Plant extract-based diets differently modulate immune responses and resistance to bacterial infection in striped catfish (Pangasianodon hypophthalmus)

Truong Quynh Nhu, Bui Thi Bich Hang, Le Thi Bach, Bui Thi Buu Hue, Joëlle Quetin-Leclercq, Marie Louise Scippo, Nguyen Thanh Phuong, Patrick Kestemont

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Résumé

A feeding trial was performed to compare the effects of five ethanol herbal extracts (bhumi amla, Phyllanthus amarus Schum and Thonn [Pa]; guava, Psidium guajava L. [Pg]; sensitive plant, Mimosa pudica L. [Mp]; neem, Azadirachta indica A. Juss [Ai] and asthma plant, Euphorbia hirta L. [Eh]) on the immune response and disease resistance against Edwardsiella ictaluri infection of striped catfish (Pangasianodon hypophthalmus). Fish were fed diets supplemented with two doses of each plant extract (0% [basal diet], 0.4% Eh [Eh0.4], 2.0% Eh [Eh2.0], 0.2% Pa [Pa0.2], 1.0% Pa [Pa1.0], 0.2% Pg [Pg0.2], 1.0% Pg [Pg1.0], 0.4% Mp [Mp0.4], 2.0% Mp [Mp2.0], 0.4% Ai [Ai0.4], 2.0% Ai [Ai2.0]) for 8 weeks. Results showed that hematological parameters (total red blood cells, white blood cells, lymphocytes, monocytes, and neutrophils) of fish fed extract-based diets were significantly higher than in those fed the control diet (p < 0.05) after 4 and 8 weeks. Plasma lysozyme activity increased in fish whose diets contained both doses of Eh (p < 0.05) in week 4 (W4), whereas lysozyme activity increased in fish fed 0.2% Pa and Pg, and 2.0% Ai and Eh (p < 0.05) in week 8 (W8). The lysozyme levels in skin mucus did not significantly differ between treatments (p > 0.05) in W4 and after the bacterial challenge test. At the end of the feeding trial, levels of ACH50 significantly increased in most of extract groups compared to the control group (p < 0.05). Total immunoglobulin increased considerably in both the plasma and skin mucus of fish fed extract-supplemented diets after 8 weeks. In addition, dietary supplementation with Pg, Mp, Pa0.2, Eh2.0, and Ai0.4 for 8 weeks considerably reduced the cumulative mortality against E. ictaluri infection in striped catfish. The results suggest that plant extracts possibly modulate the striped catfish immune response in a time and dose dependent manner. Specifically, diets enriched with extracts of P. guajava at 0.2 and 1.0%, or M. pudica at 2.0% for 8 weeks, have great potential for improving striped catfish health by enhancing the immune system and reducing mortality against bacterial challenges.

langue originaleAnglais
Pages (de - à)913-924
Nombre de pages12
journalFish and Shellfish Immunology
Volume92
Les DOIs
Etat de la publicationPublié - 1 sept. 2019

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  • Thèses de l'étudiant

    Immunomodulatory potential of different herbal plant extracts on striped catfish (Pangasianodon hypophthalmus): in vitro and in vivo approaches

    Author: Truong Quynh Nhu, X., 19 déc. 2019

    Superviseur: Kestemont, P. (Promoteur), Bich Hang, B. T. (Personne externe) (Copromoteur), De Laender, F. (Président), Quetin-Leclercq, J. (Personne externe) (Jury), SCIPPO, M. L. (Personne externe) (Jury), Mandiki, R. (Jury) & Torrecillas, S. (Personne externe) (Jury)

    Thèse de l'étudiant: Doc typesDocteur en Sciences

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