Molecular recognition of the plant hormone auxin is still a puzzle

Biserka Kojic-Prodic, Volker Magnus, Sanja Tomic, Snjezana Antolic, Branka Salopek-Sondi, Eduard Dolusic, Branimir Bertosa

Résultats de recherche: Contribution dans un livre/un catalogue/un rapport/dans les actes d'une conférenceArticle dans les actes d'une conférence/un colloque

Résumé

The plant hormone auxin is involved in almost every aspect of plant development; auxin homeostasis and auxin signaling are thus of crucial importance. The wider auxin family includes endogenous growth promoters, such as indole-3-acetic acid (IAA), and chemically diverse synthetic analogues; minor structural modifications afford growth inhibitors (antiauxins). As proper classification is of technological relevance (growth regulators, herbicides) we developed a classification by interaction similarity indices, based on the evaluation of two distinctive molecular conformations (planar and tilted) of indole-3-acetic acid in comparison with the optimized conformations of 50 compounds. Then we extended the method to a larger set of compounds using similarity of molecular interaction fields and lipophilicity predictions (log P). In addition to theoretical approaches based on the evaluation of molecular conformations of possible relevance for binding to the auxin receptor(s), we used experimental methods, such as X-ray structure analysis and spectroscopic methods to characterize the molecules of interest: alkylated IAAs, halogenated IAAs (F, Cl, Br), and dihalogenated IAAs. Systematic analysis of physico-chemical and structural properties provided indirect insight into the topology of the substrate-binding site of the, so far hypothetical, common auxin receptor. Our findings are in reasonable accord with the models proposed by previous researchers. The question of molecular fit to the receptor active site will, however, remain open until the coordinates of the first structurally characterized auxin binding protein, ABP1, will be in the public domain.
langue originaleAnglais
titreAbstracts, XIX Congress and General Assembly of the International Union of Crystallography, Geneva, Switzerland, 06.-15.08.2002
PagesC139-C139
Nombre de pages1
VolumeA58
Etat de la publicationPublié - 2002
EvénementXIX Congress and General Assembly of the International Union of Crystallography, Geneva, Switzerland, 06.-15.08.2002 - Geneva, Suisse
Durée: 6 août 200215 août 2002

Série de publications

NomActa Crystallographica
VolumeA58

Une conférence

Une conférenceXIX Congress and General Assembly of the International Union of Crystallography, Geneva, Switzerland, 06.-15.08.2002
PaysSuisse
La villeGeneva
période6/08/0215/08/02

Empreinte digitale

Examiner les sujets de recherche de « Molecular recognition of the plant hormone auxin is still a puzzle ». Ensemble, ils forment une empreinte digitale unique.

Contient cette citation