Non-senescent keratinocytes organize in plasma membrane submicrometric lipid domains enriched in sphingomyelin and involved in re-epithelialization

Abdallah Mound, Vesela Lozanova, Céline Warnon, Maryse Hermant, Julie Robic, Christelle Guere, Katell Vie, Catherine Lambert de Rouvroit, Donatienne Tyteca, Florence Debacq-Chainiaux, Yves Poumay

Résultats de recherche: Recherche - Revue par des pairsArticle

Résumé

Membrane lipid raft model has long been debated, but recently the concept of lipid submicrometric domains has emerged to characterize larger (micrometric) and more stable lipid membrane domains. Such domains organize signaling platforms involved in normal or pathological conditions. In this study, adhering human keratinocytes were investigated for their ability to organize such specialized lipid domains. Successful fluorescent probing of lipid domains, by either inserting exogenous sphingomyelin (BODIPY-SM) or using detoxified fragments of lysenin and theta toxins fused to mCherry, allowed specific, sensitive and quantitative detection of sphingomyelin and cholesterol and demonstrated for the first time submicrometric organization of lipid domains in living keratinocytes. Potential functionality of such domains was additionally assessed during replicative senescence, notably through gradual disappearance of SM-rich domains in senescent keratinocytes. Indeed, SM-rich domains were found critical to preserve keratinocyte migration before senescence, because sphingomyelin or cholesterol depletion in keratinocytes significantly alter lipid domains and reduce migration ability.

langueAnglais
Pages958-971
Nombre de pages14
journalBiochimica Biophysica Acta.
Volume1862
Numéro9
Les DOIs
étatPublié - 23 juin 2017

Empreinte digitale

Re-Epithelialization
Sphingomyelins
Membrane Lipids
Keratinocytes
Cell Membrane
Lipids
Cholesterol
Cell Aging
lysenin
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene

mots-clés

    Citer ceci

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    title = "Non-senescent keratinocytes organize in plasma membrane submicrometric lipid domains enriched in sphingomyelin and involved in re-epithelialization",
    abstract = "Membrane lipid raft model has long been debated, but recently the concept of lipid submicrometric domains has emerged to characterize larger (micrometric) and more stable lipid membrane domains. Such domains organize signaling platforms involved in normal or pathological conditions. In this study, adhering human keratinocytes were investigated for their ability to organize such specialized lipid domains. Successful fluorescent probing of lipid domains, by either inserting exogenous sphingomyelin (BODIPY-SM) or using detoxified fragments of lysenin and theta toxins fused to mCherry, allowed specific, sensitive and quantitative detection of sphingomyelin and cholesterol and demonstrated for the first time submicrometric organization of lipid domains in living keratinocytes. Potential functionality of such domains was additionally assessed during replicative senescence, notably through gradual disappearance of SM-rich domains in senescent keratinocytes. Indeed, SM-rich domains were found critical to preserve keratinocyte migration before senescence, because sphingomyelin or cholesterol depletion in keratinocytes significantly alter lipid domains and reduce migration ability.",
    keywords = "Journal Article",
    author = "Abdallah Mound and Vesela Lozanova and Céline Warnon and Maryse Hermant and Julie Robic and Christelle Guere and Katell Vie and {Lambert de Rouvroit}, Catherine and Donatienne Tyteca and Florence Debacq-Chainiaux and Yves Poumay",
    note = "Copyright © 2017. Published by Elsevier B.V.",
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    Non-senescent keratinocytes organize in plasma membrane submicrometric lipid domains enriched in sphingomyelin and involved in re-epithelialization. / Mound, Abdallah; Lozanova, Vesela; Warnon, Céline; Hermant, Maryse; Robic, Julie; Guere, Christelle; Vie, Katell; Lambert de Rouvroit, Catherine; Tyteca, Donatienne; Debacq-Chainiaux, Florence; Poumay, Yves.

    Dans: Biochimica Biophysica Acta. , Vol 1862, Numéro 9, 23.06.2017, p. 958-971.

    Résultats de recherche: Recherche - Revue par des pairsArticle

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    AU - Mound,Abdallah

    AU - Lozanova,Vesela

    AU - Warnon,Céline

    AU - Hermant,Maryse

    AU - Robic,Julie

    AU - Guere,Christelle

    AU - Vie,Katell

    AU - Lambert de Rouvroit,Catherine

    AU - Tyteca,Donatienne

    AU - Debacq-Chainiaux,Florence

    AU - Poumay,Yves

    N1 - Copyright © 2017. Published by Elsevier B.V.

    PY - 2017/6/23

    Y1 - 2017/6/23

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