OXA-427, a new plasmid-borne carbapenem-hydrolysing class D β-lactamase in Enterobacteriaceae

Pierre Bogaerts, Thierry Naas, Veroniek Saegeman, Remy A. Bonnin, Annette Schuermans, Stéphanie Evrard, Warda Bouchahrouf, Thomas Jove, Didier Tande, Xavier de Bolle, Te Din Huang, Laurent Dortet, Youri Glupczynski

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

Objectives: To describe a novel plasmid-borne class D carbapenemase (CHDL) named OXA-427 identified in several Enterobacteriaceae clinical isolates from nine patients in one Belgian hospital. Methods: OXA-427-producing isolates were analysed by an electrochemical imipenem hydrolysis method (BYG Carba test), Carba NP test, conventional phenotypic assays and by molecular methods (PCR, whole sequencing of the OXA-427-encoding plasmid and cloning). The antimicrobial resistance profile of OXA-427 was analysed by expression of the cloned gene in Escherichia coli DH10B and J53. Results: Eleven OXA-427-producing Enterobacteriaceae isolates of various species were identified from clinical specimens of nine patients between March 2012 and June 2014. OXA-427 shares only 22%-29% amino acid identity with OXA-48-like enzymes and other acquired CHDL (e.g. OXA-23, -24/40 and -58 of Acinetobacter spp.). Conversely, it appeared closely related to the chromosomal class D β-lactamase of Aeromonas media, Aeromonas hydrophila and Aeromonas sobria (99%, 89% and 77% of identity, respectively). When expressed in E. coli, OXA-427 hydrolysed imipenem and conferred resistance to extended-spectrum cephalosporins (mostly ceftazidime), penicillins including temocillin, and reduced susceptibility to carbapenems. The blaOXA-427 gene was located in a 45 kb resistance island on a 177 kb IncA/C plasmid. Conclusions: OXA-427 is a novel CHDL most closely related to chromosomal class D β-lactamase of A. media WS. It confers resistance to penicillins, ceftazidime and aztreonam and in some instances to carbapenems. OXA- 427, which is not detectable by classical molecular tests, caused a protracted outbreak in one university hospital over a 2 year period.

langueAnglais
Pages2469-2477
Nombre de pages9
journalThe Journal of antimicrobial chemotherapy
Volume72
Numéro9
Les DOIs
étatPublié - 1 sept. 2017

Empreinte digitale

Carbapenems
Enterobacteriaceae
beta-Lactamases
Plasmids
Aeromonas
Ceftazidime
Imipenem
Asp(5)-oxytocin
Escherichia coli
Penicillin Resistance
Aeromonas hydrophila
Aztreonam
Acinetobacter
Cephalosporins
Islands
Penicillins
Disease Outbreaks
Organism Cloning
Hydrolysis

