TY - JOUR
T1 - Neuropeptidome of the Cephalopod Sepia officinalis
T2 - Identification, Tissue Mapping, and Expression Pattern of Neuropeptides and Neurohormones during Egg Laying
AU - Zatylny-Gaudin, Céline
AU - Cornet, Valérie
AU - Leduc, Alexandre
AU - Zanuttini, Bruno
AU - Corre, Erwan
AU - Le Corguillé, Gildas
AU - Bernay, Benoît
AU - Garderes, Johan
AU - Kraut, Alexandra
AU - Couté, Yohan
AU - Henry, Joël
PY - 2016
Y1 - 2016
N2 - Cephalopods exhibit a wide variety of behaviors such as prey capture, communication, camouflage, and reproduction thanks to a complex central nervous system (CNS) divided into several functional lobes that express a wide range of neuropeptides involved in the modulation of behaviors and physiological mechanisms associated with the main stages of their life cycle. This work focuses on the neuropeptidome expressed during egg-laying through de novo construction of the CNS transcriptome using an RNAseq approach (Illumina sequencing). Then, we completed the in silico analysis of the transcriptome by characterizing and tissue-mapping neuropeptides by mass spectrometry. To identify neuropeptides involved in the egg-laying process, we determined (1) the neuropeptide contents of the neurohemal area, hemolymph (blood), and nerve endings in mature females and (2) the expression levels of these peptides. Among the 38 neuropeptide families identified from 55 transcripts, 30 were described for the first time in Sepia officinalis, 5 were described for the first time in the animal kingdom, and 14 were strongly overexpressed in egg-laying females as compared with mature males. Mass spectrometry screening of hemolymph and nerve ending contents allowed us to clarify the status of many neuropeptides, that is, to determine whether they were neuromodulators or neurohormones.
AB - Cephalopods exhibit a wide variety of behaviors such as prey capture, communication, camouflage, and reproduction thanks to a complex central nervous system (CNS) divided into several functional lobes that express a wide range of neuropeptides involved in the modulation of behaviors and physiological mechanisms associated with the main stages of their life cycle. This work focuses on the neuropeptidome expressed during egg-laying through de novo construction of the CNS transcriptome using an RNAseq approach (Illumina sequencing). Then, we completed the in silico analysis of the transcriptome by characterizing and tissue-mapping neuropeptides by mass spectrometry. To identify neuropeptides involved in the egg-laying process, we determined (1) the neuropeptide contents of the neurohemal area, hemolymph (blood), and nerve endings in mature females and (2) the expression levels of these peptides. Among the 38 neuropeptide families identified from 55 transcripts, 30 were described for the first time in Sepia officinalis, 5 were described for the first time in the animal kingdom, and 14 were strongly overexpressed in egg-laying females as compared with mature males. Mass spectrometry screening of hemolymph and nerve ending contents allowed us to clarify the status of many neuropeptides, that is, to determine whether they were neuromodulators or neurohormones.
KW - Amino Acid Sequence
KW - Animals
KW - Female
KW - Molecular Sequence Annotation
KW - Molecular Sequence Data
KW - Neuropeptides
KW - Neurotransmitter Agents
KW - Organ Specificity
KW - Oviposition
KW - Proteome
KW - RNA, Messenger
KW - Sepia
KW - Transcriptome
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1021/acs.jproteome.5b00463
DO - 10.1021/acs.jproteome.5b00463
M3 - Article
C2 - 26632866
SN - 1535-3893
VL - 15
SP - 48
EP - 67
JO - Journal of Proteome Research
JF - Journal of Proteome Research
IS - 1
ER -