montiel

Luc Bertrand, Christine Des Rosiers, Sandrine Horman, Christophe Beauloye

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

Résumé

BACKGROUND: Platelets play a central role in hemostatic and inflammatory responses during septic shock, with lipids being essential for their function. However, the specific lipidomic alterations occurring in platelets during septic shock remain poorly understood.

OBJECTIVES: This study aimed to characterize platelet lipidomic changes in septic shock and investigate their associations with disease severity.

METHODS: In this matched case-control study, platelets were isolated from 49 septic shock patients and 47 nonseptic controls (matched for age, gender, and comorbidities). Lipidomic profiling was performed using untargeted lipidomics to identify significant alterations in the platelet lipidome. Associations among lipid changes, clinical data, and plasma biomarkers of coagulopathy and inflammation were explored.

RESULTS: More than 60% of the annotated platelet lipids were significantly altered in septic shock. Cholesteryl esters, sphingomyelins, lysophosphatidylcholines, and ether-lipids were significantly reduced, while ceramide levels increased. Fatty acyl chain remodeling displayed distinct patterns, with polyunsaturated fatty acids increasing in triacylglycerols and decreasing in phospholipids. Lipid alterations were strongly associated with thrombocytopenia, and lysophosphatidylcholine levels inversely correlated with disease severity, as indicated by the Sequential Organ Failure Assessment score.

CONCLUSIONS: Septic shock induces significant disruptions in the platelet lipidome, with the extent of these alterations correlating with sepsis-associated thrombocytopenia severity. The observed changes affect multiple lipid classes, surpassing those reported under physiological conditions or in other diseases. These findings highlight the impact of sepsis-driven dysregulated inflammation and coagulopathy on platelet lipid composition, providing new insights into sepsis pathophysiology.

langue originale	Anglais
Numéro d'article	103332
Pages (de - à)	103332
journal	Research and Practice in Thrombosis and Haemostasis
Volume	10
Numéro de publication	1
Les DOIs	https://doi.org/10.1016/j.rpth.2025.103332
Etat de la publication	Publié - janv. 2026

Financement

This work was supported by grants from the “Fondation Saint- Luc” (Brussels, Belgium) and the “Fonds National de la Recherche Scientifique et Médicale” (FNRS, Belgium). The Division of Cardiology at “Cliniques Universitaires Saint-Luc” (Belgium) has received unrestricted research grants from AstraZeneca (Belgium). E.d.C.d. is supported by a PhD fellowship from the FNRS. M. Dechamps is Clinical Master Specialist and a PhD candidate funded by the FNRS. S.H. is senior research associate at FNRS. QUALIblood s.a. provided the cytokine profile analyses.

Bailleurs de fonds
Fonds National de la Recherche Scientifique et Médicale
Cliniques Universitaires Saint-Luc
Fondation Saint
Fonds De La Recherche Scientifique - FNRS
AstraZeneca NV

SDG des Nations Unies

Ce résultat contribue à ou aux Objectifs de développement durable suivants

SDG 5 Égalité des sexes

Accès au document

10.1016/j.rpth.2025.103332

Publi_platelet_lipidome_alterationsVersion finale publiée, 3,34 MBLicense: CC BY-NC-ND

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Catholic University Louvain (UCLouvain) Researchers Report on Findings in Sepsis (Platelet lipidome alterations in septic shock: a matched case-control study)
Douxfils, J., Haguet, H. & Beauloye, C.
3/02/26
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Contient cette citation

de Cartier d'Yves, E., Dechamps, M., Ambroise, J., Forest, A., Daneault, C., Campion, A., Robaux, V., De Poortere, J., Octave, M., Ginion, A., Pirotton, L., Muscia, G., Tersteeg, C., Gruson, D., Van Dievoet, M.-A., Douxfils, J., Haguet, H., Morimont, L., Derive, M., ... Beauloye, C. (2026). Platelet lipidome alterations in septic shock: a matched case-control study. Research and Practice in Thrombosis and Haemostasis, 10(1), 103332. Article 103332. https://doi.org/10.1016/j.rpth.2025.103332

