Multi-layered plasma polymerized chips for SPR-based detection

Ram Prasad Gandhiraman, Nam Cao Hoai Le, Chandra K. Dixit, Cedric Volcke, Colin Doyle, Vladimir Gubala, Ruairi Monaghan, Bryony James, Richard O'Kennedy, Stephen Daniels, David E. Williams

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

The surface functionalization of a noble metal is crucial in a surface plasmon resonance-based biomolecular detection system because the interfacial coating must retain the activity of immobilized biomolecules while enhancing the optimal loading. We present here a one-step, room-temperature, high-speed, gas-phase plasma polymerization process for functionalizing gold substrates using siloxane as an adhesion layer and acrylic acid as a functional layer. Siloxane- and thiol-based coatings were compared for their performance as adhesion and the interfacial layer for subsequent functionalization. An in situ sequential deposition of siloxane and acrylic acid resulted in a 7-fold increase in carboxylic functionality surfacial content compared to films deposited with thiol-containing precursors. Grading of the layer composition achieved as a consequence of ion-induced mixing on the surface coating under the application of the plasma is confirmed through secondary ion mass spectroscopic studies. DNA hybridization assays were demonstrated on gold/glass substrates using surface plasmon enhanced ellipsometry and the applicability of this coating for protein immunoassays were demonstrated with plasma functionalized gold/plastic substrates in Biacore 3000 SPR instrument.
langue originaleAnglais
Pages (de - à)4640–4648
journalACS Applied Materials and Interfaces
Volume3
Numéro de publication12
Les DOIs
étatPublié - 2011

Empreinte digitale

Siloxanes
Gold
Plasmas
Coatings
Sulfhydryl Compounds
Acrylics
Substrates
Adhesion
Ions
Plasma polymerization
Acids
Ellipsometry
Surface plasmon resonance
Biomolecules
Precious metals
Assays
DNA
Gases
Plastics
Proteins

Citer ceci

Gandhiraman, R. P., Le, N. C. H., Dixit, C. K., Volcke, C., Doyle, C., Gubala, V., ... Williams, D. E. (2011). Multi-layered plasma polymerized chips for SPR-based detection. ACS Applied Materials and Interfaces, 3(12), 4640–4648. https://doi.org/10.1021/am201061k
Gandhiraman, Ram Prasad ; Le, Nam Cao Hoai ; Dixit, Chandra K. ; Volcke, Cedric ; Doyle, Colin ; Gubala, Vladimir ; Monaghan, Ruairi ; James, Bryony ; O'Kennedy, Richard ; Daniels, Stephen ; Williams, David E. / Multi-layered plasma polymerized chips for SPR-based detection. Dans: ACS Applied Materials and Interfaces. 2011 ; Vol 3, Numéro 12. p. 4640–4648.
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abstract = "The surface functionalization of a noble metal is crucial in a surface plasmon resonance-based biomolecular detection system because the interfacial coating must retain the activity of immobilized biomolecules while enhancing the optimal loading. We present here a one-step, room-temperature, high-speed, gas-phase plasma polymerization process for functionalizing gold substrates using siloxane as an adhesion layer and acrylic acid as a functional layer. Siloxane- and thiol-based coatings were compared for their performance as adhesion and the interfacial layer for subsequent functionalization. An in situ sequential deposition of siloxane and acrylic acid resulted in a 7-fold increase in carboxylic functionality surfacial content compared to films deposited with thiol-containing precursors. Grading of the layer composition achieved as a consequence of ion-induced mixing on the surface coating under the application of the plasma is confirmed through secondary ion mass spectroscopic studies. DNA hybridization assays were demonstrated on gold/glass substrates using surface plasmon enhanced ellipsometry and the applicability of this coating for protein immunoassays were demonstrated with plasma functionalized gold/plastic substrates in Biacore 3000 SPR instrument.",
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Gandhiraman, RP, Le, NCH, Dixit, CK, Volcke, C, Doyle, C, Gubala, V, Monaghan, R, James, B, O'Kennedy, R, Daniels, S & Williams, DE 2011, 'Multi-layered plasma polymerized chips for SPR-based detection', ACS Applied Materials and Interfaces, VOL. 3, Numéro 12, p. 4640–4648. https://doi.org/10.1021/am201061k

Multi-layered plasma polymerized chips for SPR-based detection. / Gandhiraman, Ram Prasad; Le, Nam Cao Hoai; Dixit, Chandra K.; Volcke, Cedric; Doyle, Colin; Gubala, Vladimir; Monaghan, Ruairi; James, Bryony; O'Kennedy, Richard; Daniels, Stephen; Williams, David E.

Dans: ACS Applied Materials and Interfaces, Vol 3, Numéro 12, 2011, p. 4640–4648.

Résultats de recherche: Contribution à un journal/une revueArticle

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T1 - Multi-layered plasma polymerized chips for SPR-based detection

AU - Gandhiraman, Ram Prasad

AU - Le, Nam Cao Hoai

AU - Dixit, Chandra K.

AU - Volcke, Cedric

AU - Doyle, Colin

AU - Gubala, Vladimir

AU - Monaghan, Ruairi

AU - James, Bryony

AU - O'Kennedy, Richard

AU - Daniels, Stephen

AU - Williams, David E.

PY - 2011

Y1 - 2011

N2 - The surface functionalization of a noble metal is crucial in a surface plasmon resonance-based biomolecular detection system because the interfacial coating must retain the activity of immobilized biomolecules while enhancing the optimal loading. We present here a one-step, room-temperature, high-speed, gas-phase plasma polymerization process for functionalizing gold substrates using siloxane as an adhesion layer and acrylic acid as a functional layer. Siloxane- and thiol-based coatings were compared for their performance as adhesion and the interfacial layer for subsequent functionalization. An in situ sequential deposition of siloxane and acrylic acid resulted in a 7-fold increase in carboxylic functionality surfacial content compared to films deposited with thiol-containing precursors. Grading of the layer composition achieved as a consequence of ion-induced mixing on the surface coating under the application of the plasma is confirmed through secondary ion mass spectroscopic studies. DNA hybridization assays were demonstrated on gold/glass substrates using surface plasmon enhanced ellipsometry and the applicability of this coating for protein immunoassays were demonstrated with plasma functionalized gold/plastic substrates in Biacore 3000 SPR instrument.

AB - The surface functionalization of a noble metal is crucial in a surface plasmon resonance-based biomolecular detection system because the interfacial coating must retain the activity of immobilized biomolecules while enhancing the optimal loading. We present here a one-step, room-temperature, high-speed, gas-phase plasma polymerization process for functionalizing gold substrates using siloxane as an adhesion layer and acrylic acid as a functional layer. Siloxane- and thiol-based coatings were compared for their performance as adhesion and the interfacial layer for subsequent functionalization. An in situ sequential deposition of siloxane and acrylic acid resulted in a 7-fold increase in carboxylic functionality surfacial content compared to films deposited with thiol-containing precursors. Grading of the layer composition achieved as a consequence of ion-induced mixing on the surface coating under the application of the plasma is confirmed through secondary ion mass spectroscopic studies. DNA hybridization assays were demonstrated on gold/glass substrates using surface plasmon enhanced ellipsometry and the applicability of this coating for protein immunoassays were demonstrated with plasma functionalized gold/plastic substrates in Biacore 3000 SPR instrument.

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JO - ACS Applied Materials & Interfaces

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