Overview of validated alternative methods for the detection of foodborne bacterial pathogens

Alexander Rohde, Jens Andre Hammerl, Idesbald Boone, Wiebke Jansen, Svenja Fohler, Günter Klein, Ralf Dieckmann, Sascha Al Dahouk

Résultats de recherche: Contribution à un journal/une revueArticle de revue

Résumé

Background Despite huge efforts to combat foodborne bacterial pathogens, the number of foodborne infections remains high throughout the world. Culture-dependent gold standard detection methods with their tedious and time-consuming procedures have approached their limits whereas the use of rapid and user-friendly alternative methods is on the rise. Validation by independent institutions, e.g. AOAC, AFNOR, MicroVal and NordVal, is a key element to demonstrate the applicability of a new method and its equivalence with standard procedures (e.g. DIN, ISO). Scope and approach In this review, the suitability of currently available validated methods for the qualitative and quantitative bacteriological analysis of food is presented and discussed with special emphasis on the method-inherent strengths and weaknesses. Furthermore, an overview on general validation characteristics as well as further promising tools for the detection of foodborne pathogens is given. Key findings and conclusions Improved cultivation methods as well as nucleic acid based and immunological methods dominate the market of alternative methods while emerging techniques like mass spectrometry, microarrays and phage-based techniques have yet to be thoroughly validated. Harmonized validation procedures are highly desirable as well as enhanced efforts to develop validated tests for a greater variety of pathogens, since current validated tests are mainly confined to the detection of Salmonella enterica and Listeria spp.

langue originaleAnglais
Pages (de - à)113-118
Nombre de pages6
journalTrends in Food Science and Technology
Volume62
Les DOIs
étatPublié - 1 avr. 2017

Empreinte digitale

pathogens
methodology
food analysis
Salmonella enterica
Food Analysis
Listeria
qualitative analysis
food pathogens
foodborne illness
Levonorgestrel
bacteriophages
nucleic acids
gold
quantitative analysis
Bacteriophages
Nucleic Acids
testing
mass spectrometry
Mass Spectrometry
markets

Citer ceci

Rohde, Alexander ; Hammerl, Jens Andre ; Boone, Idesbald ; Jansen, Wiebke ; Fohler, Svenja ; Klein, Günter ; Dieckmann, Ralf ; Al Dahouk, Sascha. / Overview of validated alternative methods for the detection of foodborne bacterial pathogens. Dans: Trends in Food Science and Technology. 2017 ; Vol 62. p. 113-118.
@article{2266df0197ed4f0f995ff051c40a3200,
title = "Overview of validated alternative methods for the detection of foodborne bacterial pathogens",
abstract = "Background Despite huge efforts to combat foodborne bacterial pathogens, the number of foodborne infections remains high throughout the world. Culture-dependent gold standard detection methods with their tedious and time-consuming procedures have approached their limits whereas the use of rapid and user-friendly alternative methods is on the rise. Validation by independent institutions, e.g. AOAC, AFNOR, MicroVal and NordVal, is a key element to demonstrate the applicability of a new method and its equivalence with standard procedures (e.g. DIN, ISO). Scope and approach In this review, the suitability of currently available validated methods for the qualitative and quantitative bacteriological analysis of food is presented and discussed with special emphasis on the method-inherent strengths and weaknesses. Furthermore, an overview on general validation characteristics as well as further promising tools for the detection of foodborne pathogens is given. Key findings and conclusions Improved cultivation methods as well as nucleic acid based and immunological methods dominate the market of alternative methods while emerging techniques like mass spectrometry, microarrays and phage-based techniques have yet to be thoroughly validated. Harmonized validation procedures are highly desirable as well as enhanced efforts to develop validated tests for a greater variety of pathogens, since current validated tests are mainly confined to the detection of Salmonella enterica and Listeria spp.",
keywords = "Bacteria, Detection, Foodborne, Pathogen, Validation",
author = "Alexander Rohde and Hammerl, {Jens Andre} and Idesbald Boone and Wiebke Jansen and Svenja Fohler and G{\"u}nter Klein and Ralf Dieckmann and {Al Dahouk}, Sascha",
year = "2017",
month = "4",
day = "1",
doi = "10.1016/j.tifs.2017.02.006",
language = "English",
volume = "62",
pages = "113--118",
journal = "Trends in Food Science and Technology",
issn = "0924-2244",
publisher = "Pergamon Press",