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title = "Platelet lipidome alterations in septic shock: a matched case-control study",

abstract = "BACKGROUND: Platelets play a central role in hemostatic and inflammatory responses during septic shock, with lipids being essential for their function. However, the specific lipidomic alterations occurring in platelets during septic shock remain poorly understood.OBJECTIVES: This study aimed to characterize platelet lipidomic changes in septic shock and investigate their associations with disease severity.METHODS: In this matched case-control study, platelets were isolated from 49 septic shock patients and 47 nonseptic controls (matched for age, gender, and comorbidities). Lipidomic profiling was performed using untargeted lipidomics to identify significant alterations in the platelet lipidome. Associations among lipid changes, clinical data, and plasma biomarkers of coagulopathy and inflammation were explored.RESULTS: More than 60\% of the annotated platelet lipids were significantly altered in septic shock. Cholesteryl esters, sphingomyelins, lysophosphatidylcholines, and ether-lipids were significantly reduced, while ceramide levels increased. Fatty acyl chain remodeling displayed distinct patterns, with polyunsaturated fatty acids increasing in triacylglycerols and decreasing in phospholipids. Lipid alterations were strongly associated with thrombocytopenia, and lysophosphatidylcholine levels inversely correlated with disease severity, as indicated by the Sequential Organ Failure Assessment score.CONCLUSIONS: Septic shock induces significant disruptions in the platelet lipidome, with the extent of these alterations correlating with sepsis-associated thrombocytopenia severity. The observed changes affect multiple lipid classes, surpassing those reported under physiological conditions or in other diseases. These findings highlight the impact of sepsis-driven dysregulated inflammation and coagulopathy on platelet lipid composition, providing new insights into sepsis pathophysiology.",

keywords = "blood platelet, coagulopathy, inflammation, lipidomics, septic shock",

author = "\{de Cartier d'Yves\}, Emma and Melanie Dechamps and J{\'e}r{\^o}me Ambroise and Anik Forest and Caroline Daneault and Alessandro Campion and Valentine Robaux and \{De Poortere\}, Julien and Marie Octave and Audrey Ginion and Laurence Pirotton and Gabriele Muscia and Claudia Tersteeg and Damien Gruson and \{Van Dievoet\}, Marie-Astrid and Jonathan Douxfils and H{\'e}l{\`e}ne Haguet and Laure Morimont and Marc Derive and Virginie Montiel and Luc Bertrand and Rosiers, \{Christine Des\} and Sandrine Horman and Christophe Beauloye",

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year = "2026",

month = jan,

doi = "10.1016/j.rpth.2025.103332",

language = "English",

volume = "10",

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de Cartier d'Yves, E, Dechamps, M, Ambroise, J, Forest, A, Daneault, C, Campion, A, Robaux, V, De Poortere, J, Octave, M, Ginion, A, Pirotton, L, Muscia, G, Tersteeg, C, Gruson, D, Van Dievoet, M-A, Douxfils, J , Haguet, H , Morimont, L, Derive, M, Montiel, V, Bertrand, L, Rosiers, CD, Horman, S & Beauloye, C 2026, 'Platelet lipidome alterations in septic shock: a matched case-control study', Research and Practice in Thrombosis and Haemostasis, VOL. 10, Numéro 1, 103332, p. 103332. https://doi.org/10.1016/j.rpth.2025.103332

