OXA-427, a new plasmid-borne carbapenem-hydrolysing class D β-lactamase in Enterobacteriaceae

Pierre Bogaerts, Thierry Naas, Veroniek Saegeman, Remy A Bonnin, Annette Schuermans, Stéphanie Evrard, Warda Bouchahrouf, Thomas Jove, Didier Tande, Xavier de Bolle, Te-Din Huang, Laurent Dortet, Youri Glupczynski

Résultats de recherche: Recherche - Revue par des pairsArticle

Résumé

Objectives: To describe a novel plasmid-borne class D carbapenemase (CHDL) named OXA-427 identified in several Enterobacteriaceae clinical isolates from nine patients in one Belgian hospital.

Methods: OXA-427-producing isolates were analysed by an electrochemical imipenem hydrolysis method (BYG Carba test), Carba NP test, conventional phenotypic assays and by molecular methods (PCR, whole sequencing of the OXA-427-encoding plasmid and cloning). The antimicrobial resistance profile of OXA-427 was analysed by expression of the cloned gene in Escherichia coli DH10B and J53.

Results: Eleven OXA-427-producing Enterobacteriaceae isolates of various species were identified from clinical specimens of nine patients between March 2012 and June 2014. OXA-427 shares only 22%-29% amino acid identity with OXA-48-like enzymes and other acquired CHDL (e.g. OXA-23, -24/40 and -58 of Acinetobacter spp.). Conversely, it appeared closely related to the chromosomal class D β-lactamase of Aeromonas media, Aeromonas hydrophila and Aeromonas sobria (99%, 89% and 77% of identity, respectively). When expressed in E. coli, OXA-427 hydrolysed imipenem and conferred resistance to extended-spectrum cephalosporins (mostly ceftazidime), penicillins including temocillin, and reduced susceptibility to carbapenems. The blaOXA-427 gene was located in a 45 kb resistance island on a 177 kb IncA/C plasmid.

Conclusions: OXA-427 is a novel CHDL most closely related to chromosomal class D β-lactamase of A. media WS. It confers resistance to penicillins, ceftazidime and aztreonam and in some instances to carbapenems. OXA-427, which is not detectable by classical molecular tests, caused a protracted outbreak in one university hospital over a 2 year period.

langueAnglais
Pages2469-2477
Nombre de pages9
journalThe Journal of antimicrobial chemotherapy
Volume72
Numéro9
Les DOIs
étatPublié - 1 sept. 2017
Modification externeOui

Empreinte digitale

Carbapenems
Enterobacteriaceae
beta-Lactamases
Plasmids
Asp(5)-oxytocin
Aeromonas
Ceftazidime
Imipenem
Escherichia coli
Penicillin Resistance
Aeromonas hydrophila
Aztreonam
Acinetobacter
Cephalosporins
Islands
Penicillins
Disease Outbreaks
Organism Cloning
Hydrolysis
Gene Expression