mots-clés

    Citer ceci

    Bogaerts, P., Naas, T., Saegeman, V., Bonnin, R. A., Schuermans, A., Evrard, S., ... Glupczynski, Y. (2017). OXA-427, a new plasmid-borne carbapenem-hydrolysing class D β-lactamase in Enterobacteriaceae. DOI: 10.1093/jac/dkx184
    Bogaerts, Pierre ; Naas, Thierry ; Saegeman, Veroniek ; Bonnin, Remy A. ; Schuermans, Annette ; Evrard, Stéphanie ; Bouchahrouf, Warda ; Jove, Thomas ; Tande, Didier ; de Bolle, Xavier ; Huang, Te Din ; Dortet, Laurent ; Glupczynski, Youri. / OXA-427, a new plasmid-borne carbapenem-hydrolysing class D β-lactamase in Enterobacteriaceae. Dans: The Journal of antimicrobial chemotherapy. 2017 ; Vol 72, Numéro 9. p. 2469-2477
    @article{01de9e11284246df9a4794d5573600f1,
    title = "OXA-427, a new plasmid-borne carbapenem-hydrolysing class D β-lactamase in Enterobacteriaceae",
    abstract = "Objectives: To describe a novel plasmid-borne class D carbapenemase (CHDL) named OXA-427 identified in several Enterobacteriaceae clinical isolates from nine patients in one Belgian hospital. Methods: OXA-427-producing isolates were analysed by an electrochemical imipenem hydrolysis method (BYG Carba test), Carba NP test, conventional phenotypic assays and by molecular methods (PCR, whole sequencing of the OXA-427-encoding plasmid and cloning). The antimicrobial resistance profile of OXA-427 was analysed by expression of the cloned gene in Escherichia coli DH10B and J53. Results: Eleven OXA-427-producing Enterobacteriaceae isolates of various species were identified from clinical specimens of nine patients between March 2012 and June 2014. OXA-427 shares only 22{\%}-29{\%} amino acid identity with OXA-48-like enzymes and other acquired CHDL (e.g. OXA-23, -24/40 and -58 of Acinetobacter spp.). Conversely, it appeared closely related to the chromosomal class D β-lactamase of Aeromonas media, Aeromonas hydrophila and Aeromonas sobria (99{\%}, 89{\%} and 77{\%} of identity, respectively). When expressed in E. coli, OXA-427 hydrolysed imipenem and conferred resistance to extended-spectrum cephalosporins (mostly ceftazidime), penicillins including temocillin, and reduced susceptibility to carbapenems. The blaOXA-427 gene was located in a 45 kb resistance island on a 177 kb IncA/C plasmid. Conclusions: OXA-427 is a novel CHDL most closely related to chromosomal class D β-lactamase of A. media WS. It confers resistance to penicillins, ceftazidime and aztreonam and in some instances to carbapenems. OXA- 427, which is not detectable by classical molecular tests, caused a protracted outbreak in one university hospital over a 2 year period.",
    keywords = "Journal Article",
    author = "Pierre Bogaerts and Thierry Naas and Veroniek Saegeman and Bonnin, {Remy A.} and Annette Schuermans and St{\'e}phanie Evrard and Warda Bouchahrouf and Thomas Jove and Didier Tande and {de Bolle}, Xavier and Huang, {Te Din} and Laurent Dortet and Youri Glupczynski",
    year = "2017",
    month = "9",
    day = "1",
    doi = "10.1093/jac/dkx184",
    language = "English",
    volume = "72",
    pages = "2469--2477",
    journal = "The Journal of antimicrobial chemotherapy",
    issn = "0305-7453",
    publisher = "Oxford University Press",
    number = "9",

    }

    Bogaerts, P, Naas, T, Saegeman, V, Bonnin, RA, Schuermans, A, Evrard, S, Bouchahrouf, W, Jove, T, Tande, D, de Bolle, X, Huang, TD, Dortet, L & Glupczynski, Y 2017, 'OXA-427, a new plasmid-borne carbapenem-hydrolysing class D β-lactamase in Enterobacteriaceae' The Journal of antimicrobial chemotherapy, VOL. 72, Numéro 9, p. 2469-2477. DOI: 10.1093/jac/dkx184

    OXA-427, a new plasmid-borne carbapenem-hydrolysing class D β-lactamase in Enterobacteriaceae. / Bogaerts, Pierre; Naas, Thierry; Saegeman, Veroniek; Bonnin, Remy A.; Schuermans, Annette; Evrard, Stéphanie; Bouchahrouf, Warda; Jove, Thomas; Tande, Didier; de Bolle, Xavier; Huang, Te Din; Dortet, Laurent; Glupczynski, Youri.

    Dans: The Journal of antimicrobial chemotherapy, Vol 72, Numéro 9, 01.09.2017, p. 2469-2477.

    Résultats de recherche: Contribution à un journal/une revueArticle

    TY - JOUR

    T1 - OXA-427, a new plasmid-borne carbapenem-hydrolysing class D β-lactamase in Enterobacteriaceae

    AU - Bogaerts,Pierre

    AU - Naas,Thierry

    AU - Saegeman,Veroniek

    AU - Bonnin,Remy A.

