Physiological and proteomic responses to corticosteroid treatments in Eurasian perch, Perca fluviatilis: Investigation of immune-related parameters

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Résumé

The comparative effects of cortisol and 11-deoxycorticosterone (DOC), two major corticosteroids in fish, have yet received little attention in teleosts. We evaluated the proteomic and immune responses of Eurasian perch to chronic corticosteroid treatments. We implanted immature perch with cortisol (80 mg/kg) or DOC (4 mg/kg) and measured the proportions of blood leucocytes, immune indices in the plasma, spleen and liver (complement and lysozyme activity, total immunoglobulin and immune gene expression in the tissues) and differential proteome expression (corticosteroid versus control) in the liver and the spleen on days 2, 4 and 14 post-treatment. Implantation of cortisol decreased the ratio of blood leucocytes and depressed Ig levels in both organs while DOC modulated the proportion of leucocyte sub-populations (increase in lymphocytes and decrease in granulocytes). In contrast, the innate humoral immunity was not strongly influenced by any of corticosteroid implants. The only immune parameter that was significantly affected was lysozyme, after DOC treatment. A number of proteins were differentially regulated by these hormones and some were identified in the liver (21 for cortisol and 8 for DOC) and in the spleen (10 for cortisol and 10 for DOC). None of the proteins was directly linked to immunity, except the natural killer enhancing factor, which was repressed by cortisol in the spleen. Our results also confirm that the proteins involved in energetic and glucose metabolism are affected by corticosteroids. Furthermore, these corticosteroids differently regulate immune status in Eurasian perch and they primarily impact leucocytes, as opposed to innate immune function.

langue originaleAnglais
Pages (de - à)86-98
Nombre de pages13
journalComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume25
Les DOIs
étatPublié - 1 mars 2018

Empreinte digitale

Perches
Desoxycorticosterone
Proteomics
Hydrocortisone
Adrenal Cortex Hormones
Leukocytes
Spleen
Liver
Muramidase
Innate Immunity
Blood
Immunoglobulin Genes
Proteins
Lymphocytes
Proteome
Humoral Immunity
Granulocytes
Metabolism
Gene expression
Fish

Citer ceci

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title = "Physiological and proteomic responses to corticosteroid treatments in Eurasian perch, Perca fluviatilis: Investigation of immune-related parameters",
abstract = "The comparative effects of cortisol and 11-deoxycorticosterone (DOC), two major corticosteroids in fish, have yet received little attention in teleosts. We evaluated the proteomic and immune responses of Eurasian perch to chronic corticosteroid treatments. We implanted immature perch with cortisol (80 mg/kg) or DOC (4 mg/kg) and measured the proportions of blood leucocytes, immune indices in the plasma, spleen and liver (complement and lysozyme activity, total immunoglobulin and immune gene expression in the tissues) and differential proteome expression (corticosteroid versus control) in the liver and the spleen on days 2, 4 and 14 post-treatment. Implantation of cortisol decreased the ratio of blood leucocytes and depressed Ig levels in both organs while DOC modulated the proportion of leucocyte sub-populations (increase in lymphocytes and decrease in granulocytes). In contrast, the innate humoral immunity was not strongly influenced by any of corticosteroid implants. The only immune parameter that was significantly affected was lysozyme, after DOC treatment. A number of proteins were differentially regulated by these hormones and some were identified in the liver (21 for cortisol and 8 for DOC) and in the spleen (10 for cortisol and 10 for DOC). None of the proteins was directly linked to immunity, except the natural killer enhancing factor, which was repressed by cortisol in the spleen. Our results also confirm that the proteins involved in energetic and glucose metabolism are affected by corticosteroids. Furthermore, these corticosteroids differently regulate immune status in Eurasian perch and they primarily impact leucocytes, as opposed to innate immune function.",
keywords = "Corticosteroids, Eurasian perch, Glucose metabolism, Immunity, Proteomics",
author = "Sylvain Milla and Sophie Massart and C{\'e}dric Mathieu and Neil Wang and Caroline Douny and Jessica Douxfils and Scippo, {Marie Louise} and {De Pauw}, Edwin and Marc Dieu and Fr{\'e}d{\'e}ric Silvestre and Patrick Kestemont",
year = "2018",
month = "3",
day = "1",
doi = "10.1016/j.cbd.2017.11.005",
language = "English",
volume = "25",
pages = "86--98",
journal = "Comparative Biochemistry and Physiology. Part D. Genomics and Proteomics",
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TY - JOUR

