Oligosaccharides successfully thwart hijacking of the salicylic acid pathway by Phytophthora infestans in potato leaves

G. van Aubel; S. Serderidis; J. Ivens; A. Clinckemaillie; A. Legrève; B. Hause; P. Van Cutsem

doi:10.1111/ppa.12908

Oligosaccharides successfully thwart hijacking of the salicylic acid pathway by Phytophthora infestans in potato leaves

G. van Aubel, S. Serderidis, J. Ivens, A. Clinckemaillie, A. Legrève, B. Hause, P. Van Cutsem

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

Résumé

Potato growing is severely threatened by the late blight agent Phytophthora infestans, which is usually controlled by massive amounts of fungicides. While variety resistance is often bypassed by the pathogen, the plant innate immunity opens the way to new biological plant protection tools, e.g. the COS-OGA elicitor. This oligosaccharide composition mimics the interaction between plants and fungal pathogens as it combines chitosan oligomers (COS) with pectin-derived oligogalacturonides (OGA). Two different COS-OGA elicitors were evaluated against potato late blight: FytoSave, mainly efficient against powdery mildews, and FytoSol, a new composition still under development. In addition, a comparative study of plant defence induction was performed, focusing on the effect of repeated sprayings as well as on the stimulation of salicylic acid (SA), jasmonic acid and ethylene-related pathways during the biotrophic and necrotrophic growth stages of the pathogen. The FytoSave elicitor strongly increased the SA content but failed to induce sufficient protection against late blight, while FytoSol maintained or even decreased the free SA content in the presence of P. infestans and was completely efficient. Surprisingly, the necrotrophic development of P. infestans occurred along with a strong leaf accumulation of free SA and SA-related transcripts. This may represent an attempt by P. infestans to divert plant defences for its own benefit. Preventive sprayings with FytoSol but not FytoSave completely impeded this hijacking. FytoSol seemed to keep the SA pathway under control, thereby preventing its diversion by P. infestans.

langue originale	Anglais
Pages (de - à)	1901-1911
Nombre de pages	11
journal	Plant Pathology
Volume	67
Numéro de publication	9
Les DOIs	https://doi.org/10.1111/ppa.12908
Etat de la publication	Publié - 1 déc. 2018

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10.1111/ppa.12908License: CC BY

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@article{1c6db9af4e9a42438ee0358781c12d65,

title = "Oligosaccharides successfully thwart hijacking of the salicylic acid pathway by Phytophthora infestans in potato leaves",

abstract = "Potato growing is severely threatened by the late blight agent Phytophthora infestans, which is usually controlled by massive amounts of fungicides. While variety resistance is often bypassed by the pathogen, the plant innate immunity opens the way to new biological plant protection tools, e.g. the COS-OGA elicitor. This oligosaccharide composition mimics the interaction between plants and fungal pathogens as it combines chitosan oligomers (COS) with pectin-derived oligogalacturonides (OGA). Two different COS-OGA elicitors were evaluated against potato late blight: FytoSave, mainly efficient against powdery mildews, and FytoSol, a new composition still under development. In addition, a comparative study of plant defence induction was performed, focusing on the effect of repeated sprayings as well as on the stimulation of salicylic acid (SA), jasmonic acid and ethylene-related pathways during the biotrophic and necrotrophic growth stages of the pathogen. The FytoSave elicitor strongly increased the SA content but failed to induce sufficient protection against late blight, while FytoSol maintained or even decreased the free SA content in the presence of P. infestans and was completely efficient. Surprisingly, the necrotrophic development of P. infestans occurred along with a strong leaf accumulation of free SA and SA-related transcripts. This may represent an attempt by P. infestans to divert plant defences for its own benefit. Preventive sprayings with FytoSol but not FytoSave completely impeded this hijacking. FytoSol seemed to keep the SA pathway under control, thereby preventing its diversion by P. infestans.",

keywords = "COS-OGA, oligosaccharides, plant innate immunity, potato late blight, salicylic acid, Solanum tuberosum",

author = "{van Aubel}, G. and S. Serderidis and J. Ivens and A. Clinckemaillie and A. Legr{\`e}ve and B. Hause and {Van Cutsem}, P.",

note = "Funding Information: This work was financially supported by the Service Public de Wallonie, Belgium (DGO3 research grant no. D31-1339). The authors thank Jean-Louis Rolot and Vincent C{\'e}sar from CRA-W Libramont, Belgium for kindly providing the P. infestans strain used in the trial. The authors also thank Gerd Balcke (IPB Halle) for help with LC-MS measurements. G.V.A. and P.V.C. are members of FytoFend SA (Belgium). COS-OGA is a patented product which is commercially available or upon request to FytoFend SA (http://www.fytofend.com/). Funding Information: This work was financially supported by the Service Public de Wallonie, Belgium (DGO3 research grant no. D31-1339). The authors thank Jean-Louis Rolot and Vincent C?sar from CRA-W Libramont, Belgium for kindly providing the P.?infestans strain used in the trial. The authors also thank Gerd Balcke (IPB Halle) for help with LC-MS measurements. G.V.A. and P.V.C. are members of FytoFend SA (Belgium). COS-OGA is a patented product which is commercially available or upon request to FytoFend SA (http://www.fytofend.com/). Publisher Copyright: {\textcopyright} 2018 The Authors. Plant Pathology published by John Wiley & Sons Ltd on behalf of British Society for Plant Pathology. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

year = "2018",

month = dec,

day = "1",

doi = "10.1111/ppa.12908",

language = "English",

volume = "67",

pages = "1901--1911",

journal = "Plant Pathology",

issn = "0032-0862",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "9",

}

TY - JOUR

T1 - Oligosaccharides successfully thwart hijacking of the salicylic acid pathway by Phytophthora infestans in potato leaves

AU - van Aubel, G.

