Myeloperoxidase-Oxidized LDLs Enhance an Anti-Inflammatory M2 and Antioxidant Phenotype in Murine Macrophages

Valérie Pireaux, Aude Sauvage, Benoît Bihin, Martine Van Steenbrugge, Alexandre Rousseau, Pierre Van Antwerpen, Karim Zouaoui Boudjeltia, Martine Raes

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

Macrophages and oxidized LDLs play a key role in atherogenesis but their heterogeneity has been neglected up to now. Macrophages are prone to polarization and subsets of polarized macrophages have been described in atheromas. LDLs can be oxidized not only chemically by copper (Ox-LDLs) but also enzymatically by myeloperoxidase (MpOx-LDLs) resulting in oxidized LDLs poor in lipid peroxides. The effects of physiologically relevant myeloperoxidase-oxidized LDLs on macrophage polarization or on polarized macrophages remain largely unknown. In this study, the effects of LDLs on macrophage polarization were investigated by monitoring the expression of M1 and M2 genes following stimulation with native LDLs, Ox-LDLs, or MpOx-LDLs in RAW 264.7 cells. Except for MRC1, which is induced only by Ox-LDLs, MpOx-LDLs induced an overexpression of most of the selected marker genes at the mRNA level. MpOx-LDLs also modulate marker gene expression in polarized macrophages favoring notably anti-inflammatory Arg1 expression in M2 cells and also in the other phenotypes. Noteworthy, MpOx-LDLs were the most efficient to accumulate lipids intracellularly in (un)polarized macrophages whatever the phenotype. These data were largely confirmed in murine bone marrow-derived macrophages. Our data suggest that MpOx-LDLs were the most efficient to accumulate within cells and to enhance an anti-inflammatory and antioxidant phenotype in M2 cells and also in the other macrophage phenotypes.

langue originaleAnglais
Numéro d'article8249476
journalMediators of Inflammation
Volume2016
Les DOIs
Etat de la publicationPublié - 2016

Empreinte digitale Examiner les sujets de recherche de « Myeloperoxidase-Oxidized LDLs Enhance an Anti-Inflammatory M2 and Antioxidant Phenotype in Murine Macrophages ». Ensemble, ils forment une empreinte digitale unique.

  • Équipement

    BD Pathway 855

    Charles Nicaise (!!Manager) & Francesca Cecchet (!!Manager)

    Plateforme technologique Morphologie, imagerie

    Equipement/installations: Equipement

  • FACS

    Charles Nicaise (!!Manager) & Francesca Cecchet (!!Manager)

    Plateforme technologique Morphologie, imagerie

    Equipement/installations: Equipement

  • Génétique - génomique

    Karine Van Doninck (!!Manager)

    Plateforme technologique Genetique et genomique

    Equipement/installations: Plateforme technolgique

  • Thèses de l'étudiant

    Athérosclérose: importance de la polarisation des macrophages et des LDLs oxydées

    Author: Pireaux, V., 17 mai 2017

    Superviseur: Raes, M. (Promoteur), Zouaoui Boudjeltia, K. (Personne externe) (Copromoteur), Poumay, Y. (Président), Michiels, C. (Jury), Lesnik, P. (Personne externe) (Jury) & Moser, M. (Personne externe) (Jury)

    Thèse de l'étudiant: Doc typesDocteur en Sciences

    Fichier

    Contient cette citation