mots-clés

    Citer ceci

    Bogaerts, P., Naas, T., Saegeman, V., Bonnin, R. A., Schuermans, A., Evrard, S., ... Glupczynski, Y. (2017). OXA-427, a new plasmid-borne carbapenem-hydrolysing class D β-lactamase in Enterobacteriaceae. The Journal of antimicrobial chemotherapy, 72(9), 2469-2477. DOI: 10.1093/jac/dkx184
    Bogaerts, Pierre ; Naas, Thierry ; Saegeman, Veroniek ; Bonnin, Remy A ; Schuermans, Annette ; Evrard, Stéphanie ; Bouchahrouf, Warda ; Jove, Thomas ; Tande, Didier ; de Bolle, Xavier ; Huang, Te-Din ; Dortet, Laurent ; Glupczynski, Youri. / OXA-427, a new plasmid-borne carbapenem-hydrolysing class D β-lactamase in Enterobacteriaceae. Dans: The Journal of antimicrobial chemotherapy. 2017 ; Vol 72, Numéro 9. p. 2469-2477
    @article{01de9e11284246df9a4794d5573600f1,
    title = "OXA-427, a new plasmid-borne carbapenem-hydrolysing class D β-lactamase in Enterobacteriaceae",
    abstract = "Objectives: To describe a novel plasmid-borne class D carbapenemase (CHDL) named OXA-427 identified in several Enterobacteriaceae clinical isolates from nine patients in one Belgian hospital.Methods: OXA-427-producing isolates were analysed by an electrochemical imipenem hydrolysis method (BYG Carba test), Carba NP test, conventional phenotypic assays and by molecular methods (PCR, whole sequencing of the OXA-427-encoding plasmid and cloning). The antimicrobial resistance profile of OXA-427 was analysed by expression of the cloned gene in Escherichia coli DH10B and J53.Results: Eleven OXA-427-producing Enterobacteriaceae isolates of various species were identified from clinical specimens of nine patients between March 2012 and June 2014. OXA-427 shares only 22%-29% amino acid identity with OXA-48-like enzymes and other acquired CHDL (e.g. OXA-23, -24/40 and -58 of Acinetobacter spp.). Conversely, it appeared closely related to the chromosomal class D β-lactamase of Aeromonas media, Aeromonas hydrophila and Aeromonas sobria (99%, 89% and 77% of identity, respectively). When expressed in E. coli, OXA-427 hydrolysed imipenem and conferred resistance to extended-spectrum cephalosporins (mostly ceftazidime), penicillins including temocillin, and reduced susceptibility to carbapenems. The blaOXA-427 gene was located in a 45 kb resistance island on a 177 kb IncA/C plasmid.Conclusions: OXA-427 is a novel CHDL most closely related to chromosomal class D β-lactamase of A. media WS. It confers resistance to penicillins, ceftazidime and aztreonam and in some instances to carbapenems. OXA-427, which is not detectable by classical molecular tests, caused a protracted outbreak in one university hospital over a 2 year period.",
    keywords = "Journal Article",
    author = "Pierre Bogaerts and Thierry Naas and Veroniek Saegeman and Bonnin, {Remy A} and Annette Schuermans and Stéphanie Evrard and Warda Bouchahrouf and Thomas Jove and Didier Tande and {de Bolle}, Xavier and Te-Din Huang and Laurent Dortet and Youri Glupczynski",
    year = "2017",
    month = "9",
    doi = "10.1093/jac/dkx184",
    volume = "72",
    pages = "2469--2477",
    journal = "The Journal of antimicrobial chemotherapy",
    issn = "0305-7453",
    publisher = "Oxford University Press",
    number = "9",

    }

    Bogaerts, P, Naas, T, Saegeman, V, Bonnin, RA, Schuermans, A, Evrard, S, Bouchahrouf, W, Jove, T, Tande, D, de Bolle, X, Huang, T-D, Dortet, L & Glupczynski, Y 2017, 'OXA-427, a new plasmid-borne carbapenem-hydrolysing class D β-lactamase in Enterobacteriaceae' The Journal of antimicrobial chemotherapy, VOL 72, Numéro 9, p. 2469-2477. DOI: 10.1093/jac/dkx184

    OXA-427, a new plasmid-borne carbapenem-hydrolysing class D β-lactamase in Enterobacteriaceae. / Bogaerts, Pierre; Naas, Thierry; Saegeman, Veroniek; Bonnin, Remy A; Schuermans, Annette; Evrard, Stéphanie; Bouchahrouf, Warda; Jove, Thomas; Tande, Didier; de Bolle, Xavier; Huang, Te-Din; Dortet, Laurent; Glupczynski, Youri.

    Dans: The Journal of antimicrobial chemotherapy, Vol 72, Numéro 9, 01.09.2017, p. 2469-2477.

    Résultats de recherche: Recherche - Revue par des pairsArticle

    TY - JOUR

    T1 - OXA-427, a new plasmid-borne carbapenem-hydrolysing class D β-lactamase in Enterobacteriaceae