TY - JOUR

T1 - Platelet lipidome alterations in septic shock

T2 - a matched case-control study

AU - de Cartier d'Yves, Emma

AU - Dechamps, Melanie

AU - Ambroise, Jérôme

AU - Forest, Anik

AU - Daneault, Caroline

AU - Campion, Alessandro

AU - Robaux, Valentine

AU - De Poortere, Julien

AU - Octave, Marie

AU - Ginion, Audrey

AU - Pirotton, Laurence

AU - Muscia, Gabriele

AU - Tersteeg, Claudia

AU - Gruson, Damien

AU - Van Dievoet, Marie-Astrid

AU - Douxfils, Jonathan

AU - Haguet, Hélène

AU - Morimont, Laure

AU - Derive, Marc

AU - Montiel, Virginie

AU - Bertrand, Luc

AU - Rosiers, Christine Des

AU - Horman, Sandrine

AU - Beauloye, Christophe

PY - 2026/1

Y1 - 2026/1

N2 - BACKGROUND: Platelets play a central role in hemostatic and inflammatory responses during septic shock, with lipids being essential for their function. However, the specific lipidomic alterations occurring in platelets during septic shock remain poorly understood.OBJECTIVES: This study aimed to characterize platelet lipidomic changes in septic shock and investigate their associations with disease severity.METHODS: In this matched case-control study, platelets were isolated from 49 septic shock patients and 47 nonseptic controls (matched for age, gender, and comorbidities). Lipidomic profiling was performed using untargeted lipidomics to identify significant alterations in the platelet lipidome. Associations among lipid changes, clinical data, and plasma biomarkers of coagulopathy and inflammation were explored.RESULTS: More than 60% of the annotated platelet lipids were significantly altered in septic shock. Cholesteryl esters, sphingomyelins, lysophosphatidylcholines, and ether-lipids were significantly reduced, while ceramide levels increased. Fatty acyl chain remodeling displayed distinct patterns, with polyunsaturated fatty acids increasing in triacylglycerols and decreasing in phospholipids. Lipid alterations were strongly associated with thrombocytopenia, and lysophosphatidylcholine levels inversely correlated with disease severity, as indicated by the Sequential Organ Failure Assessment score.CONCLUSIONS: Septic shock induces significant disruptions in the platelet lipidome, with the extent of these alterations correlating with sepsis-associated thrombocytopenia severity. The observed changes affect multiple lipid classes, surpassing those reported under physiological conditions or in other diseases. These findings highlight the impact of sepsis-driven dysregulated inflammation and coagulopathy on platelet lipid composition, providing new insights into sepsis pathophysiology.

AB - BACKGROUND: Platelets play a central role in hemostatic and inflammatory responses during septic shock, with lipids being essential for their function. However, the specific lipidomic alterations occurring in platelets during septic shock remain poorly understood.OBJECTIVES: This study aimed to characterize platelet lipidomic changes in septic shock and investigate their associations with disease severity.METHODS: In this matched case-control study, platelets were isolated from 49 septic shock patients and 47 nonseptic controls (matched for age, gender, and comorbidities). Lipidomic profiling was performed using untargeted lipidomics to identify significant alterations in the platelet lipidome. Associations among lipid changes, clinical data, and plasma biomarkers of coagulopathy and inflammation were explored.RESULTS: More than 60% of the annotated platelet lipids were significantly altered in septic shock. Cholesteryl esters, sphingomyelins, lysophosphatidylcholines, and ether-lipids were significantly reduced, while ceramide levels increased. Fatty acyl chain remodeling displayed distinct patterns, with polyunsaturated fatty acids increasing in triacylglycerols and decreasing in phospholipids. Lipid alterations were strongly associated with thrombocytopenia, and lysophosphatidylcholine levels inversely correlated with disease severity, as indicated by the Sequential Organ Failure Assessment score.CONCLUSIONS: Septic shock induces significant disruptions in the platelet lipidome, with the extent of these alterations correlating with sepsis-associated thrombocytopenia severity. The observed changes affect multiple lipid classes, surpassing those reported under physiological conditions or in other diseases. These findings highlight the impact of sepsis-driven dysregulated inflammation and coagulopathy on platelet lipid composition, providing new insights into sepsis pathophysiology.

KW - blood platelet

KW - coagulopathy

KW - inflammation

KW - lipidomics

KW - septic shock

UR - https://www.scopus.com/pages/publications/105027660052

U2 - 10.1016/j.rpth.2025.103332

DO - 10.1016/j.rpth.2025.103332

M3 - Article

C2 - 41624233

SN - 2475-0379

VL - 10

SP - 103332

JO - Research and Practice in Thrombosis and Haemostasis

JF - Research and Practice in Thrombosis and Haemostasis

IS - 1

M1 - 103332

ER -

Platelet lipidome alterations in septic shock: a matched case-control study

Résumé

Financement

SDG des Nations Unies

Accès au document

Autres fichiers et liens

Empreinte digitale

Presse/médias

Catholic University Louvain (UCLouvain) Researchers Report on Findings in Sepsis (Platelet lipidome alterations in septic shock: a matched case-control study)

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