An electrochemical sensor for the amperometric determination of l-tryptophan (Trp) was fabricated by modifying the carbon paste electrode (CPE) with multi-walled carbon nanotubes (MWCNTs) using drop cast method. 4.0μL of the dispersion containing 2.0mg of MWCNTs in 1.0mL of ethanol was drop cast onto the electrode surface and dried in hot air oven to form a stable layer of MWCNTs. The electro-catalytic activity of the modified electrode towards the oxidation of Trp was thoroughly investigated. The modification with MWCNTs has greatly improved the current sensitivity of CPE for the oxidation of Trp. A very minimal amount of the modifier was required to achieve such a high sensitivity. The field emission scanning electron microscopy (FESEM) images revealed a uniform coverage of the surface of CPE by MWCNTs. Nyquist plots revealed the least charge transfer resistance for the modified electrode. The analytical performance of the modified electrode was examined using amperometry under hydro-dynamic conditions. The two linear dynamic ranges observed for Trp were 0.6-9.0μM and 10.0-100.0μM. The amperometric determination of Trp did not suffer any interference from other biomolecules. The detection limit of Trp at modified electrode was (3.30±0.37)×10-8M (S/N=3). The analytical applications of the modified electrode were demonstrated by estimating Trp in the spiked milk and biological fluid such as blood serum. The modified electrode showed good reproducibility, long-term stability and anti-fouling effects.