Mitochondria permeability transition-dependent tert-butyl hydroperoxide-induced apoptosis in hepatoma HepG2 cells

J.-P. Piret, T. Arnould, B. Fuks, P. Chatelain, J. Remacle, C. Michiels

Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs

Résumé

Tert-butyl hydroperoxide (t-BHP) has been demonstrated to induce apoptosis in hepatoma cell line HepG2, but poor data were available on the signaling pathway initiated by t-BHP. In this work, we studied in details the apoptotic pathways induced in HepG2 cells by t-BHP. DNA fragmentation, activation of caspases and cytochrome c release were demonstrated. Permeability transition pore inhibitors prevented the DNA fragmentation and caspase activation induced by t-BHP. In addition, changes in the mitochondrial membrane potential were detected: hyperpolarization preceded loss of membrane potential. It also preceded caspase activation which occurred before the induction of DNA fragmentation. Taken together, these results emphasize the central role played by mitochondria in the initiation of apoptosis in HepG2 cells exposed to oxidant agents.
langue originaleAnglais
Pages (de - à)611-620
Nombre de pages10
journalBiochemical Pharmacology
Volume67
Numéro de publication4
Les DOIs
Etat de la publicationPublié - 2004

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