    AU - Bogaerts,Pierre

    AU - Naas,Thierry

    AU - Saegeman,Veroniek

    AU - Bonnin,Remy A

    AU - Schuermans,Annette

    AU - Evrard,Stéphanie

    AU - Bouchahrouf,Warda

    AU - Jove,Thomas

    AU - Tande,Didier

    AU - de Bolle,Xavier

    AU - Huang,Te-Din

    AU - Dortet,Laurent

    AU - Glupczynski,Youri

    PY - 2017/9/1

    Y1 - 2017/9/1

    N2 - Objectives: To describe a novel plasmid-borne class D carbapenemase (CHDL) named OXA-427 identified in several Enterobacteriaceae clinical isolates from nine patients in one Belgian hospital.Methods: OXA-427-producing isolates were analysed by an electrochemical imipenem hydrolysis method (BYG Carba test), Carba NP test, conventional phenotypic assays and by molecular methods (PCR, whole sequencing of the OXA-427-encoding plasmid and cloning). The antimicrobial resistance profile of OXA-427 was analysed by expression of the cloned gene in Escherichia coli DH10B and J53.Results: Eleven OXA-427-producing Enterobacteriaceae isolates of various species were identified from clinical specimens of nine patients between March 2012 and June 2014. OXA-427 shares only 22%-29% amino acid identity with OXA-48-like enzymes and other acquired CHDL (e.g. OXA-23, -24/40 and -58 of Acinetobacter spp.). Conversely, it appeared closely related to the chromosomal class D β-lactamase of Aeromonas media, Aeromonas hydrophila and Aeromonas sobria (99%, 89% and 77% of identity, respectively). When expressed in E. coli, OXA-427 hydrolysed imipenem and conferred resistance to extended-spectrum cephalosporins (mostly ceftazidime), penicillins including temocillin, and reduced susceptibility to carbapenems. The blaOXA-427 gene was located in a 45 kb resistance island on a 177 kb IncA/C plasmid.Conclusions: OXA-427 is a novel CHDL most closely related to chromosomal class D β-lactamase of A. media WS. It confers resistance to penicillins, ceftazidime and aztreonam and in some instances to carbapenems. OXA-427, which is not detectable by classical molecular tests, caused a protracted outbreak in one university hospital over a 2 year period.

    AB - Objectives: To describe a novel plasmid-borne class D carbapenemase (CHDL) named OXA-427 identified in several Enterobacteriaceae clinical isolates from nine patients in one Belgian hospital.Methods: OXA-427-producing isolates were analysed by an electrochemical imipenem hydrolysis method (BYG Carba test), Carba NP test, conventional phenotypic assays and by molecular methods (PCR, whole sequencing of the OXA-427-encoding plasmid and cloning). The antimicrobial resistance profile of OXA-427 was analysed by expression of the cloned gene in Escherichia coli DH10B and J53.Results: Eleven OXA-427-producing Enterobacteriaceae isolates of various species were identified from clinical specimens of nine patients between March 2012 and June 2014. OXA-427 shares only 22%-29% amino acid identity with OXA-48-like enzymes and other acquired CHDL (e.g. OXA-23, -24/40 and -58 of Acinetobacter spp.). Conversely, it appeared closely related to the chromosomal class D β-lactamase of Aeromonas media, Aeromonas hydrophila and Aeromonas sobria (99%, 89% and 77% of identity, respectively). When expressed in E. coli, OXA-427 hydrolysed imipenem and conferred resistance to extended-spectrum cephalosporins (mostly ceftazidime), penicillins including temocillin, and reduced susceptibility to carbapenems. The blaOXA-427 gene was located in a 45 kb resistance island on a 177 kb IncA/C plasmid.Conclusions: OXA-427 is a novel CHDL most closely related to chromosomal class D β-lactamase of A. media WS. It confers resistance to penicillins, ceftazidime and aztreonam and in some instances to carbapenems. OXA-427, which is not detectable by classical molecular tests, caused a protracted outbreak in one university hospital over a 2 year period.

    KW - Journal Article

    U2 - 10.1093/jac/dkx184

    DO - 10.1093/jac/dkx184

    M3 - Article

    VL - 72

    SP - 2469

    EP - 2477

    JO - The Journal of antimicrobial chemotherapy

    T2 - The Journal of antimicrobial chemotherapy

    JF - The Journal of antimicrobial chemotherapy

    SN - 0305-7453

    IS - 9

    ER -

    Bogaerts P, Naas T, Saegeman V, Bonnin RA, Schuermans A, Evrard S et al. OXA-427, a new plasmid-borne carbapenem-hydrolysing class D β-lactamase in Enterobacteriaceae. The Journal of antimicrobial chemotherapy. 2017 sept. 1;72(9):2469-2477. Disponible à, DOI: 10.1093/jac/dkx184