T1 - Physiological and proteomic responses to corticosteroid treatments in Eurasian perch, Perca fluviatilis

T2 - Investigation of immune-related parameters

AU - Milla, Sylvain

AU - Massart, Sophie

AU - Mathieu, Cédric

AU - Wang, Neil

AU - Douny, Caroline

AU - Douxfils, Jessica

AU - Scippo, Marie Louise

AU - De Pauw, Edwin

AU - Dieu, Marc

AU - Silvestre, Frédéric

AU - Kestemont, Patrick

PY - 2018/3/1

Y1 - 2018/3/1

N2 - The comparative effects of cortisol and 11-deoxycorticosterone (DOC), two major corticosteroids in fish, have yet received little attention in teleosts. We evaluated the proteomic and immune responses of Eurasian perch to chronic corticosteroid treatments. We implanted immature perch with cortisol (80 mg/kg) or DOC (4 mg/kg) and measured the proportions of blood leucocytes, immune indices in the plasma, spleen and liver (complement and lysozyme activity, total immunoglobulin and immune gene expression in the tissues) and differential proteome expression (corticosteroid versus control) in the liver and the spleen on days 2, 4 and 14 post-treatment. Implantation of cortisol decreased the ratio of blood leucocytes and depressed Ig levels in both organs while DOC modulated the proportion of leucocyte sub-populations (increase in lymphocytes and decrease in granulocytes). In contrast, the innate humoral immunity was not strongly influenced by any of corticosteroid implants. The only immune parameter that was significantly affected was lysozyme, after DOC treatment. A number of proteins were differentially regulated by these hormones and some were identified in the liver (21 for cortisol and 8 for DOC) and in the spleen (10 for cortisol and 10 for DOC). None of the proteins was directly linked to immunity, except the natural killer enhancing factor, which was repressed by cortisol in the spleen. Our results also confirm that the proteins involved in energetic and glucose metabolism are affected by corticosteroids. Furthermore, these corticosteroids differently regulate immune status in Eurasian perch and they primarily impact leucocytes, as opposed to innate immune function.

AB - The comparative effects of cortisol and 11-deoxycorticosterone (DOC), two major corticosteroids in fish, have yet received little attention in teleosts. We evaluated the proteomic and immune responses of Eurasian perch to chronic corticosteroid treatments. We implanted immature perch with cortisol (80 mg/kg) or DOC (4 mg/kg) and measured the proportions of blood leucocytes, immune indices in the plasma, spleen and liver (complement and lysozyme activity, total immunoglobulin and immune gene expression in the tissues) and differential proteome expression (corticosteroid versus control) in the liver and the spleen on days 2, 4 and 14 post-treatment. Implantation of cortisol decreased the ratio of blood leucocytes and depressed Ig levels in both organs while DOC modulated the proportion of leucocyte sub-populations (increase in lymphocytes and decrease in granulocytes). In contrast, the innate humoral immunity was not strongly influenced by any of corticosteroid implants. The only immune parameter that was significantly affected was lysozyme, after DOC treatment. A number of proteins were differentially regulated by these hormones and some were identified in the liver (21 for cortisol and 8 for DOC) and in the spleen (10 for cortisol and 10 for DOC). None of the proteins was directly linked to immunity, except the natural killer enhancing factor, which was repressed by cortisol in the spleen. Our results also confirm that the proteins involved in energetic and glucose metabolism are affected by corticosteroids. Furthermore, these corticosteroids differently regulate immune status in Eurasian perch and they primarily impact leucocytes, as opposed to innate immune function.

KW - Corticosteroids

KW - Eurasian perch

KW - Glucose metabolism

KW - Immunity

KW - Proteomics

UR - http://www.scopus.com/inward/record.url?scp=85037622523&partnerID=8YFLogxK

U2 - 10.1016/j.cbd.2017.11.005

DO - 10.1016/j.cbd.2017.11.005

M3 - Article

AN - SCOPUS:85037622523

VL - 25

SP - 86

EP - 98

JO - Comparative Biochemistry and Physiology. Part D. Genomics and Proteomics

JF - Comparative Biochemistry and Physiology. Part D. Genomics and Proteomics

SN - 1744-117X

ER -