TY - JOUR
T1 - PBP2b plays a key role in both peripheral growth and septum positioning in Lactococcus lactis
AU - David, Blandine
AU - Duchêne, Marie-Clémence
AU - Haustenne, Gabrielle Laurie
AU - Pérez-Núñez, Daniel
AU - Chapot-Chartier, Marie-Pierre
AU - De Bolle, Xavier
AU - Guédon, Eric
AU - Hols, Pascal
AU - Hallet, Bernard
N1 - Funding Information:
This work was supported by Fonds Spéciaux de la Recherche (FSR), Université catholique de Louvain (UCL, https://uclouvain.be/fr/ index.html) to PH; Fonds National de Recherche Scientifique (FNRS-FRS, www.fnrs.be), Grant number 2.4.620.07 to PH; Actions de Recherche Concertées (ARC), Université catholique de Louvain (UCL, https://uclouvain.be/fr/index.html) and Communauté Française de Belgique, Grant numbers 07/12-004 and 012/17-046 to PH. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank S. Kulakauskas for the gift of pbp disruption plasmids. We warmly thank E. Bernard and T. Rolain for helpful discussions and M. Deghorain for critical reading of the manuscript. PH and BH are Senior Research Associate and Honorary Research Associate at the FNRS, respectively.
Publisher Copyright:
© 2018 David et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2018/5/1
Y1 - 2018/5/1
N2 - Lactococcus lactis is an ovoid bacterium that forms filaments during planktonic and biofilm lifestyles by uncoupling cell division from cell elongation. In this work, we investigate the role of the leading peptidoglycan synthase PBP2b that is dedicated to cell elongation in ovococci. We show that the localization of a fluorescent derivative of PBP2b remains associated to the septal region and superimposed with structural changes of FtsZ during both vegetative growth and filamentation indicating that PBP2b remains intimately associated to the division machinery during the whole cell cycle. In addition, we show that PBP2b-negative cells of L. lactis are not only defective in peripheral growth; they are also affected in septum positioning. This septation defect does not simply result from the absence of the protein in the cell growth machinery since it is also observed when PBP2b-deficient cells are complemented by a catalytically inactive variant of PBP2b. Finally, we show that round cells resulting from β-lactam treatment are not altered in septation, suggesting that shape elongation as such is not a major determinant for selection of the division site. Altogether, we propose that the specific PBP2b transpeptidase activity at the septum plays an important role for tagging future division sites during L. lactis cell cycle.
AB - Lactococcus lactis is an ovoid bacterium that forms filaments during planktonic and biofilm lifestyles by uncoupling cell division from cell elongation. In this work, we investigate the role of the leading peptidoglycan synthase PBP2b that is dedicated to cell elongation in ovococci. We show that the localization of a fluorescent derivative of PBP2b remains associated to the septal region and superimposed with structural changes of FtsZ during both vegetative growth and filamentation indicating that PBP2b remains intimately associated to the division machinery during the whole cell cycle. In addition, we show that PBP2b-negative cells of L. lactis are not only defective in peripheral growth; they are also affected in septum positioning. This septation defect does not simply result from the absence of the protein in the cell growth machinery since it is also observed when PBP2b-deficient cells are complemented by a catalytically inactive variant of PBP2b. Finally, we show that round cells resulting from β-lactam treatment are not altered in septation, suggesting that shape elongation as such is not a major determinant for selection of the division site. Altogether, we propose that the specific PBP2b transpeptidase activity at the septum plays an important role for tagging future division sites during L. lactis cell cycle.
UR - http://www.scopus.com/inward/record.url?scp=85047481422&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0198014
DO - 10.1371/journal.pone.0198014
M3 - Article
C2 - 29791496
SN - 1932-6203
VL - 13
SP - e0198014
JO - PLoS ONE
JF - PLoS ONE
IS - 5
M1 - e0198014
ER -