Résumé
The comparative effects of cortisol and 11-deoxycorticosterone (DOC), two major corticosteroids in fish, have yet received little attention in teleosts. We evaluated the proteomic and immune responses of Eurasian perch to chronic corticosteroid treatments. We implanted immature perch with cortisol (80 mg/kg) or DOC (4 mg/kg) and measured the proportions of blood leucocytes, immune indices in the plasma, spleen and liver (complement and lysozyme activity, total immunoglobulin and immune gene expression in the tissues) and differential proteome expression (corticosteroid versus control) in the liver and the spleen on days 2, 4 and 14 post-treatment. Implantation of cortisol decreased the ratio of blood leucocytes and depressed Ig levels in both organs while DOC modulated the proportion of leucocyte sub-populations (increase in lymphocytes and decrease in granulocytes). In contrast, the innate humoral immunity was not strongly influenced by any of corticosteroid implants. The only immune parameter that was significantly affected was lysozyme, after DOC treatment. A number of proteins were differentially regulated by these hormones and some were identified in the liver (21 for cortisol and 8 for DOC) and in the spleen (10 for cortisol and 10 for DOC). None of the proteins was directly linked to immunity, except the natural killer enhancing factor, which was repressed by cortisol in the spleen. Our results also confirm that the proteins involved in energetic and glucose metabolism are affected by corticosteroids. Furthermore, these corticosteroids differently regulate immune status in Eurasian perch and they primarily impact leucocytes, as opposed to innate immune function.
| langue originale | Anglais |
|---|---|
| Pages (de - à) | 86-98 |
| Nombre de pages | 13 |
| journal | Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics |
| Volume | 25 |
| Les DOIs | |
| état | Publié - 1 mars 2018 |
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Physiological and proteomic responses to corticosteroid treatments in Eurasian perch, Perca fluviatilis : Investigation of immune-related parameters. / Milla, Sylvain; Massart, Sophie; Mathieu, Cédric; Wang, Neil; Douny, Caroline; Douxfils, Jessica; Scippo, Marie Louise; De Pauw, Edwin; Dieu, Marc; Silvestre, Frédéric; Kestemont, Patrick.
Dans: Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, Vol 25, 01.03.2018, p. 86-98.Résultats de recherche: Contribution à un journal/une revue › Article
TY - JOUR
T1 - Physiological and proteomic responses to corticosteroid treatments in Eurasian perch, Perca fluviatilis
T2 - Investigation of immune-related parameters
AU - Milla, Sylvain
AU - Massart, Sophie
AU - Mathieu, Cédric
AU - Wang, Neil
AU - Douny, Caroline
AU - Douxfils, Jessica
AU - Scippo, Marie Louise
AU - De Pauw, Edwin
AU - Dieu, Marc
AU - Silvestre, Frédéric
AU - Kestemont, Patrick
PY - 2018/3/1
Y1 - 2018/3/1
N2 - The comparative effects of cortisol and 11-deoxycorticosterone (DOC), two major corticosteroids in fish, have yet received little attention in teleosts. We evaluated the proteomic and immune responses of Eurasian perch to chronic corticosteroid treatments. We implanted immature perch with cortisol (80 mg/kg) or DOC (4 mg/kg) and measured the proportions of blood leucocytes, immune indices in the plasma, spleen and liver (complement and lysozyme activity, total immunoglobulin and immune gene expression in the tissues) and differential proteome expression (corticosteroid versus control) in the liver and the spleen on days 2, 4 and 14 post-treatment. Implantation of cortisol decreased the ratio of blood leucocytes and depressed Ig levels in both organs while DOC modulated the proportion of leucocyte sub-populations (increase in lymphocytes and decrease in granulocytes). In contrast, the innate humoral immunity was not strongly influenced by any of corticosteroid implants. The only immune parameter that was significantly affected was lysozyme, after DOC treatment. A number of proteins were differentially regulated by these hormones and some were identified in the liver (21 for cortisol and 8 for DOC) and in the spleen (10 for cortisol and 10 for DOC). None of the proteins was directly linked to immunity, except the natural killer enhancing factor, which was repressed by cortisol in the spleen. Our results also confirm that the proteins involved in energetic and glucose metabolism are affected by corticosteroids. Furthermore, these corticosteroids differently regulate immune status in Eurasian perch and they primarily impact leucocytes, as opposed to innate immune function.
AB - The comparative effects of cortisol and 11-deoxycorticosterone (DOC), two major corticosteroids in fish, have yet received little attention in teleosts. We evaluated the proteomic and immune responses of Eurasian perch to chronic corticosteroid treatments. We implanted immature perch with cortisol (80 mg/kg) or DOC (4 mg/kg) and measured the proportions of blood leucocytes, immune indices in the plasma, spleen and liver (complement and lysozyme activity, total immunoglobulin and immune gene expression in the tissues) and differential proteome expression (corticosteroid versus control) in the liver and the spleen on days 2, 4 and 14 post-treatment. Implantation of cortisol decreased the ratio of blood leucocytes and depressed Ig levels in both organs while DOC modulated the proportion of leucocyte sub-populations (increase in lymphocytes and decrease in granulocytes). In contrast, the innate humoral immunity was not strongly influenced by any of corticosteroid implants. The only immune parameter that was significantly affected was lysozyme, after DOC treatment. A number of proteins were differentially regulated by these hormones and some were identified in the liver (21 for cortisol and 8 for DOC) and in the spleen (10 for cortisol and 10 for DOC). None of the proteins was directly linked to immunity, except the natural killer enhancing factor, which was repressed by cortisol in the spleen. Our results also confirm that the proteins involved in energetic and glucose metabolism are affected by corticosteroids. Furthermore, these corticosteroids differently regulate immune status in Eurasian perch and they primarily impact leucocytes, as opposed to innate immune function.
KW - Corticosteroids
KW - Eurasian perch
KW - Glucose metabolism
KW - Immunity
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=85037622523&partnerID=8YFLogxK
U2 - 10.1016/j.cbd.2017.11.005
DO - 10.1016/j.cbd.2017.11.005
M3 - Article
AN - SCOPUS:85037622523
VL - 25
SP - 86
EP - 98
JO - Comparative Biochemistry and Physiology. Part D. Genomics and Proteomics
JF - Comparative Biochemistry and Physiology. Part D. Genomics and Proteomics
SN - 1744-117X
ER -