    AU - Schuermans,Annette

    AU - Evrard,Stéphanie

    AU - Bouchahrouf,Warda

    AU - Jove,Thomas

    AU - Tande,Didier

    AU - de Bolle,Xavier

    AU - Huang,Te Din

    AU - Dortet,Laurent

    AU - Glupczynski,Youri

    PY - 2017/9/1

    Y1 - 2017/9/1

    N2 - Objectives: To describe a novel plasmid-borne class D carbapenemase (CHDL) named OXA-427 identified in several Enterobacteriaceae clinical isolates from nine patients in one Belgian hospital. Methods: OXA-427-producing isolates were analysed by an electrochemical imipenem hydrolysis method (BYG Carba test), Carba NP test, conventional phenotypic assays and by molecular methods (PCR, whole sequencing of the OXA-427-encoding plasmid and cloning). The antimicrobial resistance profile of OXA-427 was analysed by expression of the cloned gene in Escherichia coli DH10B and J53. Results: Eleven OXA-427-producing Enterobacteriaceae isolates of various species were identified from clinical specimens of nine patients between March 2012 and June 2014. OXA-427 shares only 22%-29% amino acid identity with OXA-48-like enzymes and other acquired CHDL (e.g. OXA-23, -24/40 and -58 of Acinetobacter spp.). Conversely, it appeared closely related to the chromosomal class D β-lactamase of Aeromonas media, Aeromonas hydrophila and Aeromonas sobria (99%, 89% and 77% of identity, respectively). When expressed in E. coli, OXA-427 hydrolysed imipenem and conferred resistance to extended-spectrum cephalosporins (mostly ceftazidime), penicillins including temocillin, and reduced susceptibility to carbapenems. The blaOXA-427 gene was located in a 45 kb resistance island on a 177 kb IncA/C plasmid. Conclusions: OXA-427 is a novel CHDL most closely related to chromosomal class D β-lactamase of A. media WS. It confers resistance to penicillins, ceftazidime and aztreonam and in some instances to carbapenems. OXA- 427, which is not detectable by classical molecular tests, caused a protracted outbreak in one university hospital over a 2 year period.

    AB - Objectives: To describe a novel plasmid-borne class D carbapenemase (CHDL) named OXA-427 identified in several Enterobacteriaceae clinical isolates from nine patients in one Belgian hospital. Methods: OXA-427-producing isolates were analysed by an electrochemical imipenem hydrolysis method (BYG Carba test), Carba NP test, conventional phenotypic assays and by molecular methods (PCR, whole sequencing of the OXA-427-encoding plasmid and cloning). The antimicrobial resistance profile of OXA-427 was analysed by expression of the cloned gene in Escherichia coli DH10B and J53. Results: Eleven OXA-427-producing Enterobacteriaceae isolates of various species were identified from clinical specimens of nine patients between March 2012 and June 2014. OXA-427 shares only 22%-29% amino acid identity with OXA-48-like enzymes and other acquired CHDL (e.g. OXA-23, -24/40 and -58 of Acinetobacter spp.). Conversely, it appeared closely related to the chromosomal class D β-lactamase of Aeromonas media, Aeromonas hydrophila and Aeromonas sobria (99%, 89% and 77% of identity, respectively). When expressed in E. coli, OXA-427 hydrolysed imipenem and conferred resistance to extended-spectrum cephalosporins (mostly ceftazidime), penicillins including temocillin, and reduced susceptibility to carbapenems. The blaOXA-427 gene was located in a 45 kb resistance island on a 177 kb IncA/C plasmid. Conclusions: OXA-427 is a novel CHDL most closely related to chromosomal class D β-lactamase of A. media WS. It confers resistance to penicillins, ceftazidime and aztreonam and in some instances to carbapenems. OXA- 427, which is not detectable by classical molecular tests, caused a protracted outbreak in one university hospital over a 2 year period.

    KW - Journal Article

    UR - http://www.scopus.com/inward/record.url?scp=85039982659&partnerID=8YFLogxK

    U2 - 10.1093/jac/dkx184

    DO - 10.1093/jac/dkx184

    M3 - Article

    VL - 72

    SP - 2469

    EP - 2477

    JO - The Journal of antimicrobial chemotherapy

    T2 - The Journal of antimicrobial chemotherapy

    JF - The Journal of antimicrobial chemotherapy

    SN - 0305-7453

    IS - 9

    ER -

    Bogaerts P, Naas T, Saegeman V, Bonnin RA, Schuermans A, Evrard S et al. OXA-427, a new plasmid-borne carbapenem-hydrolysing class D β-lactamase in Enterobacteriaceae. The Journal of antimicrobial chemotherapy. 2017 sept. 1;72(9):2469-2477. Disponible �, DOI: 10.1093/jac/dkx184