AU - Serderidis, S.

AU - Ivens, J.

AU - Clinckemaillie, A.

AU - Legrève, A.

AU - Hause, B.

AU - Van Cutsem, P.

N1 - Funding Information: This work was financially supported by the Service Public de Wallonie, Belgium (DGO3 research grant no. D31-1339). The authors thank Jean-Louis Rolot and Vincent César from CRA-W Libramont, Belgium for kindly providing the P. infestans strain used in the trial. The authors also thank Gerd Balcke (IPB Halle) for help with LC-MS measurements. G.V.A. and P.V.C. are members of FytoFend SA (Belgium). COS-OGA is a patented product which is commercially available or upon request to FytoFend SA (http://www.fytofend.com/). Funding Information: This work was financially supported by the Service Public de Wallonie, Belgium (DGO3 research grant no. D31-1339). The authors thank Jean-Louis Rolot and Vincent C?sar from CRA-W Libramont, Belgium for kindly providing the P.?infestans strain used in the trial. The authors also thank Gerd Balcke (IPB Halle) for help with LC-MS measurements. G.V.A. and P.V.C. are members of FytoFend SA (Belgium). COS-OGA is a patented product which is commercially available or upon request to FytoFend SA (http://www.fytofend.com/). Publisher Copyright: © 2018 The Authors. Plant Pathology published by John Wiley & Sons Ltd on behalf of British Society for Plant Pathology. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Potato growing is severely threatened by the late blight agent Phytophthora infestans, which is usually controlled by massive amounts of fungicides. While variety resistance is often bypassed by the pathogen, the plant innate immunity opens the way to new biological plant protection tools, e.g. the COS-OGA elicitor. This oligosaccharide composition mimics the interaction between plants and fungal pathogens as it combines chitosan oligomers (COS) with pectin-derived oligogalacturonides (OGA). Two different COS-OGA elicitors were evaluated against potato late blight: FytoSave, mainly efficient against powdery mildews, and FytoSol, a new composition still under development. In addition, a comparative study of plant defence induction was performed, focusing on the effect of repeated sprayings as well as on the stimulation of salicylic acid (SA), jasmonic acid and ethylene-related pathways during the biotrophic and necrotrophic growth stages of the pathogen. The FytoSave elicitor strongly increased the SA content but failed to induce sufficient protection against late blight, while FytoSol maintained or even decreased the free SA content in the presence of P. infestans and was completely efficient. Surprisingly, the necrotrophic development of P. infestans occurred along with a strong leaf accumulation of free SA and SA-related transcripts. This may represent an attempt by P. infestans to divert plant defences for its own benefit. Preventive sprayings with FytoSol but not FytoSave completely impeded this hijacking. FytoSol seemed to keep the SA pathway under control, thereby preventing its diversion by P. infestans.

AB - Potato growing is severely threatened by the late blight agent Phytophthora infestans, which is usually controlled by massive amounts of fungicides. While variety resistance is often bypassed by the pathogen, the plant innate immunity opens the way to new biological plant protection tools, e.g. the COS-OGA elicitor. This oligosaccharide composition mimics the interaction between plants and fungal pathogens as it combines chitosan oligomers (COS) with pectin-derived oligogalacturonides (OGA). Two different COS-OGA elicitors were evaluated against potato late blight: FytoSave, mainly efficient against powdery mildews, and FytoSol, a new composition still under development. In addition, a comparative study of plant defence induction was performed, focusing on the effect of repeated sprayings as well as on the stimulation of salicylic acid (SA), jasmonic acid and ethylene-related pathways during the biotrophic and necrotrophic growth stages of the pathogen. The FytoSave elicitor strongly increased the SA content but failed to induce sufficient protection against late blight, while FytoSol maintained or even decreased the free SA content in the presence of P. infestans and was completely efficient. Surprisingly, the necrotrophic development of P. infestans occurred along with a strong leaf accumulation of free SA and SA-related transcripts. This may represent an attempt by P. infestans to divert plant defences for its own benefit. Preventive sprayings with FytoSol but not FytoSave completely impeded this hijacking. FytoSol seemed to keep the SA pathway under control, thereby preventing its diversion by P. infestans.

KW - COS-OGA

KW - oligosaccharides

KW - plant innate immunity

KW - potato late blight

KW - salicylic acid

KW - Solanum tuberosum

UR - http://www.scopus.com/inward/record.url?scp=85051066337&partnerID=8YFLogxK

U2 - 10.1111/ppa.12908

DO - 10.1111/ppa.12908

M3 - Article

AN - SCOPUS:85051066337

SN - 0032-0862

VL - 67

SP - 1901

EP - 1911

JO - Plant Pathology

JF - Plant Pathology

IS - 9

ER -

Oligosaccharides successfully thwart hijacking of the salicylic acid pathway by Phytophthora infestans in potato leaves

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