}

Overview of validated alternative methods for the detection of foodborne bacterial pathogens. / Rohde, Alexander; Hammerl, Jens Andre; Boone, Idesbald; Jansen, Wiebke; Fohler, Svenja; Klein, Günter; Dieckmann, Ralf; Al Dahouk, Sascha.

Dans: Trends in Food Science and Technology, Vol 62, 01.04.2017, p. 113-118.

Résultats de recherche: Contribution à un journal/une revueArticle de revue

TY - JOUR

T1 - Overview of validated alternative methods for the detection of foodborne bacterial pathogens

AU - Rohde, Alexander

AU - Hammerl, Jens Andre

AU - Boone, Idesbald

AU - Jansen, Wiebke

AU - Fohler, Svenja

AU - Klein, Günter

AU - Dieckmann, Ralf

AU - Al Dahouk, Sascha

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Background Despite huge efforts to combat foodborne bacterial pathogens, the number of foodborne infections remains high throughout the world. Culture-dependent gold standard detection methods with their tedious and time-consuming procedures have approached their limits whereas the use of rapid and user-friendly alternative methods is on the rise. Validation by independent institutions, e.g. AOAC, AFNOR, MicroVal and NordVal, is a key element to demonstrate the applicability of a new method and its equivalence with standard procedures (e.g. DIN, ISO). Scope and approach In this review, the suitability of currently available validated methods for the qualitative and quantitative bacteriological analysis of food is presented and discussed with special emphasis on the method-inherent strengths and weaknesses. Furthermore, an overview on general validation characteristics as well as further promising tools for the detection of foodborne pathogens is given. Key findings and conclusions Improved cultivation methods as well as nucleic acid based and immunological methods dominate the market of alternative methods while emerging techniques like mass spectrometry, microarrays and phage-based techniques have yet to be thoroughly validated. Harmonized validation procedures are highly desirable as well as enhanced efforts to develop validated tests for a greater variety of pathogens, since current validated tests are mainly confined to the detection of Salmonella enterica and Listeria spp.

AB - Background Despite huge efforts to combat foodborne bacterial pathogens, the number of foodborne infections remains high throughout the world. Culture-dependent gold standard detection methods with their tedious and time-consuming procedures have approached their limits whereas the use of rapid and user-friendly alternative methods is on the rise. Validation by independent institutions, e.g. AOAC, AFNOR, MicroVal and NordVal, is a key element to demonstrate the applicability of a new method and its equivalence with standard procedures (e.g. DIN, ISO). Scope and approach In this review, the suitability of currently available validated methods for the qualitative and quantitative bacteriological analysis of food is presented and discussed with special emphasis on the method-inherent strengths and weaknesses. Furthermore, an overview on general validation characteristics as well as further promising tools for the detection of foodborne pathogens is given. Key findings and conclusions Improved cultivation methods as well as nucleic acid based and immunological methods dominate the market of alternative methods while emerging techniques like mass spectrometry, microarrays and phage-based techniques have yet to be thoroughly validated. Harmonized validation procedures are highly desirable as well as enhanced efforts to develop validated tests for a greater variety of pathogens, since current validated tests are mainly confined to the detection of Salmonella enterica and Listeria spp.

KW - Bacteria

KW - Detection

KW - Foodborne

KW - Pathogen

KW - Validation

UR - http://www.scopus.com/inward/record.url?scp=85014239749&partnerID=8YFLogxK

U2 - 10.1016/j.tifs.2017.02.006

DO - 10.1016/j.tifs.2017.02.006

M3 - Review article

AN - SCOPUS:85014239749

VL - 62

SP - 113

EP - 118

JO - Trends in Food Science and Technology

JF - Trends in Food Science and Technology

SN - 0924-2244

ER -