Résumé
Upon illumination by ultraviolet light, many animal species emit light through fluorescence processes arising from fluorophores embedded within their biological tissues. Fluorescence studies in living organisms are however relatively scarce and so far limited to the linear regime. Multiphoton excitation fluorescence analyses as well as nonlinear optical techniques offer unique possibilities to investigate the effects of the local environment on the excited states of fluorophores. Herein, these techniques are applied for the first time to study of the naturally controlled fluorescence in insects. The case of the male Hoplia coerulea beetle is investigated because the scales covering the beetle's elytra are known to possess an internal photonic structure with embedded fluorophores, which controls both the beetle's coloration and the fluorescence emission. An intense two-photon excitation fluorescence signal is observed, the intensity of which changes upon contact with water. A third-harmonic generation signal is also detected, the intensity of which depends on the light polarization state. The analysis of these nonlinear optical and fluorescent responses unveils the multi-excited states character of the fluorophore molecules embedded in the beetle's elytra. The role of form anisotropy in the photonic structure, which causes additional tailoring of the beetle's optical responses, is demonstrated by circularly polarized light and nonlinear optical measurements.
| langue originale | Anglais |
|---|---|
| Numéro d'article | 20180052 |
| Pages (de - à) | 20180052 |
| Nombre de pages | 8 |
| journal | Interface focus |
| Volume | 9 |
| Numéro de publication | 1 |
| Les DOIs | |
| état | Publié - 14 déc. 2018 |
Empreinte digitale
Citer ceci
}
Nonlinear optical spectroscopy and two-photon excited fluorescence spectroscopy reveal the excited states of fluorophores embedded in a beetle's elytra. / Mouchet, Sébastien R; Verstraete, Charlotte; Mara, Dimitrije; Van Cleuvenbergen, Stijn; Finlayson, Ewan D; Van Deun, Rik; Deparis, Olivier; Verbiest, Thierry; Maes, Bjorn; Vukusic, Pete; Kolaric, Branko.
Dans: Interface focus, Vol 9, Numéro 1, 20180052, 14.12.2018, p. 20180052.Résultats de recherche: Contribution à un journal/une revue › Article
TY - JOUR
T1 - Nonlinear optical spectroscopy and two-photon excited fluorescence spectroscopy reveal the excited states of fluorophores embedded in a beetle's elytra
AU - Mouchet, Sébastien R
AU - Verstraete, Charlotte
AU - Mara, Dimitrije
AU - Van Cleuvenbergen, Stijn
AU - Finlayson, Ewan D
AU - Van Deun, Rik
AU - Deparis, Olivier
AU - Verbiest, Thierry
AU - Maes, Bjorn
AU - Vukusic, Pete
AU - Kolaric, Branko
PY - 2018/12/14
Y1 - 2018/12/14
N2 - Upon illumination by ultraviolet light, many animal species emit light through fluorescence processes arising from fluorophores embedded within their biological tissues. Fluorescence studies in living organisms are however relatively scarce and so far limited to the linear regime. Multiphoton excitation fluorescence analyses as well as nonlinear optical techniques offer unique possibilities to investigate the effects of the local environment on the excited states of fluorophores. Herein, these techniques are applied for the first time to study of the naturally controlled fluorescence in insects. The case of the male Hoplia coerulea beetle is investigated because the scales covering the beetle's elytra are known to possess an internal photonic structure with embedded fluorophores, which controls both the beetle's coloration and the fluorescence emission. An intense two-photon excitation fluorescence signal is observed, the intensity of which changes upon contact with water. A third-harmonic generation signal is also detected, the intensity of which depends on the light polarization state. The analysis of these nonlinear optical and fluorescent responses unveils the multi-excited states character of the fluorophore molecules embedded in the beetle's elytra. The role of form anisotropy in the photonic structure, which causes additional tailoring of the beetle's optical responses, is demonstrated by circularly polarized light and nonlinear optical measurements.
AB - Upon illumination by ultraviolet light, many animal species emit light through fluorescence processes arising from fluorophores embedded within their biological tissues. Fluorescence studies in living organisms are however relatively scarce and so far limited to the linear regime. Multiphoton excitation fluorescence analyses as well as nonlinear optical techniques offer unique possibilities to investigate the effects of the local environment on the excited states of fluorophores. Herein, these techniques are applied for the first time to study of the naturally controlled fluorescence in insects. The case of the male Hoplia coerulea beetle is investigated because the scales covering the beetle's elytra are known to possess an internal photonic structure with embedded fluorophores, which controls both the beetle's coloration and the fluorescence emission. An intense two-photon excitation fluorescence signal is observed, the intensity of which changes upon contact with water. A third-harmonic generation signal is also detected, the intensity of which depends on the light polarization state. The analysis of these nonlinear optical and fluorescent responses unveils the multi-excited states character of the fluorophore molecules embedded in the beetle's elytra. The role of form anisotropy in the photonic structure, which causes additional tailoring of the beetle's optical responses, is demonstrated by circularly polarized light and nonlinear optical measurements.
KW - natural photonics
KW - photonics crystals
KW - fluorescence
KW - two-photon fluorescence
KW - third-harmonic generation
KW - Beetle
U2 - 10.1098/rsfs.2018.0052
DO - 10.1098/rsfs.2018.0052
M3 - Article
C2 - 30603071
VL - 9
SP - 20180052
JO - Interface focus
JF - Interface focus
SN - 2042-8898
IS - 1
M1 - 20180052
ER -